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- Publisher Website: 10.1006/bbrc.2001.5349
- Scopus: eid_2-s2.0-0034816421
- PMID: 11485313
- WOS: WOS:000170519100015
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Article: Transcriptional activity of the promoter region of rat frizzled-related protein gene
Title | Transcriptional activity of the promoter region of rat frizzled-related protein gene |
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Authors | |
Keywords | Frizzled related protein Mutant p53 Promoter Transcriptional regulation |
Issue Date | 2001 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description |
Citation | Biochemical And Biophysical Research Communications, 2001, v. 286 n. 1, p. 94-100 How to Cite? |
Abstract | Frizzled-related protein (Frp) is a new family of secreted proteins involved in tumorigenesis and Wnt-signaling pathway. Previous study has shown that rat Frp (rFrp) gene was found to be differentially expressed in Rat 6 fibroblast cell line overexpressing p53val135 (R6#13-8). The rFrp gene was otherwise silent in normal parental Rat 6 cells. To elucidate the molecular basis of the transcriptional activation of rFrp, we have isolated and analyzed a 2-kilobase pair promoter region of the rFrp gene. Mapping of transcription initiation sites of rFrp showed the existence of multiple initiation sites. Transfection studies of serial deletion constructs in both Rat 6 and CHOK1 cell lines revealed that the region from -202 to -144 contains cis-acting elements essential for the efficient transcription of rFrp. This work provides a transcriptional regulation basis for Frp and gives insight into its implication in tumorigenesis. © 2001 Academic Press. |
Persistent Identifier | http://hdl.handle.net/10722/88520 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Ping Yam, JW | en_HK |
dc.contributor.author | Chan, KW | en_HK |
dc.contributor.author | Wai Wong, VK | en_HK |
dc.contributor.author | Wendy Hsiao, WL | en_HK |
dc.date.accessioned | 2010-09-06T09:44:26Z | - |
dc.date.available | 2010-09-06T09:44:26Z | - |
dc.date.issued | 2001 | en_HK |
dc.identifier.citation | Biochemical And Biophysical Research Communications, 2001, v. 286 n. 1, p. 94-100 | en_HK |
dc.identifier.issn | 0006-291X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/88520 | - |
dc.description.abstract | Frizzled-related protein (Frp) is a new family of secreted proteins involved in tumorigenesis and Wnt-signaling pathway. Previous study has shown that rat Frp (rFrp) gene was found to be differentially expressed in Rat 6 fibroblast cell line overexpressing p53val135 (R6#13-8). The rFrp gene was otherwise silent in normal parental Rat 6 cells. To elucidate the molecular basis of the transcriptional activation of rFrp, we have isolated and analyzed a 2-kilobase pair promoter region of the rFrp gene. Mapping of transcription initiation sites of rFrp showed the existence of multiple initiation sites. Transfection studies of serial deletion constructs in both Rat 6 and CHOK1 cell lines revealed that the region from -202 to -144 contains cis-acting elements essential for the efficient transcription of rFrp. This work provides a transcriptional regulation basis for Frp and gives insight into its implication in tumorigenesis. © 2001 Academic Press. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | en_HK |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_HK |
dc.subject | Frizzled related protein | - |
dc.subject | Mutant p53 | - |
dc.subject | Promoter | - |
dc.subject | Transcriptional regulation | - |
dc.subject.mesh | Amino Acid Sequence | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Base Sequence | en_HK |
dc.subject.mesh | CHO Cells | en_HK |
dc.subject.mesh | Cell Line | en_HK |
dc.subject.mesh | Cricetinae | en_HK |
dc.subject.mesh | DNA | en_HK |
dc.subject.mesh | Molecular Sequence Data | en_HK |
dc.subject.mesh | Promoter Regions, Genetic | en_HK |
dc.subject.mesh | Proteins - genetics | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Transcription, Genetic | en_HK |
dc.title | Transcriptional activity of the promoter region of rat frizzled-related protein gene | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=286&spage=94&epage=100&date=2001&atitle=Transcriptional+activity+of+the+promoter+region+of+rat+Frizzled-related+protein+gene | en_HK |
dc.identifier.email | Ping Yam, JW:judyyam@pathology.hku.hk | en_HK |
dc.identifier.authority | Ping Yam, JW=rp00468 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1006/bbrc.2001.5349 | en_HK |
dc.identifier.pmid | 11485313 | - |
dc.identifier.scopus | eid_2-s2.0-0034816421 | en_HK |
dc.identifier.hkuros | 63383 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034816421&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 286 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 94 | en_HK |
dc.identifier.epage | 100 | en_HK |
dc.identifier.isi | WOS:000170519100015 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.issnl | 0006-291X | - |