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- Publisher Website: 10.1002/pmic.200700757
- Scopus: eid_2-s2.0-43549083241
- PMID: 18398875
- WOS: WOS:000255720600017
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Article: Proteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells
Title | Proteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells |
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Authors | |
Keywords | AGS cell line Avian influenza H9N2 virus Cytokeratin Cytoplasmic actin Two-dimensional gel electrophoresis |
Issue Date | 2008 |
Publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics |
Citation | Proteomics, 2008, v. 8 n. 9, p. 1851-1858 How to Cite? |
Abstract | We present the first proteomic analysis on the cellular responses to avian influenza virus (H9N2) infection in a human cell line in different time courses in order to search for target proteins for viral pathogenesis/adaptation studies. By using 2-DE coupled with MALDI-TOF MS and nanoESI-MS/MS, we identified a set of differentially expressed cellular proteins, including cytoplasmic actin, cytokeratin, prohibitin, enoyl-CoA hydratase, peptide-prolyl cis-trans isomerase A (PPIase A), chloride intracellular channel protein 1, pyruvate dehydrogenase E1 component subunit beta, adenine phosphoribosyltransferase, guanine nucleotide-binding protein subunit beta, nucleoside diphosphate kinase A, elongation factor 1-beta and splicing factor, arginine/ serine rich 1. The most significant changes in different time courses were found in cytoplasmic actin and cytokeratin, both of which constituted the major components of cytoskeleton network in the cells. The obtained data suggested a possible role of the cytoskeleton during avian influenza virus infection of mammalian cells, which might help for better understanding of the dynamics of avian influenza virus and host interaction in mammalian cell setting. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA. |
Persistent Identifier | http://hdl.handle.net/10722/79310 |
ISSN | 2023 Impact Factor: 3.4 2023 SCImago Journal Rankings: 1.011 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liu, N | en_HK |
dc.contributor.author | Song, W | en_HK |
dc.contributor.author | Wang, P | en_HK |
dc.contributor.author | Lee, K | en_HK |
dc.contributor.author | Chan, W | en_HK |
dc.contributor.author | Chen, H | en_HK |
dc.contributor.author | Cai, Z | en_HK |
dc.date.accessioned | 2010-09-06T07:53:09Z | - |
dc.date.available | 2010-09-06T07:53:09Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Proteomics, 2008, v. 8 n. 9, p. 1851-1858 | en_HK |
dc.identifier.issn | 1615-9853 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/79310 | - |
dc.description.abstract | We present the first proteomic analysis on the cellular responses to avian influenza virus (H9N2) infection in a human cell line in different time courses in order to search for target proteins for viral pathogenesis/adaptation studies. By using 2-DE coupled with MALDI-TOF MS and nanoESI-MS/MS, we identified a set of differentially expressed cellular proteins, including cytoplasmic actin, cytokeratin, prohibitin, enoyl-CoA hydratase, peptide-prolyl cis-trans isomerase A (PPIase A), chloride intracellular channel protein 1, pyruvate dehydrogenase E1 component subunit beta, adenine phosphoribosyltransferase, guanine nucleotide-binding protein subunit beta, nucleoside diphosphate kinase A, elongation factor 1-beta and splicing factor, arginine/ serine rich 1. The most significant changes in different time courses were found in cytoplasmic actin and cytokeratin, both of which constituted the major components of cytoskeleton network in the cells. The obtained data suggested a possible role of the cytoskeleton during avian influenza virus infection of mammalian cells, which might help for better understanding of the dynamics of avian influenza virus and host interaction in mammalian cell setting. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics | en_HK |
dc.relation.ispartof | Proteomics | en_HK |
dc.subject | AGS cell line | - |
dc.subject | Avian influenza H9N2 virus | - |
dc.subject | Cytokeratin | - |
dc.subject | Cytoplasmic actin | - |
dc.subject | Two-dimensional gel electrophoresis | - |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Birds | en_HK |
dc.subject.mesh | Cytoplasm - metabolism | en_HK |
dc.subject.mesh | Cytoskeleton - metabolism | en_HK |
dc.subject.mesh | Gene Expression Profiling - methods | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Influenza A Virus, H9N2 Subtype - metabolism | en_HK |
dc.subject.mesh | Influenza in Birds - virology | en_HK |
dc.subject.mesh | Influenza, Human - virology | en_HK |
dc.subject.mesh | Keratins - metabolism | en_HK |
dc.subject.mesh | Mass Spectrometry - methods | en_HK |
dc.subject.mesh | Peptides - chemistry | en_HK |
dc.subject.mesh | Proteins - chemistry | en_HK |
dc.subject.mesh | Proteomics - methods | en_HK |
dc.subject.mesh | Spectrometry, Mass, Electrospray Ionization | en_HK |
dc.title | Proteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9853&volume=8&spage=1851&epage=1858&date=2008&atitle=Proteomics+analysis+of+differential+expression+of+cellular+proteins+in+response+to+avian+H9N2+virus+infection+in+human+cells. | en_HK |
dc.identifier.email | Chen, H:hlchen@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chen, H=rp00383 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/pmic.200700757 | en_HK |
dc.identifier.pmid | 18398875 | - |
dc.identifier.scopus | eid_2-s2.0-43549083241 | en_HK |
dc.identifier.hkuros | 147170 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-43549083241&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 8 | en_HK |
dc.identifier.issue | 9 | en_HK |
dc.identifier.spage | 1851 | en_HK |
dc.identifier.epage | 1858 | en_HK |
dc.identifier.isi | WOS:000255720600017 | - |
dc.publisher.place | Germany | en_HK |
dc.identifier.scopusauthorid | Liu, N=36079934800 | en_HK |
dc.identifier.scopusauthorid | Song, W=23490429800 | en_HK |
dc.identifier.scopusauthorid | Wang, P=13907209900 | en_HK |
dc.identifier.scopusauthorid | Lee, K=14060567400 | en_HK |
dc.identifier.scopusauthorid | Chan, W=7403917961 | en_HK |
dc.identifier.scopusauthorid | Chen, H=26643315400 | en_HK |
dc.identifier.scopusauthorid | Cai, Z=7402904946 | en_HK |
dc.identifier.issnl | 1615-9853 | - |