File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.2166/ws.2006.968
- Scopus: eid_2-s2.0-33847076515
- Find via
Supplementary
-
Citations:
- Scopus: 0
- Appears in Collections:
Article: Protein profiles of extracellular polymeric substances and activated sludge in a membrane biological reactor by 2-dimensional gel electrophoresis
Title | Protein profiles of extracellular polymeric substances and activated sludge in a membrane biological reactor by 2-dimensional gel electrophoresis |
---|---|
Authors | |
Keywords | Activated sludge Extracellular polymeric substances (EPS) Membrane biological reactor (MBR) Two-dimensional gel electrophoresis (2-DGE) |
Issue Date | 2006 |
Publisher | I W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst_ws |
Citation | Water Science And Technology: Water Supply, 2006, v. 6 n. 6, p. 27-33 How to Cite? |
Abstract | Extracellular polymeric substances (EPS) play a key role in wastewater treatment, specifically flocculation, settling and treatment efficiency. Proteins are the predominant components in EPS and influence hydrophobicity and flocculation of sludge. In this paper, two-dimensional gel electrophoresis (2-DGE) technique was used to analyse protein profiles of EPS from a membrane biological reactor (MBR) fed either glucose or acetate. A highly reproducible 2-DGE protocol was developed achieving >95% of protein spots matches in repeated runs. Results also showed that proteins of EPS obtained from the activated sludge displayed a similar protein pattern between pH 4-7 with molecular weight 10-80 kD. Protein profiles of EPS extracted from activated sludge fed glucose and acetate showed similar distribution patterns for proteins, but further separation of proteins between pH 4-7 revealed distinctive differences between the specific proteins from these two carbon sources. In addition, information of 2-DGE protein profiles indicates little relationship between the physical and chemical analyses. It is apparent that 2-DGE is a much more powerful and a very useful tool in studying metabolisms of microbial communities at the level of metaproteomics. © IWA Publishing 2006. |
Persistent Identifier | http://hdl.handle.net/10722/71011 |
ISSN | 2023 Impact Factor: 1.9 2023 SCImago Journal Rankings: 0.452 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xie, B | en_HK |
dc.contributor.author | Gu, JD | en_HK |
dc.contributor.author | Li, XY | en_HK |
dc.date.accessioned | 2010-09-06T06:28:07Z | - |
dc.date.available | 2010-09-06T06:28:07Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | Water Science And Technology: Water Supply, 2006, v. 6 n. 6, p. 27-33 | en_HK |
dc.identifier.issn | 1606-9749 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/71011 | - |
dc.description.abstract | Extracellular polymeric substances (EPS) play a key role in wastewater treatment, specifically flocculation, settling and treatment efficiency. Proteins are the predominant components in EPS and influence hydrophobicity and flocculation of sludge. In this paper, two-dimensional gel electrophoresis (2-DGE) technique was used to analyse protein profiles of EPS from a membrane biological reactor (MBR) fed either glucose or acetate. A highly reproducible 2-DGE protocol was developed achieving >95% of protein spots matches in repeated runs. Results also showed that proteins of EPS obtained from the activated sludge displayed a similar protein pattern between pH 4-7 with molecular weight 10-80 kD. Protein profiles of EPS extracted from activated sludge fed glucose and acetate showed similar distribution patterns for proteins, but further separation of proteins between pH 4-7 revealed distinctive differences between the specific proteins from these two carbon sources. In addition, information of 2-DGE protein profiles indicates little relationship between the physical and chemical analyses. It is apparent that 2-DGE is a much more powerful and a very useful tool in studying metabolisms of microbial communities at the level of metaproteomics. © IWA Publishing 2006. | en_HK |
dc.language | eng | en_HK |
dc.publisher | I W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst_ws | en_HK |
dc.relation.ispartof | Water Science and Technology: Water Supply | en_HK |
dc.subject | Activated sludge | en_HK |
dc.subject | Extracellular polymeric substances (EPS) | en_HK |
dc.subject | Membrane biological reactor (MBR) | en_HK |
dc.subject | Two-dimensional gel electrophoresis (2-DGE) | en_HK |
dc.title | Protein profiles of extracellular polymeric substances and activated sludge in a membrane biological reactor by 2-dimensional gel electrophoresis | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1606-9749&volume=6&issue=6&spage=27&epage=33&date=2007&atitle=Protein+profiles+of+extracellular+polymeric+substances+and+activated+sludge+in+a+membrane+biological+reactor+by+2-dimensional+gel+electrophoresis | en_HK |
dc.identifier.email | Gu, JD: jdgu@hkucc.hku.hk | en_HK |
dc.identifier.email | Li, XY: xlia@hkucc.hku.hk | en_HK |
dc.identifier.authority | Gu, JD=rp00701 | en_HK |
dc.identifier.authority | Li, XY=rp00222 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.2166/ws.2006.968 | en_HK |
dc.identifier.scopus | eid_2-s2.0-33847076515 | en_HK |
dc.identifier.hkuros | 132045 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33847076515&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 6 | en_HK |
dc.identifier.issue | 6 | en_HK |
dc.identifier.spage | 27 | en_HK |
dc.identifier.epage | 33 | en_HK |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Xie, B=15849776300 | en_HK |
dc.identifier.scopusauthorid | Gu, JD=7403129601 | en_HK |
dc.identifier.scopusauthorid | Li, XY=26642887900 | en_HK |
dc.identifier.issnl | 1606-9749 | - |