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- Publisher Website: 10.1023/A:1008008400653
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- PMID: 10403698
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Article: A defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in potexviruses
Title | A defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in potexviruses |
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Authors | |
Keywords | Species Index: Bamboo Mosaic Virus Nicotiana Benthamiana Potexvirus Rna Viruses |
Issue Date | 1999 |
Publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-8569 |
Citation | Virus Genes, 1999, v. 18 n. 2, p. 121-128 How to Cite? |
Abstract | A naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5' terminus and 148 nts of the 3' terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5' and 3' junction borders of reported potexvirus D RNAs reveal a 65.2-84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of potexvirus D RNAs. |
Persistent Identifier | http://hdl.handle.net/10722/90840 |
ISSN | 2023 Impact Factor: 1.9 2023 SCImago Journal Rankings: 0.443 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yeh, T-Y | en_HK |
dc.contributor.author | Lin, B-Y | en_HK |
dc.contributor.author | Chang, Y-C | en_HK |
dc.contributor.author | Hsu, Y-H | en_HK |
dc.contributor.author | Lin, N-S | en_HK |
dc.date.accessioned | 2010-09-17T10:09:10Z | - |
dc.date.available | 2010-09-17T10:09:10Z | - |
dc.date.issued | 1999 | en_HK |
dc.identifier.citation | Virus Genes, 1999, v. 18 n. 2, p. 121-128 | en_HK |
dc.identifier.issn | 0920-8569 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/90840 | - |
dc.description.abstract | A naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5' terminus and 148 nts of the 3' terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5' and 3' junction borders of reported potexvirus D RNAs reveal a 65.2-84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of potexvirus D RNAs. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Springer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-8569 | en_HK |
dc.relation.ispartof | Virus Genes | en_HK |
dc.subject | Species Index: Bamboo Mosaic Virus | en_HK |
dc.subject | Nicotiana Benthamiana | en_HK |
dc.subject | Potexvirus | en_HK |
dc.subject | Rna Viruses | en_HK |
dc.title | A defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in potexviruses | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lin, B:blin@hku.hk | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1023/A:1008008400653 | en_HK |
dc.identifier.pmid | 10403698 | - |
dc.identifier.scopus | eid_2-s2.0-0033048669 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0033048669&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 18 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 121 | en_HK |
dc.identifier.epage | 128 | en_HK |
dc.identifier.isi | WOS:000080737100004 | - |
dc.identifier.issnl | 0920-8569 | - |