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Article: Significant up-regulation of nestin protein in the neostriatum of MPTP-treated mice: Are the striatal astrocytes regionally activated after systemic MPTP administration?

TitleSignificant up-regulation of nestin protein in the neostriatum of MPTP-treated mice: Are the striatal astrocytes regionally activated after systemic MPTP administration?
Authors
KeywordsCaudate putamen
Glia
Mouse
MPTP
Nestin protein
Parkinsonism
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2002, v. 925 n. 1, p. 9-17 How to Cite?
AbstractWe are interested in the possible role of central glial cells in pathogenesis of Parkinson's disease of mammals. Parkinsonism model was induced by systemic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration, and the reactive glial cells were examined by immunocytochemical visualization of nestin protein in the brains and spinal cords of C57 mice. Abundant nestin-like immunoreactivity was predominately found in the caudate putamen of MPTP-treated mice and about 481-fold of nestin-like immunoreactive cells increased compared with that of control animals, indicating that significant up-regulation of nestin protein occurred in these regions. Majority of nestin-like immunoreactive cells characterized with astrocytic profiles of multiple, radical and hypotrophic processes, and showed a distribution and dynamic patterns similar to that of glial fibrillary acid protein (GFAP)-immunoreactive cells in the caudate putamen. Double immunofluorescence confirmed that 100% of nestin-like immunoreactive cells exhibited GFAP-immunoreactivity while nestin/GFAP double-labeled cells constituted about 84% of total GFAP-immunoreactive cells in the caudate putamen, indicating these nestin-like immunoreactive cells belong to a reactive population of the astrocytes. On the other hand, no obvious changes of nestin- or GFAP-like immunoreactivities were detected in the globus pallidus, the substantia nigra and the ventral tegmental area after MPTP-treatment. The results have provided morphological evidence for the regional activation of astrocytic glial cells following systemic MPTP administration, suggesting that a large population of reactive striatal astrocytes might play an important role in initial pathogenesis or acute stage of Parkinson's disease in mammals. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/81332
ISSN
2023 Impact Factor: 2.7
2023 SCImago Journal Rankings: 0.832
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, LWen_HK
dc.contributor.authorWei, LCen_HK
dc.contributor.authorQiu, Yen_HK
dc.contributor.authorLiu, HLen_HK
dc.contributor.authorRao, ZRen_HK
dc.contributor.authorJu, Gen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2010-09-06T08:16:27Z-
dc.date.available2010-09-06T08:16:27Z-
dc.date.issued2002en_HK
dc.identifier.citationBrain Research, 2002, v. 925 n. 1, p. 9-17en_HK
dc.identifier.issn0006-8993en_HK
dc.identifier.urihttp://hdl.handle.net/10722/81332-
dc.description.abstractWe are interested in the possible role of central glial cells in pathogenesis of Parkinson's disease of mammals. Parkinsonism model was induced by systemic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration, and the reactive glial cells were examined by immunocytochemical visualization of nestin protein in the brains and spinal cords of C57 mice. Abundant nestin-like immunoreactivity was predominately found in the caudate putamen of MPTP-treated mice and about 481-fold of nestin-like immunoreactive cells increased compared with that of control animals, indicating that significant up-regulation of nestin protein occurred in these regions. Majority of nestin-like immunoreactive cells characterized with astrocytic profiles of multiple, radical and hypotrophic processes, and showed a distribution and dynamic patterns similar to that of glial fibrillary acid protein (GFAP)-immunoreactive cells in the caudate putamen. Double immunofluorescence confirmed that 100% of nestin-like immunoreactive cells exhibited GFAP-immunoreactivity while nestin/GFAP double-labeled cells constituted about 84% of total GFAP-immunoreactive cells in the caudate putamen, indicating these nestin-like immunoreactive cells belong to a reactive population of the astrocytes. On the other hand, no obvious changes of nestin- or GFAP-like immunoreactivities were detected in the globus pallidus, the substantia nigra and the ventral tegmental area after MPTP-treatment. The results have provided morphological evidence for the regional activation of astrocytic glial cells following systemic MPTP administration, suggesting that a large population of reactive striatal astrocytes might play an important role in initial pathogenesis or acute stage of Parkinson's disease in mammals. © 2002 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_HK
dc.relation.ispartofBrain Researchen_HK
dc.rightsBrain Research. Copyright © Elsevier BV.en_HK
dc.subjectCaudate putamen-
dc.subjectGlia-
dc.subjectMouse-
dc.subjectMPTP-
dc.subjectNestin protein-
dc.subjectParkinsonism-
dc.subject.mesh1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - pharmacologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAstrocytes - chemistry - metabolismen_HK
dc.subject.meshDopamine Agents - pharmacologyen_HK
dc.subject.meshGlial Fibrillary Acidic Protein - analysisen_HK
dc.subject.meshIntermediate Filament Proteins - analysis - metabolismen_HK
dc.subject.meshMPTP Poisoning - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshNeostriatum - cytology - metabolismen_HK
dc.subject.meshNerve Tissue Proteinsen_HK
dc.subject.meshSpinal Cord - cytology - metabolismen_HK
dc.titleSignificant up-regulation of nestin protein in the neostriatum of MPTP-treated mice: Are the striatal astrocytes regionally activated after systemic MPTP administration?en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=925&spage=9&epage=17&date=2002&atitle=Significant+up-regulation+of+nestin+protein+in+the+neostriatum+of+MPTP-treated+mice:+Are+the+striatal+astrocytes+regionally+activated+after+systemic+MPTP+administration?en_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-8993(01)03253-Xen_HK
dc.identifier.pmid11755896-
dc.identifier.scopuseid_2-s2.0-0037126959en_HK
dc.identifier.hkuros72004en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037126959&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume925en_HK
dc.identifier.issue1en_HK
dc.identifier.spage9en_HK
dc.identifier.epage17en_HK
dc.identifier.isiWOS:000173275100002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, LW=7409444941en_HK
dc.identifier.scopusauthoridWei, LC=7402951429en_HK
dc.identifier.scopusauthoridQiu, Y=7403279738en_HK
dc.identifier.scopusauthoridLiu, HL=36066506000en_HK
dc.identifier.scopusauthoridRao, ZR=7102549067en_HK
dc.identifier.scopusauthoridJu, G=26642903700en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.issnl0006-8993-

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