File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: In vitro and in vivo effects of adiponectin on bone

TitleIn vitro and in vivo effects of adiponectin on bone
Authors
Issue Date2009
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2009, v. 150 n. 8, p. 3603-3610 How to Cite?
AbstractFat mass impacts on both bone turnover and bone density and is a critical risk factor for osteoporotic fractures. Adipocyte-derived hormones may contribute to this relationship, and adiponectin is a principal circulating adipokine. However, its effects on bone remain unclear. We have, therefore, investigated the direct effects of adiponectin on primary cultures of osteoblastic and osteoclastic cells in vitro and determined its integrated effects in vivo by characterizing the bone phenotype of adiponectin-deficient mice. Adiponectin was dose-dependently mitogenic to primary rat and human osteoblasts (∼50% increase at 10 μg/ml) and markedly inhibited osteoclastogenesis at concentrations of 1 μg/ml or greater. It had no effect on osteoclastogenesis in RAW-264.7 cells or on bone resorption in isolated mature osteoclasts. In adiponectin knockout (AdKO) male C57BL/6J mice, trabecular bone volume and trabecular number (assessed by microcomputed tomography) were increased at 14 wk of age by 30% (P=0.02) and 38% (P=0.0009), respectively. Similar, nonsignificant trends were observed at 8 and 22 wk of age. Biomechanical testing showed lower bone fragility and reduced cortical hardness at 14 wk. We conclude that adiponectin stimulates osteoblast growth but inhibits osteoclastogenesis, probably via an effect on stromal cells. However, the AdKO mouse has increased bone mass, suggesting that adiponectin also has indirect effects on bone, possibly through modulating growth factor action or insulin sensitivity. Because adiponectin does influence bone mass in vivo, it is likely to be a contributor to the fat-bone relationship. Copyright © 2009 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/85465
ISSN
2023 Impact Factor: 3.8
2023 SCImago Journal Rankings: 1.285
ISI Accession Number ID
Funding AgencyGrant Number
Health Research Council of New Zealand
Funding Information:

This work was supported by the Health Research Council of New Zealand.

References

 

DC FieldValueLanguage
dc.contributor.authorWilliams, GAen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorCallon, KEen_HK
dc.contributor.authorWatson, Men_HK
dc.contributor.authorLin, JMen_HK
dc.contributor.authorLam, JBBen_HK
dc.contributor.authorCosta, JLen_HK
dc.contributor.authorOrpe, Aen_HK
dc.contributor.authorBroom, Nen_HK
dc.contributor.authorNaot, Den_HK
dc.contributor.authorReid, IRen_HK
dc.contributor.authorCornish, Jen_HK
dc.date.accessioned2010-09-06T09:05:14Z-
dc.date.available2010-09-06T09:05:14Z-
dc.date.issued2009en_HK
dc.identifier.citationEndocrinology, 2009, v. 150 n. 8, p. 3603-3610en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/85465-
dc.description.abstractFat mass impacts on both bone turnover and bone density and is a critical risk factor for osteoporotic fractures. Adipocyte-derived hormones may contribute to this relationship, and adiponectin is a principal circulating adipokine. However, its effects on bone remain unclear. We have, therefore, investigated the direct effects of adiponectin on primary cultures of osteoblastic and osteoclastic cells in vitro and determined its integrated effects in vivo by characterizing the bone phenotype of adiponectin-deficient mice. Adiponectin was dose-dependently mitogenic to primary rat and human osteoblasts (∼50% increase at 10 μg/ml) and markedly inhibited osteoclastogenesis at concentrations of 1 μg/ml or greater. It had no effect on osteoclastogenesis in RAW-264.7 cells or on bone resorption in isolated mature osteoclasts. In adiponectin knockout (AdKO) male C57BL/6J mice, trabecular bone volume and trabecular number (assessed by microcomputed tomography) were increased at 14 wk of age by 30% (P=0.02) and 38% (P=0.0009), respectively. Similar, nonsignificant trends were observed at 8 and 22 wk of age. Biomechanical testing showed lower bone fragility and reduced cortical hardness at 14 wk. We conclude that adiponectin stimulates osteoblast growth but inhibits osteoclastogenesis, probably via an effect on stromal cells. However, the AdKO mouse has increased bone mass, suggesting that adiponectin also has indirect effects on bone, possibly through modulating growth factor action or insulin sensitivity. Because adiponectin does influence bone mass in vivo, it is likely to be a contributor to the fat-bone relationship. Copyright © 2009 by The Endocrine Society.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsEndocrinology. Copyright © The Endocrine Society.en_HK
dc.titleIn vitro and in vivo effects of adiponectin on boneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=&spage=&epage=&date=2006&atitle=In+Vitro+and+In+Vivo+Effects+of+Adiponectin+on+Bone.en_HK
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.authorityWang, Y=rp00239en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2008-1639en_HK
dc.identifier.pmid19406946-
dc.identifier.scopuseid_2-s2.0-67651148206en_HK
dc.identifier.hkuros140351en_HK
dc.identifier.hkuros156260-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67651148206&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume150en_HK
dc.identifier.issue8en_HK
dc.identifier.spage3603en_HK
dc.identifier.epage3610en_HK
dc.identifier.isiWOS:000268158400022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f10001162823-
dc.identifier.scopusauthoridWilliams, GA=17136677600en_HK
dc.identifier.scopusauthoridWang, Y=34973733700en_HK
dc.identifier.scopusauthoridCallon, KE=35563288800en_HK
dc.identifier.scopusauthoridWatson, M=7403102175en_HK
dc.identifier.scopusauthoridLin, JM=25630762700en_HK
dc.identifier.scopusauthoridLam, JBB=24448474900en_HK
dc.identifier.scopusauthoridCosta, JL=36881215600en_HK
dc.identifier.scopusauthoridOrpe, A=37038263300en_HK
dc.identifier.scopusauthoridBroom, N=7004358087en_HK
dc.identifier.scopusauthoridNaot, D=7004280831en_HK
dc.identifier.scopusauthoridReid, IR=24475157600en_HK
dc.identifier.scopusauthoridCornish, J=7102230484en_HK
dc.identifier.issnl0013-7227-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats