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Article: Proteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HepG2
Title | Proteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HepG2 | ||||||||
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Authors | |||||||||
Keywords | Astragaloside IV Chemoprevention Gene regulation Oncogene Vav3 Tumorigenesis | ||||||||
Issue Date | 2010 | ||||||||
Publisher | Spandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/ | ||||||||
Citation | International Journal Of Oncology, 2010, v. 36 n. 3, p. 725-735 How to Cite? | ||||||||
Abstract | Triterpenoids are implicated in the chemoprevention of various cancers. Current challenge is to define the molecular mechanism underlying the chemopreventive activity of triterpenoids. This study was designed to characterize the intracellular proteins regulated by astragaloside IV, the major active triterpenoid in Radix Astragali. Upon the treatment with astragaloside IV, human hepatocellular carcinoma HepG2 cells were evaluated for the colonogenic survival and anchorage-independent growth. The cellular proteins of treated and untreated cells were resolved by 2-D polyacrylamide gel electrophoresis. The protein spots mostly altered by drug treatment were identified by mass spectrometry and subsequently verified by Western blotting using specific antibodies and RT-PCR technique using specific DNA primers. We found that astragaloside IV attenuated the colonogenic survival and anchorage-independent growth of cancer cells. Based on the proteomic profiles, top 14 upregulated and 13 down-regulated protein spots were subjected to mass spectrometric analysis. As an example, Vav3.1 belongs to the oncogene Vav family, which is implicated in tumorigenesis. Vav3.1 expression was down-regulated by astragaloside IV in a dose- and time-dependent manner. Down-regulation of Vav3.1 was highly correlated with the suppression of cell malignant transform. Thus, astragaloside IV may elicit anticancer activity via down-regulating the expression of oncogenes such as Vav3.1. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/127602 | ||||||||
ISSN | 2023 Impact Factor: 4.5 2023 SCImago Journal Rankings: 1.099 | ||||||||
ISI Accession Number ID |
Funding Information: We acknowledge Miss Yim-Hing Cheung for her excellent technical assistance. We also thank Dr Priscilla Leung and Mr Lawrence Luk from Genome Research Centre, University of Hong Kong, for their technical assistance with 2D SDSPAGE analysis and protein identification by MALDI/TOF mass spectrometry. This work was supported by the Dean's Fund for Traditional Chinese Medicine Program, Li Ka Shing Faculty of Medicine, and the Seed Funding for Basic Research, The University of Hong Kong. | ||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Qi, H | en_HK |
dc.contributor.author | Wei, L | en_HK |
dc.contributor.author | Han, Y | en_HK |
dc.contributor.author | Zhang, Q | en_HK |
dc.contributor.author | Lau, ASY | en_HK |
dc.contributor.author | Rong, J | en_HK |
dc.date.accessioned | 2010-10-31T13:35:03Z | - |
dc.date.available | 2010-10-31T13:35:03Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | International Journal Of Oncology, 2010, v. 36 n. 3, p. 725-735 | en_HK |
dc.identifier.issn | 1019-6439 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/127602 | - |
dc.description.abstract | Triterpenoids are implicated in the chemoprevention of various cancers. Current challenge is to define the molecular mechanism underlying the chemopreventive activity of triterpenoids. This study was designed to characterize the intracellular proteins regulated by astragaloside IV, the major active triterpenoid in Radix Astragali. Upon the treatment with astragaloside IV, human hepatocellular carcinoma HepG2 cells were evaluated for the colonogenic survival and anchorage-independent growth. The cellular proteins of treated and untreated cells were resolved by 2-D polyacrylamide gel electrophoresis. The protein spots mostly altered by drug treatment were identified by mass spectrometry and subsequently verified by Western blotting using specific antibodies and RT-PCR technique using specific DNA primers. We found that astragaloside IV attenuated the colonogenic survival and anchorage-independent growth of cancer cells. Based on the proteomic profiles, top 14 upregulated and 13 down-regulated protein spots were subjected to mass spectrometric analysis. As an example, Vav3.1 belongs to the oncogene Vav family, which is implicated in tumorigenesis. Vav3.1 expression was down-regulated by astragaloside IV in a dose- and time-dependent manner. Down-regulation of Vav3.1 was highly correlated with the suppression of cell malignant transform. Thus, astragaloside IV may elicit anticancer activity via down-regulating the expression of oncogenes such as Vav3.1. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Spandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/ | en_HK |
dc.relation.ispartof | International Journal of Oncology | en_HK |
dc.subject | Astragaloside IV | en_HK |
dc.subject | Chemoprevention | en_HK |
dc.subject | Gene regulation | en_HK |
dc.subject | Oncogene Vav3 | en_HK |
dc.subject | Tumorigenesis | en_HK |
dc.subject.mesh | Carcinoma, Hepatocellular - drug therapy | - |
dc.subject.mesh | Proteomics - methods | - |
dc.subject.mesh | Saponins - pharmacology | - |
dc.subject.mesh | Triterpenes - pharmacology | - |
dc.subject.mesh | Triterpenes - pharmacology | - |
dc.title | Proteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HepG2 | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1019-6439&volume=36&issue=3&spage=725&epage=735&date=2010&atitle=Proteomic+characterization+of+the+cellular+response+to+chemopreventive+triterpenoid+astragaloside+IV+in+human+hepatocelluar+carcinoma+cell+line+HepG2 | - |
dc.identifier.email | Lau, ASY: asylau@hku.hk | en_HK |
dc.identifier.email | Rong, J: jrong@hku.hk | en_HK |
dc.identifier.authority | Lau, ASY=rp00474 | en_HK |
dc.identifier.authority | Rong, J=rp00515 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3892/ijo-00000548 | en_HK |
dc.identifier.pmid | 20126993 | - |
dc.identifier.scopus | eid_2-s2.0-77149121747 | en_HK |
dc.identifier.hkuros | 179325 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77149121747&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 36 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 725 | en_HK |
dc.identifier.epage | 735 | en_HK |
dc.identifier.isi | WOS:000274718400023 | - |
dc.publisher.place | Greece | en_HK |
dc.identifier.scopusauthorid | Qi, H=35367105300 | en_HK |
dc.identifier.scopusauthorid | Wei, L=43861929300 | en_HK |
dc.identifier.scopusauthorid | Han, Y=8527680500 | en_HK |
dc.identifier.scopusauthorid | Zhang, Q=12345014000 | en_HK |
dc.identifier.scopusauthorid | Lau, ASY=7202626202 | en_HK |
dc.identifier.scopusauthorid | Rong, J=7005980047 | en_HK |
dc.identifier.issnl | 1019-6439 | - |