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Conference Paper: Over-express endothelin-1 in astrocyte lead to increased brain damage and altered cytokine expression after cerebrl ischemia
Title | Over-express endothelin-1 in astrocyte lead to increased brain damage and altered cytokine expression after cerebrl ischemia |
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Authors | |
Keywords | Astrocytes Cytokines Endothelin Ischemia Transgenic mouse |
Issue Date | 2003 |
Publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1471-4159 |
Citation | Journal of Neurochemistry, 2003, v. 87 suppl. s1, p. 150, abstract no. P14-27 How to Cite? |
Abstract | Previously, we reported that the expression of endothelin (ET-1) is induced in
astrocytes and endothelial cells after cerebral hypoxia/ischemia. Recently, we
also showed that transgenic mice over-expressing ET-1 in astrocytes
displayed more severe neurologic deficits and increased brain infract
following transient middle cerebral artery occlusion (MCAO for 2 h
followed by 22 h reperfusion) compared with those of non-transgenic mice.
However, the underling mechanism for further brain damage in these
transgenic mice with astrocytic ET-1 over-expression is not yet clear. ET-1
has been shown to stimulate leukotriene (LTB4) and cytokine expression
such as interleukin 6 (IL-6) after MCAO, which may contribute to brain
inflammation and damage. Here, we investigated cytokine expression in
brain of astrocytic ET-1 transgenic mice to determine whether the altered
cytokine expressions may have lead to further brain damage in these
transgenic mice. Group of transgenic and non-transgenic mice were exposed
to either sham or transient MCAO. Cytokine mRNAs from these mouse
brains were detected by using the RiboQuant multi-Probe Ribonuclease
Protection Assay system. The brain of astrocytic ET-1 transgenic mice
treated with the transient MCAO showed increased level of macrophage
migration inhibitory factor (MIF), whereas IL-6 level did not show any
changes. The present data suggest that altered expression of MIF in the brain
of astrocytic ET-1 transgenic mice may have contributed to more severe
neurologic deficits and increased brain damage after MCAO. |
Description | Poster Session P14: Neurotrauma and Injury, Pain, Regeneration and Repair |
Persistent Identifier | http://hdl.handle.net/10722/88037 |
ISSN | 2023 Impact Factor: 4.2 2023 SCImago Journal Rankings: 1.476 |
DC Field | Value | Language |
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dc.contributor.author | Wu, WH | - |
dc.contributor.author | Lo, ACY | - |
dc.contributor.author | Chung, SK | - |
dc.date.accessioned | 2010-09-06T09:37:49Z | - |
dc.date.available | 2010-09-06T09:37:49Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Journal of Neurochemistry, 2003, v. 87 suppl. s1, p. 150, abstract no. P14-27 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.uri | http://hdl.handle.net/10722/88037 | - |
dc.description | Poster Session P14: Neurotrauma and Injury, Pain, Regeneration and Repair | - |
dc.description.abstract | Previously, we reported that the expression of endothelin (ET-1) is induced in astrocytes and endothelial cells after cerebral hypoxia/ischemia. Recently, we also showed that transgenic mice over-expressing ET-1 in astrocytes displayed more severe neurologic deficits and increased brain infract following transient middle cerebral artery occlusion (MCAO for 2 h followed by 22 h reperfusion) compared with those of non-transgenic mice. However, the underling mechanism for further brain damage in these transgenic mice with astrocytic ET-1 over-expression is not yet clear. ET-1 has been shown to stimulate leukotriene (LTB4) and cytokine expression such as interleukin 6 (IL-6) after MCAO, which may contribute to brain inflammation and damage. Here, we investigated cytokine expression in brain of astrocytic ET-1 transgenic mice to determine whether the altered cytokine expressions may have lead to further brain damage in these transgenic mice. Group of transgenic and non-transgenic mice were exposed to either sham or transient MCAO. Cytokine mRNAs from these mouse brains were detected by using the RiboQuant multi-Probe Ribonuclease Protection Assay system. The brain of astrocytic ET-1 transgenic mice treated with the transient MCAO showed increased level of macrophage migration inhibitory factor (MIF), whereas IL-6 level did not show any changes. The present data suggest that altered expression of MIF in the brain of astrocytic ET-1 transgenic mice may have contributed to more severe neurologic deficits and increased brain damage after MCAO. | - |
dc.language | eng | - |
dc.publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1471-4159 | - |
dc.relation.ispartof | Journal of Neurochemistry | - |
dc.subject | Astrocytes | - |
dc.subject | Cytokines | - |
dc.subject | Endothelin | - |
dc.subject | Ischemia | - |
dc.subject | Transgenic mouse | - |
dc.title | Over-express endothelin-1 in astrocyte lead to increased brain damage and altered cytokine expression after cerebrl ischemia | - |
dc.type | Conference_Paper | - |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3042&volume=87&spage=150 Suppl. 1&epage=&date=2003&atitle=Over-express+endothelin-1+in+astrocyte+lead+to+increased+brain+damage+and+altered+cytokine+expression+after+cerebrl+ischemia | en_HK |
dc.identifier.email | Lo, ACY: amylo@.hku.hk | - |
dc.identifier.email | Chung, SK: skchung@hkucc.hku.hk | - |
dc.identifier.authority | Lo, ACY=rp00425 | - |
dc.identifier.authority | Chung, SK=rp00381 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1046/j.1474-1644.2003.2175_14.x | - |
dc.identifier.hkuros | 113543 | - |
dc.identifier.volume | 87 | - |
dc.identifier.issue | suppl. s1 | - |
dc.identifier.spage | 150, abstract no. P14-27 | - |
dc.identifier.epage | 150, abstract no. P14-27 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 0022-3042 | - |