File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Differential responses of cord and adult blood-derived dendritic cells to dying cells

TitleDifferential responses of cord and adult blood-derived dendritic cells to dying cells
Authors
KeywordsApoptosis
Cord blood
Cytokines
Dendritic cell
Lymphoblastoid cells
Necrosis
Issue Date2005
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMM
Citation
Immunology, 2005, v. 116 n. 1, p. 13-20 How to Cite?
AbstractNormal turnover of body tissues yields apoptotic cells while infections cause tissue injuries and cell necrosis. The interaction of these dying cells with dendritic cells (DCs) may provide immunological instructions leading to either immune tolerance or activation. We hypothesize that neonatal and adult DCs differ in their responses to dying cells, thereby contributing to the observed differences in immune responses between neonates and adults. We compare the outcome of interaction of cord and adult blood-derived DCs with dying Epstein-Barr-virus-transformed lymphoblastoid cells (LCLs) and the responsiveness to lipopolysaccharide. While cord DCs were able to phagocytose both apoptotic and necrotic LCLs, the subsequent responses differed significantly from those of adult DCs. Interaction of adult DCs with necrotic but not early apoptotic LCLs resulted in high expression of DC costimulatory molecules (CD80/CD86) and activation markers (CD83), production of both proinflammatory and anti-inflammatory cytokines (tumour necrosis factor-α, interleukin-10), and strong T-cell-stimulating activities. In contrast, in response to either necrotic or apoptotic LCLs, cord DCs had minimal up-regulation of those DC functional markers, little cytokine production and poor stimulation on T-cell proliferation. In response to lipopolysaccharide, however, both adult and cord DCs produced comparable levels of tumour necrosis factor-α and interleukin-10, but only adult DCs produced interleukin-12(p70). Taken together, these results suggest that neonatal DCs generally favour immune tolerance with minimal activation and cytokine production, except in extremely dangerous situations, such as bacterial sepsis, when neonatal DCs may produce certain types of cytokines and stimulate T-cell proliferation. © 2005 Blackwell Publishing Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/79764
ISSN
2023 Impact Factor: 4.9
2023 SCImago Journal Rankings: 1.720
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, OHen_HK
dc.contributor.authorHuang, FPen_HK
dc.contributor.authorChiang, AKSen_HK
dc.date.accessioned2010-09-06T07:58:26Z-
dc.date.available2010-09-06T07:58:26Z-
dc.date.issued2005en_HK
dc.identifier.citationImmunology, 2005, v. 116 n. 1, p. 13-20en_HK
dc.identifier.issn0019-2805en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79764-
dc.description.abstractNormal turnover of body tissues yields apoptotic cells while infections cause tissue injuries and cell necrosis. The interaction of these dying cells with dendritic cells (DCs) may provide immunological instructions leading to either immune tolerance or activation. We hypothesize that neonatal and adult DCs differ in their responses to dying cells, thereby contributing to the observed differences in immune responses between neonates and adults. We compare the outcome of interaction of cord and adult blood-derived DCs with dying Epstein-Barr-virus-transformed lymphoblastoid cells (LCLs) and the responsiveness to lipopolysaccharide. While cord DCs were able to phagocytose both apoptotic and necrotic LCLs, the subsequent responses differed significantly from those of adult DCs. Interaction of adult DCs with necrotic but not early apoptotic LCLs resulted in high expression of DC costimulatory molecules (CD80/CD86) and activation markers (CD83), production of both proinflammatory and anti-inflammatory cytokines (tumour necrosis factor-α, interleukin-10), and strong T-cell-stimulating activities. In contrast, in response to either necrotic or apoptotic LCLs, cord DCs had minimal up-regulation of those DC functional markers, little cytokine production and poor stimulation on T-cell proliferation. In response to lipopolysaccharide, however, both adult and cord DCs produced comparable levels of tumour necrosis factor-α and interleukin-10, but only adult DCs produced interleukin-12(p70). Taken together, these results suggest that neonatal DCs generally favour immune tolerance with minimal activation and cytokine production, except in extremely dangerous situations, such as bacterial sepsis, when neonatal DCs may produce certain types of cytokines and stimulate T-cell proliferation. © 2005 Blackwell Publishing Ltd.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMMen_HK
dc.relation.ispartofImmunologyen_HK
dc.rightsImmunology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectApoptosisen_HK
dc.subjectCord blooden_HK
dc.subjectCytokinesen_HK
dc.subjectDendritic cellen_HK
dc.subjectLymphoblastoid cellsen_HK
dc.subjectNecrosisen_HK
dc.titleDifferential responses of cord and adult blood-derived dendritic cells to dying cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0019-2805&volume=116&spage=13&epage=20&date=2005&atitle=Differential+responses+of+cord+and+adult+blood-derived+dendritic+cells+to+dying+cellsen_HK
dc.identifier.emailChiang, AKS:chiangak@hkucc.hku.hken_HK
dc.identifier.authorityChiang, AKS=rp00403en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1365-2567.2005.02191.xen_HK
dc.identifier.pmid16108813-
dc.identifier.scopuseid_2-s2.0-23944478595en_HK
dc.identifier.hkuros109113en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-23944478595&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume116en_HK
dc.identifier.issue1en_HK
dc.identifier.spage13en_HK
dc.identifier.epage20en_HK
dc.identifier.isiWOS:000231224000002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, OH=8545473600en_HK
dc.identifier.scopusauthoridHuang, FP=35358818300en_HK
dc.identifier.scopusauthoridChiang, AKS=7101623534en_HK
dc.identifier.citeulike281743-
dc.identifier.issnl0019-2805-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats