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- Publisher Website: 10.1016/S0006-2952(01)00625-6
- Scopus: eid_2-s2.0-0035371005
- PMID: 11331080
- WOS: WOS:000168331700013
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Article: A mechanistic study of proliferation induced by Angelica sinensis in a normal gastric epithelial cell line
Title | A mechanistic study of proliferation induced by Angelica sinensis in a normal gastric epithelial cell line |
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Authors | |
Keywords | Angelica sinensis C-Myc Gastric epithelial cells ODC Polysaccharide Proliferation |
Issue Date | 2001 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/biochempharm |
Citation | Biochemical Pharmacology, 2001, v. 61 n. 11, p. 1439-1448 How to Cite? |
Abstract | It has been reported that an extract from Angelica sinensis mainly consisting of polysaccharides (95%) prevented ethanol- or indomethacin-induced gastric mucosal damage (Cho CH et al. Planta Med 2000;66:348-51). However, it is not known whether Angelica sinensis has a direct stimulatory effect on the healing of gastric mucosal lesions. To study the hypothesis that Angelica sinensis has a direct mucosal healing effect in rats and in isolated gastric epithelial cells, we assessed the wound repair in both animals and normal cell culture (RGM-1), as well as [ 3H]thymidine incorporation, ornithine decarboxylase (ODC) activity, and ODC protein and c-Myc protein expression after different treatments in RGM-1 cells. We found that Angelica sinensis crude extract (ASCE) dose-dependently enhanced gastric ulcer healing in rats and promoted wound repair in RGM-1 cells. It also significantly stimulated [ 3H]thymidine incorporation and ODC activity in RGM-1 cells in a concentration-dependent manner. ODC and c-Myc protein expression was also increased as a result of this process. DL-α-difluoromethyl-ornithine repressed the [ 3H]thymidine incorporation and ODC activity induced by ASCE. Pretreatment with c-Myc antisense oligodeoxynucleotides blocked the stimulatory action of ASCE on [ 3H]thymidine incorporation and ODC protein expression. These data suggest that ASCE has a direct mucosal healing effect on gastric epithelial cells, while ODC and c-Myc are closely associated with this effect. © 2001 Elsevier Science Inc. |
Persistent Identifier | http://hdl.handle.net/10722/80284 |
ISSN | 2023 Impact Factor: 5.3 2023 SCImago Journal Rankings: 1.365 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Ye, YN | en_HK |
dc.contributor.author | Liu, ESL | en_HK |
dc.contributor.author | Shin, VY | en_HK |
dc.contributor.author | Koo, MWL | en_HK |
dc.contributor.author | Li, Y | en_HK |
dc.contributor.author | Wei, EQ | en_HK |
dc.contributor.author | Matsui, H | en_HK |
dc.contributor.author | Cho, CH | en_HK |
dc.date.accessioned | 2010-09-06T08:04:36Z | - |
dc.date.available | 2010-09-06T08:04:36Z | - |
dc.date.issued | 2001 | en_HK |
dc.identifier.citation | Biochemical Pharmacology, 2001, v. 61 n. 11, p. 1439-1448 | en_HK |
dc.identifier.issn | 0006-2952 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/80284 | - |
dc.description.abstract | It has been reported that an extract from Angelica sinensis mainly consisting of polysaccharides (95%) prevented ethanol- or indomethacin-induced gastric mucosal damage (Cho CH et al. Planta Med 2000;66:348-51). However, it is not known whether Angelica sinensis has a direct stimulatory effect on the healing of gastric mucosal lesions. To study the hypothesis that Angelica sinensis has a direct mucosal healing effect in rats and in isolated gastric epithelial cells, we assessed the wound repair in both animals and normal cell culture (RGM-1), as well as [ 3H]thymidine incorporation, ornithine decarboxylase (ODC) activity, and ODC protein and c-Myc protein expression after different treatments in RGM-1 cells. We found that Angelica sinensis crude extract (ASCE) dose-dependently enhanced gastric ulcer healing in rats and promoted wound repair in RGM-1 cells. It also significantly stimulated [ 3H]thymidine incorporation and ODC activity in RGM-1 cells in a concentration-dependent manner. ODC and c-Myc protein expression was also increased as a result of this process. DL-α-difluoromethyl-ornithine repressed the [ 3H]thymidine incorporation and ODC activity induced by ASCE. Pretreatment with c-Myc antisense oligodeoxynucleotides blocked the stimulatory action of ASCE on [ 3H]thymidine incorporation and ODC protein expression. These data suggest that ASCE has a direct mucosal healing effect on gastric epithelial cells, while ODC and c-Myc are closely associated with this effect. © 2001 Elsevier Science Inc. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/biochempharm | en_HK |
dc.relation.ispartof | Biochemical Pharmacology | en_HK |
dc.rights | Biochemical Pharmacology. Copyright © Elsevier Inc. | en_HK |
dc.subject | Angelica sinensis | en_HK |
dc.subject | C-Myc | en_HK |
dc.subject | Gastric epithelial cells | en_HK |
dc.subject | ODC | en_HK |
dc.subject | Polysaccharide | en_HK |
dc.subject | Proliferation | en_HK |
dc.title | A mechanistic study of proliferation induced by Angelica sinensis in a normal gastric epithelial cell line | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-2952&volume=61&spage=1439&epage=1448&date=2001&atitle=A+mechanistic+study+of+proliferation+induced+by+Angelica+sinensis+in+a+normal+gastric+epithelial+cell+line | en_HK |
dc.identifier.email | Koo, MWL: wlkoo@hku.hk | en_HK |
dc.identifier.authority | Koo, MWL=rp00233 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/S0006-2952(01)00625-6 | en_HK |
dc.identifier.pmid | 11331080 | - |
dc.identifier.scopus | eid_2-s2.0-0035371005 | en_HK |
dc.identifier.hkuros | 58930 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0035371005&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 61 | en_HK |
dc.identifier.issue | 11 | en_HK |
dc.identifier.spage | 1439 | en_HK |
dc.identifier.epage | 1448 | en_HK |
dc.identifier.isi | WOS:000168331700013 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Ye, YN=7401627402 | en_HK |
dc.identifier.scopusauthorid | Liu, ESL=7202240071 | en_HK |
dc.identifier.scopusauthorid | Shin, VY=7003491170 | en_HK |
dc.identifier.scopusauthorid | Koo, MWL=7004550899 | en_HK |
dc.identifier.scopusauthorid | Li, Y=54970757000 | en_HK |
dc.identifier.scopusauthorid | Wei, EQ=7102156300 | en_HK |
dc.identifier.scopusauthorid | Matsui, H=7401527136 | en_HK |
dc.identifier.scopusauthorid | Cho, CH=7403100461 | en_HK |
dc.identifier.issnl | 0006-2952 | - |