File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1074/jbc.M603417200
- Scopus: eid_2-s2.0-33750043186
- PMID: 16914552
- WOS: WOS:000241075900089
- Find via
Supplementary
-
Bookmarks:
- CiteULike: 1
- Citations:
- Appears in Collections:
Article: A dominant function of IKK/NF-κB signaling in global lipopolysaccharide-induced gene expression
Title | A dominant function of IKK/NF-κB signaling in global lipopolysaccharide-induced gene expression |
---|---|
Authors | |
Issue Date | 2006 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ |
Citation | Journal Of Biological Chemistry, 2006, v. 281 n. 41, p. 31142-31151 How to Cite? |
Abstract | Porphyromonas gingivalis is an etiologic pathogen of periodontitis that is one of the most common inflammatory diseases. Recently, we found that P. gingivalis LPS activated the transcription factor nuclear factor-κB (NF-κB) through the IκB kinase complex (IKK). NF-κB is a transcription factor that controls inflammation and host responses. In this study, we examined the role of IKK/NF-κB in P. gingivalis LPS-induced gene expression on a genome-wide basis using a combination of microarray and biochemical approaches. A total of 88 early response genes were found to be induced by P. gingivalis LPS in a human THP.1 monocytic cell lines. Interestingly, the induction of most of these genes was abolished or attenuated under the inactivation of IKK/NF-κB. Among those IKK/NF-κB-dependent genes, 20 genes were NF-κB-inducible genes reported previously, and 59 genes represented putative novel NF-κB target genes. Using transcription factor binding analysis, we found that most of these putative NF-κB target genes contained one or multiple NF-κB-binding sites. Also, some transcription factor-binding motifs were overrepresented in the promoter of both known and putative NF-κB-dependent genes, indicating that these genes may be regulated in a similar fashion. Furthermore, we found that several transcription factors associated with metabolic and inflammatory responses, including nuclear receptors, activator of protein-1, and early growth responses, were induced by P. gingivalis LPS through IKK/NF-κB, indicating that IKK/NF-κB may utilize these transcription factors to mediate secondary responses. Taken together, our results demonstrate that IKK/NF-κB signaling plays a dominant role in P. gingivalis LPS-induced early response gene expression, suggesting that IKK/NF-κB is a therapeutic target for periodontitis. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/154429 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Carayol, N | en_US |
dc.contributor.author | Chen, J | en_US |
dc.contributor.author | Yang, F | en_US |
dc.contributor.author | Jin, T | en_US |
dc.contributor.author | Jin, L | en_US |
dc.contributor.author | States, D | en_US |
dc.contributor.author | Wang, CY | en_US |
dc.date.accessioned | 2012-08-08T08:25:16Z | - |
dc.date.available | 2012-08-08T08:25:16Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Journal Of Biological Chemistry, 2006, v. 281 n. 41, p. 31142-31151 | en_US |
dc.identifier.issn | 0021-9258 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/154429 | - |
dc.description.abstract | Porphyromonas gingivalis is an etiologic pathogen of periodontitis that is one of the most common inflammatory diseases. Recently, we found that P. gingivalis LPS activated the transcription factor nuclear factor-κB (NF-κB) through the IκB kinase complex (IKK). NF-κB is a transcription factor that controls inflammation and host responses. In this study, we examined the role of IKK/NF-κB in P. gingivalis LPS-induced gene expression on a genome-wide basis using a combination of microarray and biochemical approaches. A total of 88 early response genes were found to be induced by P. gingivalis LPS in a human THP.1 monocytic cell lines. Interestingly, the induction of most of these genes was abolished or attenuated under the inactivation of IKK/NF-κB. Among those IKK/NF-κB-dependent genes, 20 genes were NF-κB-inducible genes reported previously, and 59 genes represented putative novel NF-κB target genes. Using transcription factor binding analysis, we found that most of these putative NF-κB target genes contained one or multiple NF-κB-binding sites. Also, some transcription factor-binding motifs were overrepresented in the promoter of both known and putative NF-κB-dependent genes, indicating that these genes may be regulated in a similar fashion. Furthermore, we found that several transcription factors associated with metabolic and inflammatory responses, including nuclear receptors, activator of protein-1, and early growth responses, were induced by P. gingivalis LPS through IKK/NF-κB, indicating that IKK/NF-κB may utilize these transcription factors to mediate secondary responses. Taken together, our results demonstrate that IKK/NF-κB signaling plays a dominant role in P. gingivalis LPS-induced early response gene expression, suggesting that IKK/NF-κB is a therapeutic target for periodontitis. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_US |
dc.relation.ispartof | Journal of Biological Chemistry | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Gene Expression Regulation | en_US |
dc.subject.mesh | Genes, Dominant | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | I-Kappa B Kinase - Metabolism | en_US |
dc.subject.mesh | Lipopolysaccharides - Metabolism | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Monocytes - Microbiology | en_US |
dc.subject.mesh | Nf-Kappa B - Metabolism | en_US |
dc.subject.mesh | Porphyromonas Gingivalis - Metabolism | en_US |
dc.subject.mesh | Signal Transduction | en_US |
dc.title | A dominant function of IKK/NF-κB signaling in global lipopolysaccharide-induced gene expression | en_US |
dc.type | Article | en_US |
dc.identifier.email | Jin, L:ljjin@hkucc.hku.hk | en_US |
dc.identifier.authority | Jin, L=rp00028 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1074/jbc.M603417200 | en_US |
dc.identifier.pmid | 16914552 | - |
dc.identifier.scopus | eid_2-s2.0-33750043186 | en_US |
dc.identifier.hkuros | 124070 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33750043186&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 281 | en_US |
dc.identifier.issue | 41 | en_US |
dc.identifier.spage | 31142 | en_US |
dc.identifier.epage | 31151 | en_US |
dc.identifier.isi | WOS:000241075900089 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Carayol, N=36009813700 | en_US |
dc.identifier.scopusauthorid | Chen, J=49761227000 | en_US |
dc.identifier.scopusauthorid | Yang, F=54379471900 | en_US |
dc.identifier.scopusauthorid | Jin, T=8527982400 | en_US |
dc.identifier.scopusauthorid | Jin, L=7403328850 | en_US |
dc.identifier.scopusauthorid | States, D=35866621200 | en_US |
dc.identifier.scopusauthorid | Wang, CY=49762557200 | en_US |
dc.identifier.citeulike | 11369876 | - |
dc.identifier.issnl | 0021-9258 | - |