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- PMID: 12707266
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Article: Solution structure of the C-terminal domain of the ciliary neurotrophic factor (CNTF) receptor and ligand free associations among components of the CNTF receptor complex
Title | Solution structure of the C-terminal domain of the ciliary neurotrophic factor (CNTF) receptor and ligand free associations among components of the CNTF receptor complex |
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Authors | |
Issue Date | 2003 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ |
Citation | Journal Of Biological Chemistry, 2003, v. 278 n. 26, p. 23285-23294 How to Cite? |
Abstract | The functional receptor complex of ciliary neurotrophic factor (CNTF), a member of the gp130 family of cytokines, is composed of CNTF, the CNTF receptor a (CNTFR), gp130, and the leukemia inhibitory factor receptor (LIFR). However, the nature of the receptor-mediated interactions in this complex has not yet been resolved. To address this issue we have determined the solution structure of the C-terminal or BC domain of CNTFR and studied the interactions of CNTFR with LIFR and gp130. We reported previously that the membrane distal cytokine-binding domain (CBD1) of LIFR could interact in vitro with soluble CNTFR (sCNTFR) in the absence of CNTF. Here we show that the CBD of human gp130 can also bind in vitro to sCNTFR in the absence of CNTF. In addition, the gp130 CBD could compete with the LIFR CBD1 for the binding of sCNTFR. Substitution of residues in the gp130 CBD, the LIFR CBD1, and the CNTFR BC domain that are expected to be involved in receptor-receptor interactions significantly reduced their interactions. An NMR chemical shift perturbation study of the interaction between the BC domains of CNTFR and gp130 further mapped the interaction surface. These data suggest that both the gp130 CBD and the LIFR CBD1 interact with CNTFR in a similar way and provide insights into the nature of the CNTF receptor complex. |
Persistent Identifier | http://hdl.handle.net/10722/96915 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Man, D | en_HK |
dc.contributor.author | He, W | en_HK |
dc.contributor.author | Sze, KH | en_HK |
dc.contributor.author | Gong, K | en_HK |
dc.contributor.author | Smith, DK | en_HK |
dc.contributor.author | Zhu, G | en_HK |
dc.contributor.author | Ip, NY | en_HK |
dc.date.accessioned | 2010-09-25T16:49:41Z | - |
dc.date.available | 2010-09-25T16:49:41Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | Journal Of Biological Chemistry, 2003, v. 278 n. 26, p. 23285-23294 | en_HK |
dc.identifier.issn | 0021-9258 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/96915 | - |
dc.description.abstract | The functional receptor complex of ciliary neurotrophic factor (CNTF), a member of the gp130 family of cytokines, is composed of CNTF, the CNTF receptor a (CNTFR), gp130, and the leukemia inhibitory factor receptor (LIFR). However, the nature of the receptor-mediated interactions in this complex has not yet been resolved. To address this issue we have determined the solution structure of the C-terminal or BC domain of CNTFR and studied the interactions of CNTFR with LIFR and gp130. We reported previously that the membrane distal cytokine-binding domain (CBD1) of LIFR could interact in vitro with soluble CNTFR (sCNTFR) in the absence of CNTF. Here we show that the CBD of human gp130 can also bind in vitro to sCNTFR in the absence of CNTF. In addition, the gp130 CBD could compete with the LIFR CBD1 for the binding of sCNTFR. Substitution of residues in the gp130 CBD, the LIFR CBD1, and the CNTFR BC domain that are expected to be involved in receptor-receptor interactions significantly reduced their interactions. An NMR chemical shift perturbation study of the interaction between the BC domains of CNTFR and gp130 further mapped the interaction surface. These data suggest that both the gp130 CBD and the LIFR CBD1 interact with CNTFR in a similar way and provide insights into the nature of the CNTF receptor complex. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_HK |
dc.relation.ispartof | Journal of Biological Chemistry | en_HK |
dc.subject.mesh | Amino Acid Substitution | en_HK |
dc.subject.mesh | Antigens, CD - chemistry - metabolism | en_HK |
dc.subject.mesh | Binding Sites | en_HK |
dc.subject.mesh | Cytokine Receptor gp130 | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Leukemia Inhibitory Factor Receptor alpha Subunit | en_HK |
dc.subject.mesh | Membrane Glycoproteins - chemistry - metabolism | en_HK |
dc.subject.mesh | Models, Molecular | en_HK |
dc.subject.mesh | Nuclear Magnetic Resonance, Biomolecular | en_HK |
dc.subject.mesh | Protein Binding | en_HK |
dc.subject.mesh | Protein Structure, Tertiary | en_HK |
dc.subject.mesh | Receptor, Ciliary Neurotrophic Factor - chemistry - genetics - metabolism | en_HK |
dc.subject.mesh | Receptors, Cytokine - chemistry - metabolism | en_HK |
dc.subject.mesh | Receptors, OSM-LIF | en_HK |
dc.subject.mesh | Solutions | en_HK |
dc.title | Solution structure of the C-terminal domain of the ciliary neurotrophic factor (CNTF) receptor and ligand free associations among components of the CNTF receptor complex | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Sze, KH:khsze@hku.hk | en_HK |
dc.identifier.authority | Sze, KH=rp00785 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1074/jbc.M301976200 | en_HK |
dc.identifier.pmid | 12707266 | - |
dc.identifier.scopus | eid_2-s2.0-0038607650 | en_HK |
dc.identifier.hkuros | 93916 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0038607650&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 278 | en_HK |
dc.identifier.issue | 26 | en_HK |
dc.identifier.spage | 23285 | en_HK |
dc.identifier.epage | 23294 | en_HK |
dc.identifier.isi | WOS:000183638600011 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Man, D=36878997300 | en_HK |
dc.identifier.scopusauthorid | He, W=55218918100 | en_HK |
dc.identifier.scopusauthorid | Sze, KH=7006735061 | en_HK |
dc.identifier.scopusauthorid | Gong, K=36900598600 | en_HK |
dc.identifier.scopusauthorid | Smith, DK=7410351143 | en_HK |
dc.identifier.scopusauthorid | Zhu, G=7402633110 | en_HK |
dc.identifier.scopusauthorid | Ip, NY=7005756760 | en_HK |
dc.identifier.issnl | 0021-9258 | - |