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Conference Paper: Infection efficiency of rat chondrocytes using different hybrids of recombinant adeno-associated virus vectors
Title | Infection efficiency of rat chondrocytes using different hybrids of recombinant adeno-associated virus vectors |
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Authors | |
Issue Date | 2006 |
Publisher | Oxford University Press |
Citation | The 82nd Congress of the European Orthodontic Society, Vienna, Austria, 4 – 8 July 2006. In The European Journal of Orthodontics, 2006, v. 28 n. 6, p. e252-e253 Abstract no.277 How to Cite? |
Abstract | AIM: To investigate the best serotype of recombinant adeno-associated virus (rAAV) that leads to optimum chondrocyte
infection, in an endeavour to create an appropriate delivery system for future gene therapy to regulate condylar growth.
MATERIALS AND METHOD: rAAV2/1, rAAV2/2, rAAV2/5, rAAV2/6, and rAAV2/8 vectors expressing eGFP under
the control of CAG (cytomegalovirus enhancer plus chicken β-actin) promoter were used to infect primary chondrocytes
(isolated from rat mandibular condyles) at a multiplicity of infection of 5 × 104. The infection effi ciency for these cells with
different hybrids was identifi ed using fl uorescence microscopy and fl ow cytometric analysis.
RESULTS: All of the selected serotypes of RAAV infected rat chondrocytes and gave rise to GFP expression. The infection
effi ciency was RAAV2/2 > RAAV2/5 > RAAV2/1 > RAAV2/8 = RAAV2/6.
CONCLUSIONS: rAAV based vectors are capable of infecting rat chondrocytes. rAAV2/2 is the best serotype to infect
chondrocytes in vitro and might thus be of interest for direct gene delivery to condylar cartilage in vivo. |
Persistent Identifier | http://hdl.handle.net/10722/94694 |
ISSN | 2023 Impact Factor: 2.8 2023 SCImago Journal Rankings: 0.940 |
DC Field | Value | Language |
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dc.contributor.author | Rabie, ABM | en_HK |
dc.contributor.author | Dai, J | en_HK |
dc.date.accessioned | 2010-09-25T15:39:05Z | - |
dc.date.available | 2010-09-25T15:39:05Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | The 82nd Congress of the European Orthodontic Society, Vienna, Austria, 4 – 8 July 2006. In The European Journal of Orthodontics, 2006, v. 28 n. 6, p. e252-e253 Abstract no.277 | - |
dc.identifier.issn | 0141-5387 | - |
dc.identifier.uri | http://hdl.handle.net/10722/94694 | - |
dc.description.abstract | AIM: To investigate the best serotype of recombinant adeno-associated virus (rAAV) that leads to optimum chondrocyte infection, in an endeavour to create an appropriate delivery system for future gene therapy to regulate condylar growth. MATERIALS AND METHOD: rAAV2/1, rAAV2/2, rAAV2/5, rAAV2/6, and rAAV2/8 vectors expressing eGFP under the control of CAG (cytomegalovirus enhancer plus chicken β-actin) promoter were used to infect primary chondrocytes (isolated from rat mandibular condyles) at a multiplicity of infection of 5 × 104. The infection effi ciency for these cells with different hybrids was identifi ed using fl uorescence microscopy and fl ow cytometric analysis. RESULTS: All of the selected serotypes of RAAV infected rat chondrocytes and gave rise to GFP expression. The infection effi ciency was RAAV2/2 > RAAV2/5 > RAAV2/1 > RAAV2/8 = RAAV2/6. CONCLUSIONS: rAAV based vectors are capable of infecting rat chondrocytes. rAAV2/2 is the best serotype to infect chondrocytes in vitro and might thus be of interest for direct gene delivery to condylar cartilage in vivo. | - |
dc.language | eng | en_HK |
dc.publisher | Oxford University Press | - |
dc.relation.ispartof | The European Journal of Orthodontics | en_HK |
dc.title | Infection efficiency of rat chondrocytes using different hybrids of recombinant adeno-associated virus vectors | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.email | Rabie, ABM: rabie@hkusua.hku.hk | en_HK |
dc.identifier.authority | Rabie, ABM=rp00029 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1093/ejo/cjl095 | - |
dc.identifier.hkuros | 125810 | en_HK |
dc.identifier.issnl | 0141-5387 | - |