File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1177/0961203306075793
- Scopus: eid_2-s2.0-33847374613
- PMID: 17402368
- WOS: WOS:000244665800007
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Abnormality of bone marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus
Title | Abnormality of bone marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus |
---|---|
Authors | |
Keywords | Biological character Cytokine Hematopoietic support Mesenchymal stem cells Systemic lupus erythematosus |
Issue Date | 2007 |
Publisher | Sage Publications Ltd. The Journal's web site is located at http://lup.sagepub.com |
Citation | Lupus, 2007, v. 16 n. 2, p. 121-128 How to Cite? |
Abstract | Bone marrow-derived mesenchymal stem cells (MSCs) are key components of the hematopoietic microenvironment and provide support to hematopoiesis and modulate immune system. Several studies suggest that SLE may be seen as stem cell disorders. However, it is unclear that whether MSCs from SLE patients are defective. So in this research, we studied the biological character of bone marrow derived MSCs in patients with SLE, focused on their phenotype (morphology and immunophenotype), karyotype, cytokines expression and hematopoietic support of MSCs. Our results showed that MSCs from SLE patients and normal controls can be successfully culture-expanded, but the MSCs from SLE grew more slowly than those of normal controls (P < 0.05). Cells from both groups were positive for CD29, CD44 and CD105, and negative for CD14, CD34, CD45 and HLA-DR. MSCs from SLE have a normal karyotype. Both groups express IL-6, IL7, IL-11, macrophage colony stimulating factor (M-CSF) and stem cell factor (SCF) at mRNA level. While IL-6 and IL-7 were down-regulated in MSCs from SLE patient (P < 0.05) at mRNA level. The MSCs from SLE patients and normal controls were infused into ICR (Tac : Icr : Ha strain) mice after high-dose chemotherapy, with no adverse events in either group. Recovery of white blood cells, hemoglobin and platelet was more rapid (P < 0.05) compared with the group without MSCs infusion. We conclude that MSCs in patient with SLE have abnormalities compared with those in normal control. MSCs in patient with SLE may play an important role in the SLE pathogenesis. © 2007 SAGE Publications. |
Persistent Identifier | http://hdl.handle.net/10722/88557 |
ISSN | 2023 Impact Factor: 1.9 2023 SCImago Journal Rankings: 0.812 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sun, LY | en_HK |
dc.contributor.author | Zhang, HY | en_HK |
dc.contributor.author | Feng, XB | en_HK |
dc.contributor.author | Hou, YY | en_HK |
dc.contributor.author | Lu, LW | en_HK |
dc.contributor.author | Fan, LM | en_HK |
dc.date.accessioned | 2010-09-06T09:44:55Z | - |
dc.date.available | 2010-09-06T09:44:55Z | - |
dc.date.issued | 2007 | en_HK |
dc.identifier.citation | Lupus, 2007, v. 16 n. 2, p. 121-128 | en_HK |
dc.identifier.issn | 0961-2033 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/88557 | - |
dc.description.abstract | Bone marrow-derived mesenchymal stem cells (MSCs) are key components of the hematopoietic microenvironment and provide support to hematopoiesis and modulate immune system. Several studies suggest that SLE may be seen as stem cell disorders. However, it is unclear that whether MSCs from SLE patients are defective. So in this research, we studied the biological character of bone marrow derived MSCs in patients with SLE, focused on their phenotype (morphology and immunophenotype), karyotype, cytokines expression and hematopoietic support of MSCs. Our results showed that MSCs from SLE patients and normal controls can be successfully culture-expanded, but the MSCs from SLE grew more slowly than those of normal controls (P < 0.05). Cells from both groups were positive for CD29, CD44 and CD105, and negative for CD14, CD34, CD45 and HLA-DR. MSCs from SLE have a normal karyotype. Both groups express IL-6, IL7, IL-11, macrophage colony stimulating factor (M-CSF) and stem cell factor (SCF) at mRNA level. While IL-6 and IL-7 were down-regulated in MSCs from SLE patient (P < 0.05) at mRNA level. The MSCs from SLE patients and normal controls were infused into ICR (Tac : Icr : Ha strain) mice after high-dose chemotherapy, with no adverse events in either group. Recovery of white blood cells, hemoglobin and platelet was more rapid (P < 0.05) compared with the group without MSCs infusion. We conclude that MSCs in patient with SLE have abnormalities compared with those in normal control. MSCs in patient with SLE may play an important role in the SLE pathogenesis. © 2007 SAGE Publications. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Sage Publications Ltd. The Journal's web site is located at http://lup.sagepub.com | en_HK |
dc.relation.ispartof | Lupus | en_HK |
dc.rights | Lupus. Copyright © Sage Publications Ltd. | en_HK |
dc.subject | Biological character | - |
dc.subject | Cytokine | - |
dc.subject | Hematopoietic support | - |
dc.subject | Mesenchymal stem cells | - |
dc.subject | Systemic lupus erythematosus | - |
dc.subject.mesh | Adolescent | en_HK |
dc.subject.mesh | Adult | en_HK |
dc.subject.mesh | Bone Marrow Cells | en_HK |
dc.subject.mesh | Cell Proliferation | en_HK |
dc.subject.mesh | Female | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Karyotyping | en_HK |
dc.subject.mesh | Lupus Erythematosus, Systemic - immunology - pathology | en_HK |
dc.subject.mesh | Mesenchymal Stem Cells - immunology - pathology | en_HK |
dc.subject.mesh | Middle Aged | en_HK |
dc.title | Abnormality of bone marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0961-2033&volume=16&issue=2&spage=121&epage=8&date=2007&atitle=Abnormality+of+bone+marrow-derived+mesenchymal+stem+cells+in+patients+with+systemic+lupus+erythematosus | en_HK |
dc.identifier.email | Lu, LW:liweilu@hkucc.hku.hk | en_HK |
dc.identifier.authority | Lu, LW=rp00477 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1177/0961203306075793 | en_HK |
dc.identifier.pmid | 17402368 | - |
dc.identifier.scopus | eid_2-s2.0-33847374613 | en_HK |
dc.identifier.hkuros | 132279 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33847374613&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 16 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 121 | en_HK |
dc.identifier.epage | 128 | en_HK |
dc.identifier.isi | WOS:000244665800007 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.issnl | 0961-2033 | - |