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- Scopus: eid_2-s2.0-0024287709
- PMID: 3421893
- WOS: WOS:A1988N614300001
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Article: Inositol 1:2(cyclic),4,5-trisphosphate is not a major product of inositol phospholipid metabolism in vasopressin-stimulated WRK1 cells.
Title | Inositol 1:2(cyclic),4,5-trisphosphate is not a major product of inositol phospholipid metabolism in vasopressin-stimulated WRK1 cells. |
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Authors | |
Issue Date | 1988 |
Publisher | Portland Press Ltd. The Journal's web site is located at http://www.biochemj.org |
Citation | Biochemical Journal, 1988, v. 252 n. 1, p. 1-5 How to Cite? |
Abstract | 1. A method has been devised for quenching cell incubations with an aqueous phenol/chloroform/EDTA mixture of neutral pH, to allow the analysis of acid-labile cell components. 2. Using this method, we have searched for the appearance of Ins(1:2cyclic,4,5)P3 [inositol 1:2(cyclic),4,5-trisphosphate] in WRK1 mammary tumour cells that were labelled to high specific radioactivity with [3H]inositol and then stimulated with 0.4 microM-vasopressin. 3. Vasopressin caused a very rapid accumulation of Ins(1,4,5)P3 (inositol 1,4,5-trisphosphate), followed by a slower decline towards the original concentration. An acid-labile and inositol-labelled compound with the chromatographic properties of Ins(1:2cyclic,4,5)P3 was present in unstimulated cells at less than 5% of the elevated concentration of Ins(1,4,5)P3. Its concentration rose 2-3-fold during stimulation for 3 min, at which time its concentration was about 5% of the elevated concentration of Ins(1,4,5)P3. 4. We conclude that Ins(1,4,5)P3 is the major product of phosphoinositidase C-catalysed phosphatidylinositol 4,5-bisphosphate hydrolysis in vasopressin-stimulated WRK1 cells. Ins(1:2cyclic,4,5)P3 is unlikely to be an important intracellular messenger in these cells, at least during the first few minutes of stimulation. |
Persistent Identifier | http://hdl.handle.net/10722/147331 |
ISSN | 2021 Impact Factor: 3.766 2020 SCImago Journal Rankings: 1.706 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wong, NS | en_US |
dc.contributor.author | Barker, CJ | en_US |
dc.contributor.author | Shears, SB | en_US |
dc.contributor.author | Kirk, CJ | en_US |
dc.contributor.author | Michell, RH | en_US |
dc.date.accessioned | 2012-05-29T06:02:58Z | - |
dc.date.available | 2012-05-29T06:02:58Z | - |
dc.date.issued | 1988 | en_US |
dc.identifier.citation | Biochemical Journal, 1988, v. 252 n. 1, p. 1-5 | en_US |
dc.identifier.issn | 0264-6021 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147331 | - |
dc.description.abstract | 1. A method has been devised for quenching cell incubations with an aqueous phenol/chloroform/EDTA mixture of neutral pH, to allow the analysis of acid-labile cell components. 2. Using this method, we have searched for the appearance of Ins(1:2cyclic,4,5)P3 [inositol 1:2(cyclic),4,5-trisphosphate] in WRK1 mammary tumour cells that were labelled to high specific radioactivity with [3H]inositol and then stimulated with 0.4 microM-vasopressin. 3. Vasopressin caused a very rapid accumulation of Ins(1,4,5)P3 (inositol 1,4,5-trisphosphate), followed by a slower decline towards the original concentration. An acid-labile and inositol-labelled compound with the chromatographic properties of Ins(1:2cyclic,4,5)P3 was present in unstimulated cells at less than 5% of the elevated concentration of Ins(1,4,5)P3. Its concentration rose 2-3-fold during stimulation for 3 min, at which time its concentration was about 5% of the elevated concentration of Ins(1,4,5)P3. 4. We conclude that Ins(1,4,5)P3 is the major product of phosphoinositidase C-catalysed phosphatidylinositol 4,5-bisphosphate hydrolysis in vasopressin-stimulated WRK1 cells. Ins(1:2cyclic,4,5)P3 is unlikely to be an important intracellular messenger in these cells, at least during the first few minutes of stimulation. | en_US |
dc.language | eng | en_US |
dc.publisher | Portland Press Ltd. The Journal's web site is located at http://www.biochemj.org | en_US |
dc.relation.ispartof | Biochemical Journal | en_US |
dc.subject.mesh | Arginine Vasopressin - Pharmacology | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Chromatography, High Pressure Liquid | en_US |
dc.subject.mesh | Inositol Phosphates - Metabolism | en_US |
dc.subject.mesh | Phosphatidylinositols - Metabolism | en_US |
dc.subject.mesh | Sugar Phosphates - Metabolism | en_US |
dc.title | Inositol 1:2(cyclic),4,5-trisphosphate is not a major product of inositol phospholipid metabolism in vasopressin-stimulated WRK1 cells. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wong, NS:nswong@hkucc.hku.hk | en_US |
dc.identifier.authority | Wong, NS=rp00340 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 3421893 | - |
dc.identifier.scopus | eid_2-s2.0-0024287709 | en_US |
dc.identifier.volume | 252 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 1 | en_US |
dc.identifier.epage | 5 | en_US |
dc.identifier.isi | WOS:A1988N614300001 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Wong, NS=7202836641 | en_US |
dc.identifier.scopusauthorid | Barker, CJ=7201818641 | en_US |
dc.identifier.scopusauthorid | Shears, SB=7007089039 | en_US |
dc.identifier.scopusauthorid | Kirk, CJ=7102433740 | en_US |
dc.identifier.scopusauthorid | Michell, RH=7006966596 | en_US |
dc.identifier.issnl | 0264-6021 | - |