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Article: 14-3-3 Protein regulates cell adhesion in the seminiferous epithelium of rat testes
Title | 14-3-3 Protein regulates cell adhesion in the seminiferous epithelium of rat testes | ||||||
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Authors | |||||||
Issue Date | 2009 | ||||||
Publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org | ||||||
Citation | Endocrinology, 2009, v. 150 n. 10, p. 4713-4723 How to Cite? | ||||||
Abstract | Polarity proteins have been implicated in regulating and maintaining tight junction (TJ) and cell polarity in epithelia. Here we report 14-3-3θ, the homolog of Caenorhabditis elegans Par5 in mammalian cells, which is known to confer cell polarity at TJ, is found at the apical ectoplasmic specialization (ES), a testis-specific adherens junction type restricted to the Sertoli cell-elongating spermatid interface, in which TJ is absent. 14-3-3θ was shown to play a critical role in conferring cell adhesion at the apical ES. A loss of 14-3-3θ expression at the apical ES was detected in the seminiferous epithelium before spermiation. Involvement of 14-3-3θ in Sertoli cell adhesion was confirmed by its knockdown by RNA interference in Sertoli cells cultured in vitro with established TJ permeability barrier that mimicked the blood-testis barrier (BTB) in vivo. Mislocalization of N-cadherin and zonula occludens-1, but not α- and β-catenins, was observed after 14-3-3θ knockdown in Sertoli cells, moving from the cell-cell interface to cytosol, indicating a disruption of cell adhesion. Studies by endocytosis assay illustrated that this loss of cell adhesion was mediated by an increase in the kinetics of endocytosis of N-cadherin and junctional adhesion molecule-A at the BTB, which may represent a general mechanism by which polarity proteins regulate cell adhesion. In summary, the testis is using 14-3-3θ to regulate cell adhesion at the apical ES to facilitate spermiation and at the BTB to facilitate the transit of preleptotene spermatocytes at stages VIII-IX of the epithelial cycle. 14-3-3θ may act as a molecular switch that coordinates these two cellular events in the seminiferous epithelium during spermatogenesis. Copyright © 2009 by The Endocrine Society. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/127426 | ||||||
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 1.285 | ||||||
PubMed Central ID | |||||||
ISI Accession Number ID |
Funding Information: Disclosure Summary: The authors have nothing to disclose. | ||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, EWP | en_HK |
dc.contributor.author | Sun, S | en_HK |
dc.contributor.author | Li, MWM | en_HK |
dc.contributor.author | Lee, WM | en_HK |
dc.contributor.author | Cheng, CY | en_HK |
dc.date.accessioned | 2010-10-31T13:24:56Z | - |
dc.date.available | 2010-10-31T13:24:56Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Endocrinology, 2009, v. 150 n. 10, p. 4713-4723 | en_HK |
dc.identifier.issn | 0013-7227 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/127426 | - |
dc.description.abstract | Polarity proteins have been implicated in regulating and maintaining tight junction (TJ) and cell polarity in epithelia. Here we report 14-3-3θ, the homolog of Caenorhabditis elegans Par5 in mammalian cells, which is known to confer cell polarity at TJ, is found at the apical ectoplasmic specialization (ES), a testis-specific adherens junction type restricted to the Sertoli cell-elongating spermatid interface, in which TJ is absent. 14-3-3θ was shown to play a critical role in conferring cell adhesion at the apical ES. A loss of 14-3-3θ expression at the apical ES was detected in the seminiferous epithelium before spermiation. Involvement of 14-3-3θ in Sertoli cell adhesion was confirmed by its knockdown by RNA interference in Sertoli cells cultured in vitro with established TJ permeability barrier that mimicked the blood-testis barrier (BTB) in vivo. Mislocalization of N-cadherin and zonula occludens-1, but not α- and β-catenins, was observed after 14-3-3θ knockdown in Sertoli cells, moving from the cell-cell interface to cytosol, indicating a disruption of cell adhesion. Studies by endocytosis assay illustrated that this loss of cell adhesion was mediated by an increase in the kinetics of endocytosis of N-cadherin and junctional adhesion molecule-A at the BTB, which may represent a general mechanism by which polarity proteins regulate cell adhesion. In summary, the testis is using 14-3-3θ to regulate cell adhesion at the apical ES to facilitate spermiation and at the BTB to facilitate the transit of preleptotene spermatocytes at stages VIII-IX of the epithelial cycle. 14-3-3θ may act as a molecular switch that coordinates these two cellular events in the seminiferous epithelium during spermatogenesis. Copyright © 2009 by The Endocrine Society. | en_HK |
dc.language | eng | en_HK |
dc.publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org | en_HK |
dc.relation.ispartof | Endocrinology | en_HK |
dc.subject.mesh | 14-3-3 Proteins - metabolism | - |
dc.subject.mesh | Blood-Testis Barrier - physiology | - |
dc.subject.mesh | Cell Adhesion | - |
dc.subject.mesh | Endocytosis | - |
dc.subject.mesh | Seminiferous Epithelium - physiology | - |
dc.title | 14-3-3 Protein regulates cell adhesion in the seminiferous epithelium of rat testes | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=150&issue=10&spage=4713&epage=4723&date=2009&atitle=14-3-3+regulates+cell+adhesion+in+the+seminiferous+epithelium | en_HK |
dc.identifier.email | Lee, WM: hrszlwm@hku.hk | en_HK |
dc.identifier.authority | Lee, WM=rp00728 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1210/en.2009-0427 | en_HK |
dc.identifier.pmid | 19608648 | - |
dc.identifier.pmcid | PMC2754685 | - |
dc.identifier.scopus | eid_2-s2.0-70349331263 | en_HK |
dc.identifier.hkuros | 179021 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-70349331263&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 150 | en_HK |
dc.identifier.issue | 10 | en_HK |
dc.identifier.spage | 4713 | en_HK |
dc.identifier.epage | 4723 | en_HK |
dc.identifier.isi | WOS:000270021700026 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Wong, EWP=23029194700 | en_HK |
dc.identifier.scopusauthorid | Sun, S=34977782600 | en_HK |
dc.identifier.scopusauthorid | Li, MWM=27168276300 | en_HK |
dc.identifier.scopusauthorid | Lee, WM=24799156600 | en_HK |
dc.identifier.scopusauthorid | Cheng, CY=7404797787 | en_HK |
dc.identifier.issnl | 0013-7227 | - |