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Article: Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples

TitleComparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples
Authors
Kwok, HCWing, CYChiu, MPKit, MCSiu, YLKam, FLPoon, LLMPeiris, JSM
Issue Date2008
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2008, v. 46 n. 7, p. 2195-2199 How to Cite?
DOI: http://dx.doi.org/10.1128/JCM.00315-08
AbstractThe NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78962
ISSN
0095-1137
2021 Impact Factor: 11.677
2020 SCImago Journal Rankings: 2.349
ISI Accession Number ID
WOS:000258906800008
Funding AgencyGrant Number
Hong Kong Special Administrative Region, ChinaAoE/M-12/06
Control of Infectious Diseases of the Health, Welfare, and Food Bureau
Hong Kong Special Administrative Region, China
Funding Information:

We acknowledge funding from the University Grants Committee of the Hong Kong Special Administrative Region, China (project AoE/M-12/06), and the Research Fund for the Control of Infectious Diseases of the Health, Welfare, and Food Bureau of the Hong Kong Special Administrative Region, China.

References
References in Scopus
Grants
Control of Pandemic and Inter-pandemic Influenza

 

DC FieldValueLanguage
dc.contributor.authorKwok, HCen_HK
dc.contributor.authorWing, CYen_HK
dc.contributor.authorChiu, MPen_HK
dc.contributor.authorKit, MCen_HK
dc.contributor.authorSiu, YLen_HK
dc.contributor.authorKam, FLen_HK
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2010-09-06T07:48:55Z-
dc.date.available2010-09-06T07:48:55Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2008, v. 46 n. 7, p. 2195-2199en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78962-
dc.description.abstractThe NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination. Copyright © 2008, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.titleComparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samplesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=46&issue=7&spage=2195&epage=2199&date=2008&atitle=Comparison+of+the+NucliSens+easyMAG+and+Qiagen+BioRobot+9604+Nucleic+Acid+Extraction+Systems+for+Detection+of+RNA+and+DNA+Respiratory+Viruses+in+Nasopharyngeal+Aspirate+Samplesen_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/JCM.00315-08en_HK
dc.identifier.scopuseid_2-s2.0-49049090021en_HK
dc.identifier.hkuros148606en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49049090021&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2195en_HK
dc.identifier.epage2199en_HK
dc.identifier.isiWOS:000258906800008-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridKwok, HC=8617702800en_HK
dc.identifier.scopusauthoridWing, CY=25227043700en_HK
dc.identifier.scopusauthoridChiu, MP=25226678000en_HK
dc.identifier.scopusauthoridKit, MC=25226897500en_HK
dc.identifier.scopusauthoridSiu, YL=55254114000en_HK
dc.identifier.scopusauthoridKam, FL=25226884100en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.issnl0095-1137-

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