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- Publisher Website: 10.1016/S1005-9040(06)60076-7
- Scopus: eid_2-s2.0-33646381349
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Article: Construction of high expression plasmid of human augmenter of liver regeneration(hALR), expression and purification of hALR
Title | Construction of high expression plasmid of human augmenter of liver regeneration(hALR), expression and purification of hALR |
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Authors | |
Keywords | Augmenter Of Liver Regeneration Fad-Dependent Sulfhydryl Oxidase Prokayotic Expression Plasmid |
Issue Date | 2006 |
Publisher | Jilin Daxue. The Journal's web site is located at http://www.cjcu.jlu.edu.cn/ |
Citation | Chemical Research in Chinese Universities, 2006, v. 22 n. 2, p. 201-204 How to Cite? |
Abstract | Experimental evidence has been presented to suggest that the human augmenter of liver regeneration (hALR) serves as a hepatotrophic growth factor during liver regeneration and as a generalized growth factor during pancreas transplant/regeneration. A prokaryotic expression plasmid, pRSET/6his-c-myc-hALR was constructed, by cloning synthesized hALR cDNA into pRSET/6his-c-myc that was improved on the basis of pRSET B by the group. As a result, the protein was highly expressed in E. coli BL21. The recombinant hALR was over 60% of the total protein in E. coli. Its validity was confirmed by means of Western Blotting. The protein was purified by Ni-NTA affinity chromatography and this FAD-dependent sulfhydryl oxidase activity was measured. |
Persistent Identifier | http://hdl.handle.net/10722/91241 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.620 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Sun, T-X | en_HK |
dc.contributor.author | Wu, Y-G | en_HK |
dc.contributor.author | Yu, X-H | en_HK |
dc.contributor.author | Jiang, C-L | en_HK |
dc.contributor.author | Jin, Y-H | en_HK |
dc.contributor.author | Cheng, Y | en_HK |
dc.contributor.author | Kong, W | en_HK |
dc.date.accessioned | 2010-09-17T10:15:29Z | - |
dc.date.available | 2010-09-17T10:15:29Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | Chemical Research in Chinese Universities, 2006, v. 22 n. 2, p. 201-204 | en_HK |
dc.identifier.issn | 1005-9040 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/91241 | - |
dc.description.abstract | Experimental evidence has been presented to suggest that the human augmenter of liver regeneration (hALR) serves as a hepatotrophic growth factor during liver regeneration and as a generalized growth factor during pancreas transplant/regeneration. A prokaryotic expression plasmid, pRSET/6his-c-myc-hALR was constructed, by cloning synthesized hALR cDNA into pRSET/6his-c-myc that was improved on the basis of pRSET B by the group. As a result, the protein was highly expressed in E. coli BL21. The recombinant hALR was over 60% of the total protein in E. coli. Its validity was confirmed by means of Western Blotting. The protein was purified by Ni-NTA affinity chromatography and this FAD-dependent sulfhydryl oxidase activity was measured. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Jilin Daxue. The Journal's web site is located at http://www.cjcu.jlu.edu.cn/ | en_HK |
dc.relation.ispartof | Chemical Research in Chinese Universities | en_HK |
dc.subject | Augmenter Of Liver Regeneration | en_HK |
dc.subject | Fad-Dependent Sulfhydryl Oxidase | en_HK |
dc.subject | Prokayotic Expression Plasmid | en_HK |
dc.title | Construction of high expression plasmid of human augmenter of liver regeneration(hALR), expression and purification of hALR | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Cheng, Y:yuecheng@hku.hk | en_HK |
dc.identifier.authority | Cheng, Y=rp1320 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/S1005-9040(06)60076-7 | en_HK |
dc.identifier.scopus | eid_2-s2.0-33646381349 | en_HK |
dc.identifier.volume | 22 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 201 | en_HK |
dc.identifier.epage | 204 | en_HK |
dc.identifier.isi | WOS:000236560900018 | - |
dc.identifier.issnl | 1005-9040 | - |