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- Scopus: eid_2-s2.0-44249092609
- PMID: 15605102
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Article: Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro.
Title | Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro. |
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Authors | |
Issue Date | 2004 |
Publisher | Peking University Health Science Center. The Journal's web site is located at http://xuebao.bjmu.edu.cn/ |
Citation | Beijing Da Xue Xue Bao. Yi Xue Ban = Journal Of Peking University. Health Sciences, 2004, v. 36 n. 6, p. 646-649 How to Cite? |
Abstract | OBJECTIVE: To study the expression of osteoprotegerin(OPG) and receptor activator nuclear factor kappa B ligand(RANKL) at protein level in human periodontal ligament cells (HPDLCs), and the effect of 1alpha,25(OH)(2) vitamin D(3) [1,25(OH)(2)vitD(3)] on the secretion of OPG protein in vitro. METHODS: HPDLCs were harvested in vitro by sequential digestion with trypsin and collagenase. The expression of RANKL in HPDLCs at protein level was tested by immunocyto-chemistry. Enzyme-linked immunoadsordent assay (ELISA) was used to detect the OPG protein which was secreted into the culture medium by HPDLCs cultured with and without 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3) on the 0, 2nd, 4th, and 6th days, respectively. RESULTS: RANKL protein was detected on the membrane and plasma of HPDLCs, and OPG protein was secreted in the culture medium. The secretion of OPG protein was down-regulated by 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3). CONCLUSION: HPDLCs have the bone metabolism system of OPG/RANKL, which works during the process of 1alpha,25 (OH)(2)vitD(3) inducing HPDLCs. The conclusion has laid the groundwork for the study on bone remodelling mechanisms of HPDLCs. |
Persistent Identifier | http://hdl.handle.net/10722/154516 |
ISSN | 2023 SCImago Journal Rankings: 0.175 |
DC Field | Value | Language |
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dc.contributor.author | Zhang, D | en_US |
dc.contributor.author | Yang, YQ | en_US |
dc.contributor.author | Li, XT | en_US |
dc.contributor.author | Fu, MK | en_US |
dc.date.accessioned | 2012-08-08T08:25:54Z | - |
dc.date.available | 2012-08-08T08:25:54Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Beijing Da Xue Xue Bao. Yi Xue Ban = Journal Of Peking University. Health Sciences, 2004, v. 36 n. 6, p. 646-649 | en_US |
dc.identifier.issn | 1671-167X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/154516 | - |
dc.description.abstract | OBJECTIVE: To study the expression of osteoprotegerin(OPG) and receptor activator nuclear factor kappa B ligand(RANKL) at protein level in human periodontal ligament cells (HPDLCs), and the effect of 1alpha,25(OH)(2) vitamin D(3) [1,25(OH)(2)vitD(3)] on the secretion of OPG protein in vitro. METHODS: HPDLCs were harvested in vitro by sequential digestion with trypsin and collagenase. The expression of RANKL in HPDLCs at protein level was tested by immunocyto-chemistry. Enzyme-linked immunoadsordent assay (ELISA) was used to detect the OPG protein which was secreted into the culture medium by HPDLCs cultured with and without 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3) on the 0, 2nd, 4th, and 6th days, respectively. RESULTS: RANKL protein was detected on the membrane and plasma of HPDLCs, and OPG protein was secreted in the culture medium. The secretion of OPG protein was down-regulated by 10(-8) mol/L 1alpha,25 (OH)(2)vitD(3). CONCLUSION: HPDLCs have the bone metabolism system of OPG/RANKL, which works during the process of 1alpha,25 (OH)(2)vitD(3) inducing HPDLCs. The conclusion has laid the groundwork for the study on bone remodelling mechanisms of HPDLCs. | en_US |
dc.language | eng | en_US |
dc.publisher | Peking University Health Science Center. The Journal's web site is located at http://xuebao.bjmu.edu.cn/ | en_US |
dc.relation.ispartof | Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences | en_US |
dc.subject.mesh | Calcifediol - Pharmacology | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Immunohistochemistry | en_US |
dc.subject.mesh | Osteoprotegerin - Biosynthesis | en_US |
dc.subject.mesh | Periodontal Ligament - Cytology - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Rank Ligand - Biosynthesis | en_US |
dc.title | Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1alpha,25(OH)(2) vitamin D(3) on the secretion of OPG protein in vitro. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Yang, YQ:yangyanq@hkucc.hku.hk | en_US |
dc.identifier.authority | Yang, YQ=rp00045 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 15605102 | - |
dc.identifier.scopus | eid_2-s2.0-44249092609 | en_US |
dc.identifier.hkuros | 146514 | - |
dc.identifier.volume | 36 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 646 | en_US |
dc.identifier.epage | 649 | en_US |
dc.publisher.place | China | en_US |
dc.identifier.scopusauthorid | Zhang, D=7405356257 | en_US |
dc.identifier.scopusauthorid | Yang, YQ=36623085300 | en_US |
dc.identifier.scopusauthorid | Li, XT=7501710811 | en_US |
dc.identifier.scopusauthorid | Fu, MK=7202031177 | en_US |
dc.identifier.issnl | 1671-167X | - |