四種中藥複方對急性心理應激大鼠IL-1β、IL-2的影響

Abstract

目的通過觀察理氣、化痰、補氣、溫陽方藥對急性心理應激大鼠白細胞介素1β(IL1β)、白細胞介素2(IL2)的影響,研究中藥復方對急性應激免疫內分泌的調節作用。方法將大鼠分為7組:正常組、模型Ⅰ組、模型Ⅱ組、四逆散組、半夏厚樸湯組、甘麥大棗湯組、桂枝甘草龍骨牡蠣湯組;觀察各組大鼠的胸腺指數、脾指數及腎上腺指數,采用放免法測定各組大鼠血清IL1β、IL2的含量。結果急性心理應激后大鼠血清IL1β升高,IL2降低,胸腺指數、脾指數降低(P<0.01),腎上腺指數無變化(P>0.05);四逆散升高胸腺指數、脾指數(P<0.01、P<0.05),降低IL1β(P<0.01);半夏厚樸湯降低IL1β(P<0.01、P<0.05),升高IL2(P<0.05);甘麥大棗湯升高脾指數,降低IL1β(P<0.05);桂枝甘草龍骨牡蠣湯升高胸腺指數(P<0.01),降低IL1β的含量(P<0.01),升高IL2的含量(P<0.01)。結論四種中藥復方通過作用于應激大鼠細胞因子及下丘腦-垂體-腎上腺軸的不同環節,調節急性心理應激后大鼠的免疫內分泌。

Objective In order to study the regulating effects of traditional Chinese herbal compound formulae on the immuno-endocrine network of psychological stress rats, four kinds of traditional Chinese herbal compound formulae, respectively acting on regulating qi, dispersing phlegm, tonifying qi and warming Yang, were used to treat the acute psychological stress rats, and the serum contents of IL-1βand IL-2 were assayed. Methods Rats were divided into 7 groups: normal group, model 1 and 2 groups, Sini Powder group, Banxiahoupu Decoction group, Ganmaidazao Decoction group, and Guizhigancaolonggumuli Decoction group. The indexes of thymus gland, spleen and adrenal gland were observed; the serum contents of IL-1βand IL-2 of all the rats were detected by the means of radioimmunoassay. Results In acute psychological stress rats,the serum content of IL-1β was higher, but the level of IL-2, the indexes of thymus gland and the spleen were lower (P<0.01), while the index of adrenal gland had not any obvious change (P>0.05); Sini Powder increased the indexes of thymus gland and the spleen (P<0.01, P<0.05), and decreased content of serum IL-1β(P<0.01); Banxiahoupu Decoction decreased serum content of IL-1β(P<0.01, P<0.05) and increased the content of IL-2 (P<0.05); Ganmaidazao Decoction increased the index of the spleen, and decreased the content of IL-1β(P<0.05); Guizhigancaolonggumuli Decoction increased the index of the thymus gland and the content of IL-2 (P<0.01),and decreased the content of IL-1β(P<0.01). Conclusion The four kinds of traditional Chinese herbal compound formulae can regulate immuno-endocrinel network of the acute psychological stress rats through the action on the cytokine and the different taches of the hypothalamus-pituitary-adrenal gland axise.