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- Publisher Website: 10.1016/j.yjmcc.2006.10.002
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- PMID: 17112538
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Article: Evidence for cystic fibrosis transmembrane conductance regulator chloride current in swine ventricular myocytes
Title | Evidence for cystic fibrosis transmembrane conductance regulator chloride current in swine ventricular myocytes |
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Authors | |
Keywords | Cardiac myocytes CFTR Chloride channels Electrophysiology Heart Swine |
Issue Date | 2007 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjmcc |
Citation | Journal Of Molecular And Cellular Cardiology, 2007, v. 42 n. 1, p. 98-105 How to Cite? |
Abstract | The present study investigated whether cAMP-dependent cystic fibrosis transmembrane conductance regulator (CFTR) Cl - channel current (i.e., I Cl.CFTR or I Cl.cAMP) would be expressed in pig cardiac myocytes using whole-cell patch technique and reverse transcription polymerase chain reaction (RT-PCR). It was found that the β-adrenoceptor agonist isoproterenol activated a time-independent current in myocytes from the ventricle, but not the atrium of pig heart. Histamine and forskolin (an adenylate cyclase activator) induced a similar current in pig ventricular cells. The current induced by isoproterenol was blocked by the PKA inhibitor H-7, reduced by the replacement of external Cl - ion, and inhibited by the application of 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), but not 4′-diisothiocynatostilbene-2,2′-disulfonic acid (DIDS), typical of I Cl.CFTR. I Cl.CFTR showed a small difference in regional myocytes across the left ventricular wall from epicardium to endocardium. Isoproterenol-induced current was 3.1 ± 0.2 (n = 33), 2.8 ± 0.2 (n = 25) and 2.3 ± 0.2 pA/pF (n = 31) respectively in subepicardial, midmyocardial, and subendocardial myocytes (P < 0.05, subepicardium vs. subendocardium). RT-PCR and Western blotting analysis revealed that significant differences in CFTR channel mRNA and protein levels were present in atrial and ventricular cells, but not in regional ventricular cells across the ventricular wall from subepicardium to subendocardium. These results indicate that the functional CFTR channel (i.e., I Cl.CFTR) is present in ventricular myocytes, but not in atrial cells of pig heart. © 2006 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/85502 |
ISSN | 2023 Impact Factor: 4.9 2023 SCImago Journal Rankings: 1.639 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gao, Z | en_HK |
dc.contributor.author | Sun, HY | en_HK |
dc.contributor.author | Lau, CP | en_HK |
dc.contributor.author | ChinWan Fung, P | en_HK |
dc.contributor.author | Li, GR | en_HK |
dc.date.accessioned | 2010-09-06T09:05:50Z | - |
dc.date.available | 2010-09-06T09:05:50Z | - |
dc.date.issued | 2007 | en_HK |
dc.identifier.citation | Journal Of Molecular And Cellular Cardiology, 2007, v. 42 n. 1, p. 98-105 | en_HK |
dc.identifier.issn | 0022-2828 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/85502 | - |
dc.description.abstract | The present study investigated whether cAMP-dependent cystic fibrosis transmembrane conductance regulator (CFTR) Cl - channel current (i.e., I Cl.CFTR or I Cl.cAMP) would be expressed in pig cardiac myocytes using whole-cell patch technique and reverse transcription polymerase chain reaction (RT-PCR). It was found that the β-adrenoceptor agonist isoproterenol activated a time-independent current in myocytes from the ventricle, but not the atrium of pig heart. Histamine and forskolin (an adenylate cyclase activator) induced a similar current in pig ventricular cells. The current induced by isoproterenol was blocked by the PKA inhibitor H-7, reduced by the replacement of external Cl - ion, and inhibited by the application of 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), but not 4′-diisothiocynatostilbene-2,2′-disulfonic acid (DIDS), typical of I Cl.CFTR. I Cl.CFTR showed a small difference in regional myocytes across the left ventricular wall from epicardium to endocardium. Isoproterenol-induced current was 3.1 ± 0.2 (n = 33), 2.8 ± 0.2 (n = 25) and 2.3 ± 0.2 pA/pF (n = 31) respectively in subepicardial, midmyocardial, and subendocardial myocytes (P < 0.05, subepicardium vs. subendocardium). RT-PCR and Western blotting analysis revealed that significant differences in CFTR channel mRNA and protein levels were present in atrial and ventricular cells, but not in regional ventricular cells across the ventricular wall from subepicardium to subendocardium. These results indicate that the functional CFTR channel (i.e., I Cl.CFTR) is present in ventricular myocytes, but not in atrial cells of pig heart. © 2006 Elsevier Inc. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjmcc | en_HK |
dc.relation.ispartof | Journal of Molecular and Cellular Cardiology | en_HK |
dc.subject | Cardiac myocytes | - |
dc.subject | CFTR | - |
dc.subject | Chloride channels | - |
dc.subject | Electrophysiology | - |
dc.subject | Heart | - |
dc.subject | Swine | - |
dc.subject.mesh | 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Base Sequence | en_HK |
dc.subject.mesh | Cystic Fibrosis Transmembrane Conductance Regulator - genetics - metabolism | en_HK |
dc.subject.mesh | DNA Primers - genetics | en_HK |
dc.subject.mesh | Endocardium - cytology - metabolism | en_HK |
dc.subject.mesh | Heart Atria - cytology - metabolism | en_HK |
dc.subject.mesh | Heart Ventricles - cytology - metabolism | en_HK |
dc.subject.mesh | Isoproterenol - pharmacology | en_HK |
dc.subject.mesh | Membrane Potentials - drug effects | en_HK |
dc.subject.mesh | Myocardium - cytology - metabolism | en_HK |
dc.subject.mesh | Myocytes, Cardiac - drug effects - metabolism | en_HK |
dc.subject.mesh | Pericardium - cytology - metabolism | en_HK |
dc.subject.mesh | RNA, Messenger - genetics - metabolism | en_HK |
dc.subject.mesh | Swine | en_HK |
dc.subject.mesh | Tissue Distribution | en_HK |
dc.title | Evidence for cystic fibrosis transmembrane conductance regulator chloride current in swine ventricular myocytes | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-2828&volume=42&issue=1&spage=98&epage=105&date=2007&atitle=Evidence+for+cystic+fibrosis+transmembrane+conductance+regulator+chloride+current+in+swine+ventricular+myocytes | en_HK |
dc.identifier.email | Li, GR:grli@hkucc.hku.hk | en_HK |
dc.identifier.authority | Li, GR=rp00476 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.yjmcc.2006.10.002 | en_HK |
dc.identifier.pmid | 17112538 | - |
dc.identifier.scopus | eid_2-s2.0-33845743367 | en_HK |
dc.identifier.hkuros | 125283 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33845743367&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 42 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 98 | en_HK |
dc.identifier.epage | 105 | en_HK |
dc.identifier.isi | WOS:000243617100011 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Gao, Z=16549711200 | en_HK |
dc.identifier.scopusauthorid | Sun, HY=35723049200 | en_HK |
dc.identifier.scopusauthorid | Lau, CP=7401968501 | en_HK |
dc.identifier.scopusauthorid | ChinWan Fung, P=6504642578 | en_HK |
dc.identifier.scopusauthorid | Li, GR=7408462932 | en_HK |
dc.identifier.issnl | 0022-2828 | - |