Cardiac responses to abiotic factors in two tropical limpets, occurring at different levels of the shore

Abstract

Laboratory experiments tested whether the cardiac activity of two species of limpets (Cellana spp.), co-occurring on the rocky shores of Hong Kong but having different zonal distributions, was differently affected by variations in physical environmental characteristics. Experiments reproduced exposure of limpets to dehydration, high water temperature and reduced salinity, all conditions that can occasionally be faced by animals during low tides. Cardiographic traces were obtained using a non-invasive method based on infrared light sensors glued onto the limpet shell in a position directly over the heart. The signals were filtered and amplified, viewed on portable oscilloscopes and automatically transferred to computer. When tested under acclimation temperatures, the lower shore C. toreuma had faster heart rates than the upper shore C. grata, both in air and when submerged in seawater. Regular alternation of heartbeating and acardia were observed in C. grata exposed to both dry and saturated air, whilst C. toreuma showed transient acardia only during exposure to dry air. Both species showed a similar increase in heart rate with increasing temperature (Q10∼1.8 in the range 25-34°C). When submerged in freshwater, C. grata regularly alternated acardia with beating at a rate slightly lower than in seawater, whilst C. toreuma showed strong bradycardia with reduction of beat amplitude, followed by steady acardia. Recovery of heart-beating after return to initial salinity was faster in C. grata, whilst C. toreuma, initially bradycardic, showed a consistent return to tachycardia. Overall, this study shows that the two congeneric species exhibit different cardiac responses to variations in environmental characteristics, which may be related to their differing vertical distribution.