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Article: The effect of the EAAEAE insert on the property of human metallothionein-3

TitleThe effect of the EAAEAE insert on the property of human metallothionein-3
Authors
Zheng, QYang, WMYu, WHCai, BTeng, XCXie, YSun, HZZhang, MJHuang, ZX
KeywordsEAAEAE insert
Human metallothionein-3
Neuron growth inhibitory factor
Issue Date2003
PublisherOxford University Press. The Journal's web site is located at http://peds.oxfordjournals.org/
Citation
Protein Engineering, 2003, v. 16 n. 12, p. 865-870 How to Cite?
DOI: http://dx.doi.org/10.1093/protein/gzg127
AbstractMT3 shows apparently different properties and function from MT1 even though they have 70% sequence homology. Possibly the two inserts, Thr5 and a negatively charged hexapeptide at position-55 in MT3, play important roles. A series of MT3 variants around the EAAEAE hexapeptide have been prepared by site-directed mutagenesis and their properties and reactivity towards pH, EDTA and DTNB have been studied. Our detailed studies revealed that the EAAEAE insert is essential to the property of MT3. It is the hexapeptide insert, to some extent, making the MT3 α-domain looser and lower stability of the metal-thiolate cluster, which could be accessed more easily.
Persistent Identifierhttp://hdl.handle.net/10722/68805
ISSN
0269-2139
ISI Accession Number ID
WOS:000220520400002
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorZheng, Qen_HK
dc.contributor.authorYang, WMen_HK
dc.contributor.authorYu, WHen_HK
dc.contributor.authorCai, Ben_HK
dc.contributor.authorTeng, XCen_HK
dc.contributor.authorXie, Yen_HK
dc.contributor.authorSun, HZen_HK
dc.contributor.authorZhang, MJen_HK
dc.contributor.authorHuang, ZXen_HK
dc.date.accessioned2010-09-06T06:07:53Z-
dc.date.available2010-09-06T06:07:53Z-
dc.date.issued2003en_HK
dc.identifier.citationProtein Engineering, 2003, v. 16 n. 12, p. 865-870en_HK
dc.identifier.issn0269-2139en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68805-
dc.description.abstractMT3 shows apparently different properties and function from MT1 even though they have 70% sequence homology. Possibly the two inserts, Thr5 and a negatively charged hexapeptide at position-55 in MT3, play important roles. A series of MT3 variants around the EAAEAE hexapeptide have been prepared by site-directed mutagenesis and their properties and reactivity towards pH, EDTA and DTNB have been studied. Our detailed studies revealed that the EAAEAE insert is essential to the property of MT3. It is the hexapeptide insert, to some extent, making the MT3 α-domain looser and lower stability of the metal-thiolate cluster, which could be accessed more easily.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://peds.oxfordjournals.org/en_HK
dc.relation.ispartofProtein Engineeringen_HK
dc.rightsProtein Engineering. Copyright © Oxford University Press.en_HK
dc.subjectEAAEAE inserten_HK
dc.subjectHuman metallothionein-3en_HK
dc.subjectNeuron growth inhibitory factoren_HK
dc.titleThe effect of the EAAEAE insert on the property of human metallothionein-3en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0269-2139&volume=16&spage=865&epage=870&date=2003&atitle=The+effect+of+the+EAAEAE+insert+on+the+property+of+human+metallothionein-3+en_HK
dc.identifier.emailSun, HZ:hsun@hkucc.hku.hken_HK
dc.identifier.authoritySun, HZ=rp00777en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/protein/gzg127-
dc.identifier.pmid14983064-
dc.identifier.scopuseid_2-s2.0-1442310628en_HK
dc.identifier.hkuros94261en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1442310628&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue12en_HK
dc.identifier.spage865en_HK
dc.identifier.epage870en_HK
dc.identifier.isiWOS:000220520400002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZheng, Q=36887872800en_HK
dc.identifier.scopusauthoridYang, WM=7407760358en_HK
dc.identifier.scopusauthoridYu, WH=55205334100en_HK
dc.identifier.scopusauthoridCai, B=36484162900en_HK
dc.identifier.scopusauthoridTeng, XC=9842935500en_HK
dc.identifier.scopusauthoridXie, Y=36121797300en_HK
dc.identifier.scopusauthoridSun, HZ=7404827446en_HK
dc.identifier.scopusauthoridZhang, MJ=7601555100en_HK
dc.identifier.scopusauthoridHuang, ZX=7406221847en_HK
dc.identifier.issnl0269-2139-

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