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- Publisher Website: 10.1016/j.colsurfb.2003.08.016
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Article: Estimating the size of laterally phase separated cholesterol domains in model membranes with Förster resonance energy transfer: A simulation study
Title | Estimating the size of laterally phase separated cholesterol domains in model membranes with Förster resonance energy transfer: A simulation study |
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Authors | |
Keywords | Bilayer Cholesterol Domains FRET Membranes |
Issue Date | 2004 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/colsurfb |
Citation | Colloids And Surfaces B: Biointerfaces, 2004, v. 33 n. 1, p. 57-65 How to Cite? |
Abstract | In this work, we use two vertically-coupled square two-dimensional lattices to simulate membrane bilayers containing a uniform size distribution of cholesterol immiscible domains of a predetermined size distribution. We substitute cholesterols and phospholipids with their fluorescent analogs and calculate the efficiency of energy transfer as a function of acceptor concentration for four membrane configurations. The simulated efficiency of energy transfer as a function of acceptor concentration data is then fit with an analytical FRET model to estimate the domain size, in the same manner in which experimental FRET data is analyzed. The fitted model parameters (domain size and donor partition coefficient) are compared to the simulation inputs to test the applicability of the FRET model to estimating the size of laterally phase separated cholesterol domains. We show that the FRET model yields good size estimates for domains that range between 1 and 25nm. We also find that the assumed fluorophore configuration in the FRET model leads to a constant under-prediction of these values. Finally, we demonstrate that when two parameters are open to the fit, the FRET model adequately predicts the donor partition coefficient in addition to the domain size. © 2003 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/92475 |
ISSN | 2023 Impact Factor: 5.4 2023 SCImago Journal Rankings: 0.910 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Troup, GM | en_HK |
dc.contributor.author | Tulenko, TN | en_HK |
dc.contributor.author | Lee, SP | en_HK |
dc.contributor.author | Wrenn, SP | en_HK |
dc.date.accessioned | 2010-09-17T10:47:23Z | - |
dc.date.available | 2010-09-17T10:47:23Z | - |
dc.date.issued | 2004 | en_HK |
dc.identifier.citation | Colloids And Surfaces B: Biointerfaces, 2004, v. 33 n. 1, p. 57-65 | en_HK |
dc.identifier.issn | 0927-7765 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/92475 | - |
dc.description.abstract | In this work, we use two vertically-coupled square two-dimensional lattices to simulate membrane bilayers containing a uniform size distribution of cholesterol immiscible domains of a predetermined size distribution. We substitute cholesterols and phospholipids with their fluorescent analogs and calculate the efficiency of energy transfer as a function of acceptor concentration for four membrane configurations. The simulated efficiency of energy transfer as a function of acceptor concentration data is then fit with an analytical FRET model to estimate the domain size, in the same manner in which experimental FRET data is analyzed. The fitted model parameters (domain size and donor partition coefficient) are compared to the simulation inputs to test the applicability of the FRET model to estimating the size of laterally phase separated cholesterol domains. We show that the FRET model yields good size estimates for domains that range between 1 and 25nm. We also find that the assumed fluorophore configuration in the FRET model leads to a constant under-prediction of these values. Finally, we demonstrate that when two parameters are open to the fit, the FRET model adequately predicts the donor partition coefficient in addition to the domain size. © 2003 Elsevier B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/colsurfb | en_HK |
dc.relation.ispartof | Colloids and Surfaces B: Biointerfaces | en_HK |
dc.subject | Bilayer | en_HK |
dc.subject | Cholesterol | en_HK |
dc.subject | Domains | en_HK |
dc.subject | FRET | en_HK |
dc.subject | Membranes | en_HK |
dc.title | Estimating the size of laterally phase separated cholesterol domains in model membranes with Förster resonance energy transfer: A simulation study | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lee, SP: sumlee@hku.hk | en_HK |
dc.identifier.authority | Lee, SP=rp01351 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.colsurfb.2003.08.016 | en_HK |
dc.identifier.scopus | eid_2-s2.0-0347359169 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0347359169&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 33 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 57 | en_HK |
dc.identifier.epage | 65 | en_HK |
dc.identifier.isi | WOS:000188512400009 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Troup, GM=7005997903 | en_HK |
dc.identifier.scopusauthorid | Tulenko, TN=7005077545 | en_HK |
dc.identifier.scopusauthorid | Lee, SP=7601417497 | en_HK |
dc.identifier.scopusauthorid | Wrenn, SP=6603940041 | en_HK |
dc.identifier.issnl | 0927-7765 | - |