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- Publisher Website: 10.1016/j.femsle.2005.08.026
- Scopus: eid_2-s2.0-26844540494
- PMID: 16165318
- WOS: WOS:000232971800008
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Article: Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis
Title | Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis |
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Authors | |
Keywords | Expression Inducible Laribacter hongkongensis Shuttle vector |
Issue Date | 2005 |
Publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0378-1097&site=1 |
Citation | Fems Microbiology Letters, 2005, v. 252 n. 1, p. 57-65 How to Cite? |
Abstract | An Escherichia coli-Laribacter hongkongensis shuttle vector (pPW380) was constructed by ligating the 4701-bp EcoRI digested fragment of pHLHK8 to EcoRI digested pBK-CMV. An E. coli-L. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of pALC2084 to the 8897-bp fragment of pPW380, deletion of the green fluorescent protein gene, and insertion of a multiple cloning site. This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/79273 |
ISSN | 2023 Impact Factor: 2.2 2023 SCImago Journal Rankings: 0.513 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Woo, PCY | en_HK |
dc.contributor.author | Ma, SSL | en_HK |
dc.contributor.author | Teng, JLL | en_HK |
dc.contributor.author | Li, MWS | en_HK |
dc.contributor.author | Kao, RYT | en_HK |
dc.contributor.author | Lau, SKP | en_HK |
dc.contributor.author | Yuen, KY | en_HK |
dc.date.accessioned | 2010-09-06T07:52:41Z | - |
dc.date.available | 2010-09-06T07:52:41Z | - |
dc.date.issued | 2005 | en_HK |
dc.identifier.citation | Fems Microbiology Letters, 2005, v. 252 n. 1, p. 57-65 | en_HK |
dc.identifier.issn | 0378-1097 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/79273 | - |
dc.description.abstract | An Escherichia coli-Laribacter hongkongensis shuttle vector (pPW380) was constructed by ligating the 4701-bp EcoRI digested fragment of pHLHK8 to EcoRI digested pBK-CMV. An E. coli-L. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of pALC2084 to the 8897-bp fragment of pPW380, deletion of the green fluorescent protein gene, and insertion of a multiple cloning site. This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0378-1097&site=1 | en_HK |
dc.relation.ispartof | FEMS Microbiology Letters | en_HK |
dc.rights | F E M S Microbiology Letters. Copyright © Blackwell Publishing Ltd. | en_HK |
dc.subject | Expression | - |
dc.subject | Inducible | - |
dc.subject | Laribacter hongkongensis | - |
dc.subject | Shuttle vector | - |
dc.subject.mesh | Base Sequence | en_HK |
dc.subject.mesh | Escherichia coli - genetics - metabolism | en_HK |
dc.subject.mesh | Gastroenteritis - microbiology | en_HK |
dc.subject.mesh | Gene Expression Regulation, Bacterial | en_HK |
dc.subject.mesh | Genetic Vectors | en_HK |
dc.subject.mesh | Glutathione Transferase - genetics - metabolism | en_HK |
dc.subject.mesh | Green Fluorescent Proteins - genetics - metabolism | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Molecular Sequence Data | en_HK |
dc.subject.mesh | Neisseriaceae - genetics - isolation & purification - metabolism | en_HK |
dc.subject.mesh | Neisseriaceae Infections - microbiology | en_HK |
dc.subject.mesh | Plasmids | en_HK |
dc.subject.mesh | Sequence Analysis, DNA | en_HK |
dc.subject.mesh | Transformation, Genetic | en_HK |
dc.title | Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-1097&volume=252&spage=57&epage=65&date=2005&atitle=Construction+of+an+inducible+expression+shuttle+vector+for+Laribacter+hongkongensis,+a+novel+bacterium+associated+with+gastroenteritis | en_HK |
dc.identifier.email | Woo, PCY:pcywoo@hkucc.hku.hk | en_HK |
dc.identifier.email | Teng, JLL:llteng@hku.hk | en_HK |
dc.identifier.email | Kao, RYT:rytkao@hkucc.hku.hk | en_HK |
dc.identifier.email | Lau, SKP:skplau@hkucc.hku.hk | en_HK |
dc.identifier.email | Yuen, KY:kyyuen@hkucc.hku.hk | en_HK |
dc.identifier.authority | Woo, PCY=rp00430 | en_HK |
dc.identifier.authority | Teng, JLL=rp00277 | en_HK |
dc.identifier.authority | Kao, RYT=rp00481 | en_HK |
dc.identifier.authority | Lau, SKP=rp00486 | en_HK |
dc.identifier.authority | Yuen, KY=rp00366 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.femsle.2005.08.026 | en_HK |
dc.identifier.pmid | 16165318 | - |
dc.identifier.scopus | eid_2-s2.0-26844540494 | en_HK |
dc.identifier.hkuros | 114681 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-26844540494&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 252 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 57 | en_HK |
dc.identifier.epage | 65 | en_HK |
dc.identifier.isi | WOS:000232971800008 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Woo, PCY=7201801340 | en_HK |
dc.identifier.scopusauthorid | Ma, SSL=26424668200 | en_HK |
dc.identifier.scopusauthorid | Teng, JLL=7202560229 | en_HK |
dc.identifier.scopusauthorid | Li, MWS=8967745900 | en_HK |
dc.identifier.scopusauthorid | Kao, RYT=7101675499 | en_HK |
dc.identifier.scopusauthorid | Lau, SKP=7401596211 | en_HK |
dc.identifier.scopusauthorid | Yuen, KY=36078079100 | en_HK |
dc.identifier.issnl | 0378-1097 | - |