File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification of a proglucagon cDNA from Rana tigrina rugulosa that encodes two GLP-1s and that is alternatively spliced in a tissue-specific manner

TitleIdentification of a proglucagon cDNA from Rana tigrina rugulosa that encodes two GLP-1s and that is alternatively spliced in a tissue-specific manner
Authors
KeywordsAlternative mRNA splicing
GLP-1
GLP-2
Glucagon
Proglucagon cDNA
Issue Date2001
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 2001, v. 124 n. 2, p. 144-151 How to Cite?
AbstractGlucagon plays a pivotal role in the regulation of metabolism. A glucagon receptor has been previously characterized in the frog, Rana tigrina rugulosa, and the frog and human glucagon receptors have been shown to possess similar binding affinities toward human glucagon. To study the structural evolution of glucagon peptide and its receptor in vertebrates, in the current study, a proglucagon cDNA from the same frog species was cloned. Interestingly, in contrast to the mammalian proglucagons that contain only one GLP-1 peptide, the frog proglucagon cDNA encodes two GLP-1 peptides (GLP-1A and GLP-1B) in addition to a glucagon peptide and a glucagon-like peptide 2 (GLP-2). By reverse transcriptase-PCR (RT-PCR) analysis, the proglucagon gene expression was widely detected in the brain, colon, small intestine, liver, lung, and pancreas, suggesting that the proglucagon-derived peptides have diverse functions in frogs. Moreover, tissue-specific alternative mRNA splicing was observed in the brain, colon, and pancreas. In these tissues, proglucagon transcripts with a 135 bp in frame deletion encoding GLP-1A were found. This splicing event in R. tigrina rugulosa is novel because it deletes a GLP-1 encoding sequence instead of the GLP-2 observed in other vertebrates. These findings should enhance understanding of the proglucagon evolution, structure, and expression in vertebrates. © 2001 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/84864
ISSN
2023 Impact Factor: 2.1
2023 SCImago Journal Rankings: 0.616
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, CMen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:58:00Z-
dc.date.available2010-09-06T08:58:00Z-
dc.date.issued2001en_HK
dc.identifier.citationGeneral And Comparative Endocrinology, 2001, v. 124 n. 2, p. 144-151en_HK
dc.identifier.issn0016-6480en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84864-
dc.description.abstractGlucagon plays a pivotal role in the regulation of metabolism. A glucagon receptor has been previously characterized in the frog, Rana tigrina rugulosa, and the frog and human glucagon receptors have been shown to possess similar binding affinities toward human glucagon. To study the structural evolution of glucagon peptide and its receptor in vertebrates, in the current study, a proglucagon cDNA from the same frog species was cloned. Interestingly, in contrast to the mammalian proglucagons that contain only one GLP-1 peptide, the frog proglucagon cDNA encodes two GLP-1 peptides (GLP-1A and GLP-1B) in addition to a glucagon peptide and a glucagon-like peptide 2 (GLP-2). By reverse transcriptase-PCR (RT-PCR) analysis, the proglucagon gene expression was widely detected in the brain, colon, small intestine, liver, lung, and pancreas, suggesting that the proglucagon-derived peptides have diverse functions in frogs. Moreover, tissue-specific alternative mRNA splicing was observed in the brain, colon, and pancreas. In these tissues, proglucagon transcripts with a 135 bp in frame deletion encoding GLP-1A were found. This splicing event in R. tigrina rugulosa is novel because it deletes a GLP-1 encoding sequence instead of the GLP-2 observed in other vertebrates. These findings should enhance understanding of the proglucagon evolution, structure, and expression in vertebrates. © 2001 Academic Press.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_HK
dc.relation.ispartofGeneral and Comparative Endocrinologyen_HK
dc.subjectAlternative mRNA splicingen_HK
dc.subjectGLP-1en_HK
dc.subjectGLP-2en_HK
dc.subjectGlucagonen_HK
dc.subjectProglucagon cDNAen_HK
dc.titleIdentification of a proglucagon cDNA from Rana tigrina rugulosa that encodes two GLP-1s and that is alternatively spliced in a tissue-specific manneren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-6480&volume=124&spage=144&epage=151&date=2001&atitle=Identification+of+a+Proglucagon+cDNA+from+Rana+tigrina+rugulosa+That+Encodes+Two+GLP-1s+and+That+Is+Alternatively+Spliced+in+a+Tissue-Specific+Manneren_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/gcen.2001.7697en_HK
dc.identifier.pmid11703080-
dc.identifier.scopuseid_2-s2.0-0035169502en_HK
dc.identifier.hkuros65863en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035169502&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume124en_HK
dc.identifier.issue2en_HK
dc.identifier.spage144en_HK
dc.identifier.epage151en_HK
dc.identifier.isiWOS:000172078100003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYeung, CM=7201354151en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK
dc.identifier.issnl0016-6480-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats