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- Publisher Website: 10.1016/j.ydbio.2004.09.032
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- PMID: 15572152
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Article: Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos
Title | Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos |
---|---|
Authors | |
Keywords | BMP Chordin Differentiation Gene expression Hematopoiesis Microarray Morpholino Zebrafish |
Issue Date | 2005 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio |
Citation | Developmental Biology, 2005, v. 277 n. 1, p. 235-254 How to Cite? |
Abstract | We investigated the mechanisms of intermediate cell mass (ICM) expansion in zebrafish chordin (Chd) morphant embryos and examined the role of BMPs in relation to this phenotype. At 24 h post-fertilization (hpf), the expanded ICM of embryos injected with chd morpholino (MO) (Chd MO embryos) contained a monotonous population of hematopoietic progenitors. In situ hybridization showed that hematopoietic transcription factors were ubiquitously expressed in the ICM whereas vascular gene expression was confined to the periphery. BMP4 (but not BMP2b or 7) and smad5 mRNA were ectopically expressed in the Chd MO ICM. At 48 hpf, monocytic cells were evident in both the ICM and circulation of Chd MO but not WT embryos. While injection of BMP4 MO had no effect on WT hematopoiesis, co-injecting BMP4 with chd MOs significantly reduced ICM expansion. Microarray studies revealed a number of genes that were differentially expressed in Chd MO and WT embryos and their roles in hematopoiesis has yet to be determined. In conclusion, the expanded ICM in Chd MO embryos represented an expansion of embryonic hematopoiesis that was skewed towards a monocytic lineage. BMP4, but not BMP2b or 7, was involved in this process. The results provide ground for further research into the mechanisms of embryonic hematopoietic cell expansion. © 2004 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/76317 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 1.147 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Leung, AYH | en_HK |
dc.contributor.author | Mendenhall, EM | en_HK |
dc.contributor.author | Kwan, TTF | en_HK |
dc.contributor.author | Liang, R | en_HK |
dc.contributor.author | Eckfeldt, C | en_HK |
dc.contributor.author | Chen, E | en_HK |
dc.contributor.author | Hammerschmidt, M | en_HK |
dc.contributor.author | Grindley, S | en_HK |
dc.contributor.author | Ekker, SC | en_HK |
dc.contributor.author | Verfaillie, CM | en_HK |
dc.date.accessioned | 2010-09-06T07:19:57Z | - |
dc.date.available | 2010-09-06T07:19:57Z | - |
dc.date.issued | 2005 | en_HK |
dc.identifier.citation | Developmental Biology, 2005, v. 277 n. 1, p. 235-254 | en_HK |
dc.identifier.issn | 0012-1606 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/76317 | - |
dc.description.abstract | We investigated the mechanisms of intermediate cell mass (ICM) expansion in zebrafish chordin (Chd) morphant embryos and examined the role of BMPs in relation to this phenotype. At 24 h post-fertilization (hpf), the expanded ICM of embryos injected with chd morpholino (MO) (Chd MO embryos) contained a monotonous population of hematopoietic progenitors. In situ hybridization showed that hematopoietic transcription factors were ubiquitously expressed in the ICM whereas vascular gene expression was confined to the periphery. BMP4 (but not BMP2b or 7) and smad5 mRNA were ectopically expressed in the Chd MO ICM. At 48 hpf, monocytic cells were evident in both the ICM and circulation of Chd MO but not WT embryos. While injection of BMP4 MO had no effect on WT hematopoiesis, co-injecting BMP4 with chd MOs significantly reduced ICM expansion. Microarray studies revealed a number of genes that were differentially expressed in Chd MO and WT embryos and their roles in hematopoiesis has yet to be determined. In conclusion, the expanded ICM in Chd MO embryos represented an expansion of embryonic hematopoiesis that was skewed towards a monocytic lineage. BMP4, but not BMP2b or 7, was involved in this process. The results provide ground for further research into the mechanisms of embryonic hematopoietic cell expansion. © 2004 Elsevier Inc. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio | en_HK |
dc.relation.ispartof | Developmental Biology | en_HK |
dc.subject | BMP | - |
dc.subject | Chordin | - |
dc.subject | Differentiation | - |
dc.subject | Gene expression | - |
dc.subject | Hematopoiesis | - |
dc.subject | Microarray | - |
dc.subject | Morpholino | - |
dc.subject | Zebrafish | - |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Bone Morphogenetic Protein 4 | en_HK |
dc.subject.mesh | Bone Morphogenetic Proteins - physiology | en_HK |
dc.subject.mesh | Cell Division | en_HK |
dc.subject.mesh | Dose-Response Relationship, Drug | en_HK |
dc.subject.mesh | Gene Expression Profiling | en_HK |
dc.subject.mesh | Glycoproteins - physiology | en_HK |
dc.subject.mesh | Hematopoiesis | en_HK |
dc.subject.mesh | Intercellular Signaling Peptides and Proteins - physiology | en_HK |
dc.subject.mesh | Oligonucleotide Array Sequence Analysis | en_HK |
dc.subject.mesh | Signal Transduction | en_HK |
dc.subject.mesh | Zebrafish - embryology | en_HK |
dc.subject.mesh | Zebrafish Proteins | en_HK |
dc.title | Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0012-1606&volume=277&issue=1&spage=235&epage=254&date=2005&atitle=Characterization+of+expanded+intermediate+cell+mass+in+zebrafish+chordin+morphant+embryos | en_HK |
dc.identifier.email | Leung, AYH:ayhleung@hku.hk | en_HK |
dc.identifier.email | Liang, R:rliang@hku.hk | en_HK |
dc.identifier.authority | Leung, AYH=rp00265 | en_HK |
dc.identifier.authority | Liang, R=rp00345 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.ydbio.2004.09.032 | en_HK |
dc.identifier.pmid | 15572152 | - |
dc.identifier.scopus | eid_2-s2.0-9644294510 | en_HK |
dc.identifier.hkuros | 99039 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-9644294510&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 277 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 235 | en_HK |
dc.identifier.epage | 254 | en_HK |
dc.identifier.isi | WOS:000225741200018 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Leung, AYH=7403012668 | en_HK |
dc.identifier.scopusauthorid | Mendenhall, EM=9741377300 | en_HK |
dc.identifier.scopusauthorid | Kwan, TTF=8776810800 | en_HK |
dc.identifier.scopusauthorid | Liang, R=26643224900 | en_HK |
dc.identifier.scopusauthorid | Eckfeldt, C=6507708270 | en_HK |
dc.identifier.scopusauthorid | Chen, E=7402316227 | en_HK |
dc.identifier.scopusauthorid | Hammerschmidt, M=7006962776 | en_HK |
dc.identifier.scopusauthorid | Grindley, S=8776811300 | en_HK |
dc.identifier.scopusauthorid | Ekker, SC=7003541150 | en_HK |
dc.identifier.scopusauthorid | Verfaillie, CM=7004524257 | en_HK |
dc.identifier.citeulike | 11790760 | - |
dc.identifier.issnl | 0012-1606 | - |