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- Publisher Website: 10.1161/01.CIR.0000066911.03770.8D
- Scopus: eid_2-s2.0-0037674918
- PMID: 12732603
- WOS: WOS:000183165900022
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Article: Prevention of chronic deterioration of heart allograft by recombinant adeno-associated virus-mediated heme oxygenase-1 gene transfer
Title | Prevention of chronic deterioration of heart allograft by recombinant adeno-associated virus-mediated heme oxygenase-1 gene transfer |
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Authors | |
Keywords | Arteriosclerosis Gene therapy Grafting Remodeling Transplantation |
Issue Date | 2003 |
Publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.org |
Citation | Circulation, 2003, v. 107 n. 20, p. 2623-2629 How to Cite? |
Abstract | Background - Allograft deterioration is the major obstacle to organ transplantation as a long-term treatment of end-stage heart failure. In this study, we transduced the antioxidant gene, heme oxygenase-1 (HO-1), to heart grafts using a recombinant adeno-associated viral vector (rAAV) in a rat heart transplantation model and investigated its potentiality in prevention of chronic graft deterioration. Methods and Results - rAAV/HO-1 was administered to heart grafts through the coronary arteries during cold preservation. We investigated the expression patterns and activities of transgene, graft survival, graft histomorphology, and relevance of HO-1 expression on graft survival and chronic graft deterioration by itself. Long-term allograft survival can be achieved by rAAV/HO-1-mediated stable transgene expression. The development of graft arteriosclerosis and interstitial fibrosis was prevented in rAAV/HO-1-transduced allografts on day 100. rAAV/HO-1-mediated long-term graft protection was accompanied by remarkable downregulation of the intragraft mRNA level of macrophage migration inhibitory factor, tumor necrosis factor-α, and transforming growth factor-β1. Blockage of HO activities by zinc protoporphyrin IX at different posttransplant phases showed that the stable expression of HO-1 is a prerequisite for both survival of grafts and prevention of graft arteriosclerosis. Conclusions - rAAV/HO-1 gene transfer represents a novel therapeutic approach to prevent chronic allograft deterioration in clinical heart transplantation. |
Persistent Identifier | http://hdl.handle.net/10722/84520 |
ISSN | 2023 Impact Factor: 35.5 2023 SCImago Journal Rankings: 8.415 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tsui, TY | en_HK |
dc.contributor.author | Wu, X | en_HK |
dc.contributor.author | Lau, CK | en_HK |
dc.contributor.author | Ho, DWY | en_HK |
dc.contributor.author | Xu, T | en_HK |
dc.contributor.author | Siu, YT | en_HK |
dc.contributor.author | Fan, ST | en_HK |
dc.date.accessioned | 2010-09-06T08:53:55Z | - |
dc.date.available | 2010-09-06T08:53:55Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | Circulation, 2003, v. 107 n. 20, p. 2623-2629 | en_HK |
dc.identifier.issn | 0009-7322 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/84520 | - |
dc.description.abstract | Background - Allograft deterioration is the major obstacle to organ transplantation as a long-term treatment of end-stage heart failure. In this study, we transduced the antioxidant gene, heme oxygenase-1 (HO-1), to heart grafts using a recombinant adeno-associated viral vector (rAAV) in a rat heart transplantation model and investigated its potentiality in prevention of chronic graft deterioration. Methods and Results - rAAV/HO-1 was administered to heart grafts through the coronary arteries during cold preservation. We investigated the expression patterns and activities of transgene, graft survival, graft histomorphology, and relevance of HO-1 expression on graft survival and chronic graft deterioration by itself. Long-term allograft survival can be achieved by rAAV/HO-1-mediated stable transgene expression. The development of graft arteriosclerosis and interstitial fibrosis was prevented in rAAV/HO-1-transduced allografts on day 100. rAAV/HO-1-mediated long-term graft protection was accompanied by remarkable downregulation of the intragraft mRNA level of macrophage migration inhibitory factor, tumor necrosis factor-α, and transforming growth factor-β1. Blockage of HO activities by zinc protoporphyrin IX at different posttransplant phases showed that the stable expression of HO-1 is a prerequisite for both survival of grafts and prevention of graft arteriosclerosis. Conclusions - rAAV/HO-1 gene transfer represents a novel therapeutic approach to prevent chronic allograft deterioration in clinical heart transplantation. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.org | en_HK |
dc.relation.ispartof | Circulation | en_HK |
dc.rights | Circulation. Copyright © Lippincott Williams & Wilkins. | en_HK |
dc.subject | Arteriosclerosis | en_HK |
dc.subject | Gene therapy | en_HK |
dc.subject | Grafting | en_HK |
dc.subject | Remodeling | en_HK |
dc.subject | Transplantation | en_HK |
dc.title | Prevention of chronic deterioration of heart allograft by recombinant adeno-associated virus-mediated heme oxygenase-1 gene transfer | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0009-7322&volume=107&spage=2623&epage=2629&date=2003&atitle=Prevention+of+chronic+deterioration+of+heart+allograft+by+recombinant+adeno-associated+virus-mediated+heme+oxygenase-1+gene+transfer | en_HK |
dc.identifier.email | Fan, ST: stfan@hku.hk | en_HK |
dc.identifier.authority | Fan, ST=rp00355 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1161/01.CIR.0000066911.03770.8D | - |
dc.identifier.pmid | 12732603 | - |
dc.identifier.scopus | eid_2-s2.0-0037674918 | en_HK |
dc.identifier.hkuros | 79153 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037674918&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 107 | en_HK |
dc.identifier.issue | 20 | en_HK |
dc.identifier.spage | 2623 | en_HK |
dc.identifier.epage | 2629 | en_HK |
dc.identifier.isi | WOS:000183165900022 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Tsui, TY=7006622455 | en_HK |
dc.identifier.scopusauthorid | Wu, X=7408231534 | en_HK |
dc.identifier.scopusauthorid | Lau, CK=7401968442 | en_HK |
dc.identifier.scopusauthorid | Ho, DWY=7402971906 | en_HK |
dc.identifier.scopusauthorid | Xu, T=7401627167 | en_HK |
dc.identifier.scopusauthorid | Siu, YT=8953557400 | en_HK |
dc.identifier.scopusauthorid | Fan, ST=7402678224 | en_HK |
dc.identifier.issnl | 0009-7322 | - |