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Conference Paper: Transcriptional profiling of enteric neural crest cells
Title | Transcriptional profiling of enteric neural crest cells |
---|---|
Authors | |
Issue Date | 2005 |
Publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modo |
Citation | The 15th Congress of the International Society of Developmental Biologist (ISDB 2005), Sydney, Australia, 3-7 September 2005. In Mechanisms of Development, 2005, v. 122 suppl. 1, p. S179, abstract no. 15-P016 How to Cite? |
Abstract | To elucidate the molecular mechanisms mediating the stemness of neural
crest cells (NCCs), we have isolated the NCCs from the mouse embryonic
gut (E11.5) and cultured in form of neurospheres. The cultured NCCs are
self-renewable and multipotent. RT-PCR analysis showed that they express
various transcription factor genes characteristic of embryonic NCCs,
including slug, snail, twist and Pax3. Nevertheless, the two master stemness
regulators of embryonic stem cells, Nanog and Oct3/4, were not detected in
the NCCs.
Sonic hedgehog (Shh) was found to promote the proliferation and inhibit
the glial cell line-derived neurotrophic factor (GDNF) induced differentiation
of NCCs. With these mitogenic and neurotrophic factors, NCCs are
fated to proliferate and differentiate, respectively. In attempt to study the
transcriptional profiles of these fated NCCs, we have performed cDNA
microarray analysis. Transcripts of 533 and 454 genes were changed in
response to the GDNF and Shh treatments, respectively, when compared to
the untreated control. They included neuronal markers, signaling,
transcription/translation regulators, cell cycle related genes and expression
sequence tag (EST) genes whose functions are unknown. In the subsequent
data comparison study, we found that 50 genes, including 22% EST (11
genes), show opposite response to GDNF and Shh, of 2 to 24 folds
difference at the transcriptional level. These genes may be implicated in the
maintenance of the stemness of NCCs. Therefore, identification of the
genes that govern proliferation and differentiation of NCCs would provide a
means to reveal the stemness regulator(s) for NCCs. |
Description | Poster abstract |
Persistent Identifier | http://hdl.handle.net/10722/96246 |
ISSN | 2021 Impact Factor: 1.810 2020 SCImago Journal Rankings: 0.890 |
DC Field | Value | Language |
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dc.contributor.author | Ngan, ESW | en_HK |
dc.contributor.author | Lui, VCH | en_HK |
dc.contributor.author | Lau, KC | en_HK |
dc.contributor.author | Garcia-Barcelo, MM | en_HK |
dc.contributor.author | Sham, MH | en_HK |
dc.contributor.author | Tam, PKH | en_HK |
dc.date.accessioned | 2010-09-25T16:27:53Z | - |
dc.date.available | 2010-09-25T16:27:53Z | - |
dc.date.issued | 2005 | en_HK |
dc.identifier.citation | The 15th Congress of the International Society of Developmental Biologist (ISDB 2005), Sydney, Australia, 3-7 September 2005. In Mechanisms of Development, 2005, v. 122 suppl. 1, p. S179, abstract no. 15-P016 | - |
dc.identifier.issn | 0925-4773 | - |
dc.identifier.uri | http://hdl.handle.net/10722/96246 | - |
dc.description | Poster abstract | - |
dc.description.abstract | To elucidate the molecular mechanisms mediating the stemness of neural crest cells (NCCs), we have isolated the NCCs from the mouse embryonic gut (E11.5) and cultured in form of neurospheres. The cultured NCCs are self-renewable and multipotent. RT-PCR analysis showed that they express various transcription factor genes characteristic of embryonic NCCs, including slug, snail, twist and Pax3. Nevertheless, the two master stemness regulators of embryonic stem cells, Nanog and Oct3/4, were not detected in the NCCs. Sonic hedgehog (Shh) was found to promote the proliferation and inhibit the glial cell line-derived neurotrophic factor (GDNF) induced differentiation of NCCs. With these mitogenic and neurotrophic factors, NCCs are fated to proliferate and differentiate, respectively. In attempt to study the transcriptional profiles of these fated NCCs, we have performed cDNA microarray analysis. Transcripts of 533 and 454 genes were changed in response to the GDNF and Shh treatments, respectively, when compared to the untreated control. They included neuronal markers, signaling, transcription/translation regulators, cell cycle related genes and expression sequence tag (EST) genes whose functions are unknown. In the subsequent data comparison study, we found that 50 genes, including 22% EST (11 genes), show opposite response to GDNF and Shh, of 2 to 24 folds difference at the transcriptional level. These genes may be implicated in the maintenance of the stemness of NCCs. Therefore, identification of the genes that govern proliferation and differentiation of NCCs would provide a means to reveal the stemness regulator(s) for NCCs. | - |
dc.language | eng | en_HK |
dc.publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/modo | - |
dc.relation.ispartof | Mechanisms of Development | en_HK |
dc.rights | Mechanisms of Development. Copyright © Elsevier Ireland Ltd. | - |
dc.title | Transcriptional profiling of enteric neural crest cells | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.email | Ngan, ESW: engan@hkucc.hku.hk | en_HK |
dc.identifier.email | Lui, VCH: vchlui@hkucc.hku.hk | en_HK |
dc.identifier.email | Garcia-Barcelo, MM: mmgarcia@hkucc.hku.hk | en_HK |
dc.identifier.email | Sham, MH: mhsham@hkucc.hku.hk | en_HK |
dc.identifier.email | Tam, PKH: paultam@hkucc.hku.hk | en_HK |
dc.identifier.authority | Ngan, ESW=rp00422 | en_HK |
dc.identifier.authority | Lui, VCH=rp00363 | en_HK |
dc.identifier.authority | Garcia-Barcelo, MM=rp00445 | en_HK |
dc.identifier.authority | Sham, MH=rp00380 | en_HK |
dc.identifier.authority | Tam, PKH=rp00060 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.mod.2005.06.010 | - |
dc.identifier.hkuros | 107110 | en_HK |
dc.identifier.hkuros | 107079 | - |
dc.identifier.volume | 122 | - |
dc.identifier.issue | suppl. 1 | - |
dc.identifier.spage | S179, abstract no. 15-P016 | - |
dc.identifier.epage | S179, abstract no. 15-P016 | - |
dc.publisher.place | United Kingdom | - |
dc.description.other | 15th International Society of Developmental Biologist Congress 2005, Sydney, Australia, 3-7 September 2005, In Mechanisms of Development, 2005, v. 122, Suppl. 1, p. S179, abstract no. 15-P016 | - |
dc.identifier.issnl | 0925-4773 | - |