File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/S0006-8993(00)02913-9
- Scopus: eid_2-s2.0-0034711560
- PMID: 11082490
- WOS: WOS:000165749200010
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Co-localization of NMDA receptors and AMPA receptors in neurons of the vestibular nuclei of rats
Title | Co-localization of NMDA receptors and AMPA receptors in neurons of the vestibular nuclei of rats |
---|---|
Authors | |
Keywords | AMPA receptor Co-localization Immunocytochemistry NMDA receptor Vestibular nucleus |
Issue Date | 2000 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres |
Citation | Brain Research, 2000, v. 884 n. 1-2, p. 87-97 How to Cite? |
Abstract | We are interested in studying the co-localization of NMDA glutamate receptor subunits (NR1, NR2A/B) and AMPA glutamate receptor subunits (GluR1, GluR2, GluR2/3 and GluR4) in individual neurons of the rat vestibular nuclei. Immunoreactivity for NR1, NR2A/B, GluR1, GluR2, GluR2/3 and GluR4 was found in the somata and dendrites of neurons in the four major subdivisions (superior, medial, lateral, and spinal vestibular nuclei) and in two minor groups (groups x and y) of the vestibular nuclei. Double immunofluorescence showed that all the NR1-containing neurons exhibited NR2A/B immunoreactivity, indicating that native NMDA receptors are composed of NR1 and NR2A/B in a hetero-oligomeric configuration. Co-expression of NMDA receptor subunits and AMPA receptor subunits was demonstrated by double labeling of NR1/GluR1, NR1/GluR2/3, NR1/GluR4 and NR2A/B/GluR2 in individual vestibular nuclear neurons. All NR1-containing neurons expressed GluR2/3 immunoreactivity, and all NR2A/B-containing neurons expressed GluR2 immunoreactivity. However, only about 52% of NR1-immunoreactive neurons exhibited GluR1 immunoreactivity and 46% of NR1-containing neurons showed GluR4 immunoreactivity. The present data reveal that NMDA receptors are co-localized with variants of AMPA receptors in a large proportion of vestibular nuclear neurons. These results suggest that cross-modulation between NMDA receptors and AMPA receptors may occur in individual neurons of the vestibular nuclei during glutamate-mediated excitatory neurotransmission and may in turn contribute to synaptic plasticity within the vestibular nuclei. Copyright (C) 2000 Elsevier Science B.V. |
Persistent Identifier | http://hdl.handle.net/10722/81274 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.832 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, LW | en_HK |
dc.contributor.author | Yung, KKL | en_HK |
dc.contributor.author | Chan, YS | en_HK |
dc.date.accessioned | 2010-09-06T08:15:48Z | - |
dc.date.available | 2010-09-06T08:15:48Z | - |
dc.date.issued | 2000 | en_HK |
dc.identifier.citation | Brain Research, 2000, v. 884 n. 1-2, p. 87-97 | en_HK |
dc.identifier.issn | 0006-8993 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/81274 | - |
dc.description.abstract | We are interested in studying the co-localization of NMDA glutamate receptor subunits (NR1, NR2A/B) and AMPA glutamate receptor subunits (GluR1, GluR2, GluR2/3 and GluR4) in individual neurons of the rat vestibular nuclei. Immunoreactivity for NR1, NR2A/B, GluR1, GluR2, GluR2/3 and GluR4 was found in the somata and dendrites of neurons in the four major subdivisions (superior, medial, lateral, and spinal vestibular nuclei) and in two minor groups (groups x and y) of the vestibular nuclei. Double immunofluorescence showed that all the NR1-containing neurons exhibited NR2A/B immunoreactivity, indicating that native NMDA receptors are composed of NR1 and NR2A/B in a hetero-oligomeric configuration. Co-expression of NMDA receptor subunits and AMPA receptor subunits was demonstrated by double labeling of NR1/GluR1, NR1/GluR2/3, NR1/GluR4 and NR2A/B/GluR2 in individual vestibular nuclear neurons. All NR1-containing neurons expressed GluR2/3 immunoreactivity, and all NR2A/B-containing neurons expressed GluR2 immunoreactivity. However, only about 52% of NR1-immunoreactive neurons exhibited GluR1 immunoreactivity and 46% of NR1-containing neurons showed GluR4 immunoreactivity. The present data reveal that NMDA receptors are co-localized with variants of AMPA receptors in a large proportion of vestibular nuclear neurons. These results suggest that cross-modulation between NMDA receptors and AMPA receptors may occur in individual neurons of the vestibular nuclei during glutamate-mediated excitatory neurotransmission and may in turn contribute to synaptic plasticity within the vestibular nuclei. Copyright (C) 2000 Elsevier Science B.V. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres | en_HK |
dc.relation.ispartof | Brain Research | en_HK |
dc.rights | Brain Research. Copyright © Elsevier BV. | en_HK |
dc.subject | AMPA receptor | en_HK |
dc.subject | Co-localization | en_HK |
dc.subject | Immunocytochemistry | en_HK |
dc.subject | NMDA receptor | en_HK |
dc.subject | Vestibular nucleus | en_HK |
dc.title | Co-localization of NMDA receptors and AMPA receptors in neurons of the vestibular nuclei of rats | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=884&spage=87&epage=97&date=2000&atitle=Co-localization+of+NMDA+receptors+and+AMPA+receptors+in+neurons+of+the+vestibular+nucleus+of+rats | en_HK |
dc.identifier.email | Chan, YS: yschan@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chan, YS=rp00318 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/S0006-8993(00)02913-9 | en_HK |
dc.identifier.pmid | 11082490 | - |
dc.identifier.scopus | eid_2-s2.0-0034711560 | en_HK |
dc.identifier.hkuros | 60096 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034711560&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 884 | en_HK |
dc.identifier.issue | 1-2 | en_HK |
dc.identifier.spage | 87 | en_HK |
dc.identifier.epage | 97 | en_HK |
dc.identifier.isi | WOS:000165749200010 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Chen, LW=7409444941 | en_HK |
dc.identifier.scopusauthorid | Yung, KKL=13605496000 | en_HK |
dc.identifier.scopusauthorid | Chan, YS=7403676627 | en_HK |
dc.identifier.issnl | 0006-8993 | - |