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- Publisher Website: 10.1046/j.1365-2168.1999.01241.x
- Scopus: eid_2-s2.0-0032695070
- PMID: 10583290
- WOS: WOS:000083589900013
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Article: Role of nitric oxide in intestinal ischaemia-reperfusion injury studied using electron paramagnetic resonance
Title | Role of nitric oxide in intestinal ischaemia-reperfusion injury studied using electron paramagnetic resonance |
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Authors | |
Issue Date | 1999 |
Publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.bjs.co.uk |
Citation | British Journal Of Surgery, 1999, v. 86 n. 11, p. 1427-1432 How to Cite? |
Abstract | Background: The Role Of Nitric Oxide In Intestinal Ischaemia-Reperfusion (I/R) Remains Poorly Defined, Partly Because Of Difficulty In Detecting The Nitric Oxide Free Radical. In This Study Nitric Oxide Production Was Assessed During Intestinal I/R By Direct Measurement Using Electron Paramagnetic Resonance (Epr), And The Production Of Nitric Oxide In Jejunum And Ileum Was Correlated With Their Different Abilities To Resist I/R Injury. Methods: Rats Were Given An Electron Spin Trapper (Diethyldithiocarbamate/Ferrous Citrate) By Intraperitoneal Injection. Thirty-Six Segments Each Of Jejunum And Ileum Were Subjected To 15-90 Min Of Ischaemia And 25 Min Of Reperfusion. Tissue Samples Were Analysed For Epr Signals Using A Spectrometer. Results: Mean (S.D.) Basal Nitric Oxide Level Was Significantly Higher In Ileum (3.39 (1.42) Units) Than Jejunum (0.65 (0.05) Units) (P = 0.0005). Increasing Ischaemic Times In The Ileum Resulted In Decreasing Nitric Oxide Levels (85, 32 And 13 Per Cent Of Basal Level At 30, 60 And 90 Min Respectively); Reperfusion Resulted In Further Nitric Oxide Reduction (Mean Decrease 26 Per Cent). Severe (Grade 3) Histological Damage Was Observed In Low Nitric Oxide States (After 15 Min Of I/R In Jejunum, 60 Min Of I/R In Ileum). Conclusion: Nitric Oxide Can Be Measured In Intestinal Tissues Directly By Epr. The Findings Support A Protective Role For Nitric Oxide In I/R, And Offer An Explanation For The Greater Resistance To I/R Of Ileum. |
Persistent Identifier | http://hdl.handle.net/10722/83506 |
ISSN | 2023 Impact Factor: 8.6 2023 SCImago Journal Rankings: 2.148 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Chan, KL | en_HK |
dc.contributor.author | Zhang, X | en_HK |
dc.contributor.author | Fung, PCW | en_HK |
dc.contributor.author | Guo, WH | en_HK |
dc.contributor.author | Tam, PKH | en_HK |
dc.date.accessioned | 2010-09-06T08:41:51Z | - |
dc.date.available | 2010-09-06T08:41:51Z | - |
dc.date.issued | 1999 | en_HK |
dc.identifier.citation | British Journal Of Surgery, 1999, v. 86 n. 11, p. 1427-1432 | en_US |
dc.identifier.issn | 0007-1323 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/83506 | - |
dc.description.abstract | Background: The Role Of Nitric Oxide In Intestinal Ischaemia-Reperfusion (I/R) Remains Poorly Defined, Partly Because Of Difficulty In Detecting The Nitric Oxide Free Radical. In This Study Nitric Oxide Production Was Assessed During Intestinal I/R By Direct Measurement Using Electron Paramagnetic Resonance (Epr), And The Production Of Nitric Oxide In Jejunum And Ileum Was Correlated With Their Different Abilities To Resist I/R Injury. Methods: Rats Were Given An Electron Spin Trapper (Diethyldithiocarbamate/Ferrous Citrate) By Intraperitoneal Injection. Thirty-Six Segments Each Of Jejunum And Ileum Were Subjected To 15-90 Min Of Ischaemia And 25 Min Of Reperfusion. Tissue Samples Were Analysed For Epr Signals Using A Spectrometer. Results: Mean (S.D.) Basal Nitric Oxide Level Was Significantly Higher In Ileum (3.39 (1.42) Units) Than Jejunum (0.65 (0.05) Units) (P = 0.0005). Increasing Ischaemic Times In The Ileum Resulted In Decreasing Nitric Oxide Levels (85, 32 And 13 Per Cent Of Basal Level At 30, 60 And 90 Min Respectively); Reperfusion Resulted In Further Nitric Oxide Reduction (Mean Decrease 26 Per Cent). Severe (Grade 3) Histological Damage Was Observed In Low Nitric Oxide States (After 15 Min Of I/R In Jejunum, 60 Min Of I/R In Ileum). Conclusion: Nitric Oxide Can Be Measured In Intestinal Tissues Directly By Epr. The Findings Support A Protective Role For Nitric Oxide In I/R, And Offer An Explanation For The Greater Resistance To I/R Of Ileum. | en_US |
dc.language | eng | en_HK |
dc.publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www.bjs.co.uk | en_HK |
dc.relation.ispartof | British Journal of Surgery | en_HK |
dc.rights | British Journal of Surgery. Copyright © John Wiley & Sons Ltd. | en_HK |
dc.title | Role of nitric oxide in intestinal ischaemia-reperfusion injury studied using electron paramagnetic resonance | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1323&volume=86&spage=1427&epage=1432&date=1999&atitle=Role+of+nitric+oxide+in+intestinal+ischaemia-reperfusion+injury+studied+using+electron+paramagnetic+resonance | en_HK |
dc.identifier.email | Chan, KL: klchan@HKUCC.hku.hk | en_HK |
dc.identifier.email | Zhang, X: ynzhangx@sohu.com | en_HK |
dc.identifier.email | Fung, PCW: hrspfcw@hku.hk | en_HK |
dc.identifier.email | Tam, PKH: paultam@hkucc.hku.hk | en_HK |
dc.identifier.authority | Tam, PKH=rp00060 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1046/j.1365-2168.1999.01241.x | en_US |
dc.identifier.pmid | 10583290 | - |
dc.identifier.scopus | eid_2-s2.0-0032695070 | en_US |
dc.identifier.hkuros | 47290 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032695070&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 86 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.spage | 1427 | en_US |
dc.identifier.epage | 1432 | en_US |
dc.identifier.isi | WOS:000083589900013 | - |
dc.identifier.issnl | 0007-1323 | - |