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Article: Transcriptional and functional profilling of human embryonic stem cell-derived cardiomyocytes
Title | Transcriptional and functional profilling of human embryonic stem cell-derived cardiomyocytes | ||||||||||
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Authors | |||||||||||
Issue Date | 2008 | ||||||||||
Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||||||
Citation | Plos One, 2008, v. 3 n. 10 How to Cite? | ||||||||||
Abstract | Human embryonic stemcells (hESCs) can serve as a potentially limitless source of cells that may enable regeneration of diseased tissue and organs. Here we investigate the use of human embryonic stemcell-derived cardiomyocytes (hESC-CMs) in promoting recovery from cardiac ischemia reperfusion injury in a mouse model. Using microarrays, we have described the hESC-CM transcriptome within the spectrum of changes that occur between undifferentiated hESCs and fetal heart cells. The hESC-CMs expressed cardiomyocyte genes at levels similar to those found in 20-week fetal heart cells, making this population a good source of potential replacement cells in vivo. Echocardiographic studies showed significant improvement in heart function by 8 weeks after transplantation. Finally, we demonstrate long-term engraftment of hESC-CMs by using molecular imaging to track cellular localization, survival, and proliferation in vivo. Taken together, global gene expression profiling of hESC differentiation enables a systems-based analysis of the biological processes, networks, and genes that drive hESC fate decisions, and studies such as this will serve as the foundation for future clinical applications of stem cell therapies. © 2008 Cao et al. | ||||||||||
Persistent Identifier | http://hdl.handle.net/10722/91518 | ||||||||||
ISSN | 2021 Impact Factor: 3.752 2020 SCImago Journal Rankings: 0.990 | ||||||||||
PubMed Central ID | |||||||||||
ISI Accession Number ID |
Funding Information: This work was supported by grants from the BWF CAMS, Stanford Cardiovascular Institute, NIH HL089027, and CIRM RS1-00322 (JCW). This work was also supported in part by NIH CA114747 (SSG), NIH HL076445 (RAW), and NIH HL72857 (RAL). | ||||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cao, F | en_HK |
dc.contributor.author | Wagner, RA | en_HK |
dc.contributor.author | Wilson, KD | en_HK |
dc.contributor.author | Xie, X | en_HK |
dc.contributor.author | Fu, JD | en_HK |
dc.contributor.author | Drukker, M | en_HK |
dc.contributor.author | Lee, A | en_HK |
dc.contributor.author | Li, RA | en_HK |
dc.contributor.author | Gambhir, SS | en_HK |
dc.contributor.author | Weissman, IL | en_HK |
dc.contributor.author | Robbins, RC | en_HK |
dc.contributor.author | Wu, JC | en_HK |
dc.date.accessioned | 2010-09-17T10:20:42Z | - |
dc.date.available | 2010-09-17T10:20:42Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Plos One, 2008, v. 3 n. 10 | en_HK |
dc.identifier.issn | 1932-6203 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/91518 | - |
dc.description.abstract | Human embryonic stemcells (hESCs) can serve as a potentially limitless source of cells that may enable regeneration of diseased tissue and organs. Here we investigate the use of human embryonic stemcell-derived cardiomyocytes (hESC-CMs) in promoting recovery from cardiac ischemia reperfusion injury in a mouse model. Using microarrays, we have described the hESC-CM transcriptome within the spectrum of changes that occur between undifferentiated hESCs and fetal heart cells. The hESC-CMs expressed cardiomyocyte genes at levels similar to those found in 20-week fetal heart cells, making this population a good source of potential replacement cells in vivo. Echocardiographic studies showed significant improvement in heart function by 8 weeks after transplantation. Finally, we demonstrate long-term engraftment of hESC-CMs by using molecular imaging to track cellular localization, survival, and proliferation in vivo. Taken together, global gene expression profiling of hESC differentiation enables a systems-based analysis of the biological processes, networks, and genes that drive hESC fate decisions, and studies such as this will serve as the foundation for future clinical applications of stem cell therapies. © 2008 Cao et al. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | en_HK |
dc.relation.ispartof | PLoS ONE | en_HK |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject.mesh | Embryonic Stem Cells - cytology - transplantation | - |
dc.subject.mesh | Gene Expression Profiling | - |
dc.subject.mesh | Myocardial Reperfusion Injury - therapy | - |
dc.subject.mesh | Myocytes, Cardiac - cytology - transplantation | - |
dc.subject.mesh | Stem Cell Transplantation - methods | - |
dc.title | Transcriptional and functional profilling of human embryonic stem cell-derived cardiomyocytes | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1932-6203&volume=3&issue=10&spage=e3474&epage=&date=2008&atitle=Transcriptional+and+Functional+Profiling+of+Human+Embryonic+Stem+Cell-Derived+Cardiomyocytes | - |
dc.identifier.email | Li, RA:ronaldli@hkucc.hku.hk | en_HK |
dc.identifier.authority | Li, RA=rp01352 | en_HK |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1371/journal.pone.0003474 | en_HK |
dc.identifier.pmid | 18941512 | - |
dc.identifier.pmcid | PMC2565131 | - |
dc.identifier.scopus | eid_2-s2.0-55649098531 | en_HK |
dc.identifier.hkuros | 182849 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-55649098531&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 3 | en_HK |
dc.identifier.issue | 10 | en_HK |
dc.identifier.spage | e3474 | - |
dc.identifier.epage | e3474 | - |
dc.identifier.isi | WOS:000265126100005 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Cao, F=35072497500 | en_HK |
dc.identifier.scopusauthorid | Wagner, RA=7404292272 | en_HK |
dc.identifier.scopusauthorid | Wilson, KD=24726465500 | en_HK |
dc.identifier.scopusauthorid | Xie, X=12786458300 | en_HK |
dc.identifier.scopusauthorid | Fu, JD=7401722481 | en_HK |
dc.identifier.scopusauthorid | Drukker, M=12787035400 | en_HK |
dc.identifier.scopusauthorid | Lee, A=35790108100 | en_HK |
dc.identifier.scopusauthorid | Li, RA=7404724466 | en_HK |
dc.identifier.scopusauthorid | Gambhir, SS=7101904349 | en_HK |
dc.identifier.scopusauthorid | Weissman, IL=36038861200 | en_HK |
dc.identifier.scopusauthorid | Robbins, RC=7202460248 | en_HK |
dc.identifier.scopusauthorid | Wu, JC=7409253259 | en_HK |
dc.identifier.issnl | 1932-6203 | - |