File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1182/blood.V93.9.3026
- Scopus: eid_2-s2.0-0033134768
- PMID: 10216099
- WOS: WOS:000079984800032
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Expression of Epstein-Barr virus BamHI-A rightward transcripts in latently infected B cells from peripheral blood
Title | Expression of Epstein-Barr virus BamHI-A rightward transcripts in latently infected B cells from peripheral blood |
---|---|
Authors | |
Issue Date | 1999 |
Publisher | American Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/ |
Citation | Blood, 1999, v. 93 n. 9, p. 3026-3032 How to Cite? |
Abstract | In addition to the Epstein-Barr virus (EBV) EBNA and LMP latency genes, there is a family of alternatively spliced BamHI-A rightward transcripts (BARTs). These latency transcripts are highly expressed in the EBV-associated malignancies nasopharyngeal carcinoma and Burkitt's lymphoma, and are expressed at lower levels in latently EBV-infected B-cell lines. The contribution of the BARTs to EBV biology or pathogenesis is unknown. Resting B cells have recently been recognized as a reservoir for EBV persistence in the peripheral blood. In these cells, EBV gene expression is tightly restricted and the only viral gene known to be consistently expressed is LMP2A. We used cell sorting and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine whether BARTs are expressed in the restricted form of in vivo latency. Our results demonstrated that RNAs with splicing diagnostic for transcripts containing the BART RPMS1 and BARFO open-reading frames (ORFs) were expressed in CD19+ but not in CD23+ B cells isolated from peripheral blood of healthy individuals. The product of the proximal RPMS1 ORF has not previously been characterized. The RPMS1 ORF was shown to encode a 15-kD protein that localized to the nucleus of transfected cells. Expression of the BARTs in peripheral blood B cells suggests that the proteins encoded by these transcripts are likely to be important for maintenance of in vivo latency. |
Persistent Identifier | http://hdl.handle.net/10722/157298 |
ISSN | 2023 Impact Factor: 21.0 2023 SCImago Journal Rankings: 5.272 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, H | en_US |
dc.contributor.author | Smith, P | en_US |
dc.contributor.author | Ambinder, RF | en_US |
dc.contributor.author | Hayward, SD | en_US |
dc.date.accessioned | 2012-08-08T08:48:45Z | - |
dc.date.available | 2012-08-08T08:48:45Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Blood, 1999, v. 93 n. 9, p. 3026-3032 | en_US |
dc.identifier.issn | 0006-4971 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/157298 | - |
dc.description.abstract | In addition to the Epstein-Barr virus (EBV) EBNA and LMP latency genes, there is a family of alternatively spliced BamHI-A rightward transcripts (BARTs). These latency transcripts are highly expressed in the EBV-associated malignancies nasopharyngeal carcinoma and Burkitt's lymphoma, and are expressed at lower levels in latently EBV-infected B-cell lines. The contribution of the BARTs to EBV biology or pathogenesis is unknown. Resting B cells have recently been recognized as a reservoir for EBV persistence in the peripheral blood. In these cells, EBV gene expression is tightly restricted and the only viral gene known to be consistently expressed is LMP2A. We used cell sorting and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine whether BARTs are expressed in the restricted form of in vivo latency. Our results demonstrated that RNAs with splicing diagnostic for transcripts containing the BART RPMS1 and BARFO open-reading frames (ORFs) were expressed in CD19+ but not in CD23+ B cells isolated from peripheral blood of healthy individuals. The product of the proximal RPMS1 ORF has not previously been characterized. The RPMS1 ORF was shown to encode a 15-kD protein that localized to the nucleus of transfected cells. Expression of the BARTs in peripheral blood B cells suggests that the proteins encoded by these transcripts are likely to be important for maintenance of in vivo latency. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/ | en_US |
dc.relation.ispartof | Blood | en_US |
dc.subject.mesh | Antigens, Cd - Analysis | en_US |
dc.subject.mesh | Antigens, Cd19 - Analysis | en_US |
dc.subject.mesh | B-Lymphocytes - Immunology - Virology | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Burkitt Lymphoma - Genetics | en_US |
dc.subject.mesh | Dna Primers | en_US |
dc.subject.mesh | Deoxyribonuclease Bamhi | en_US |
dc.subject.mesh | Epstein-Barr Virus Nuclear Antigens - Genetics | en_US |
dc.subject.mesh | Gene Expression Regulation, Viral | en_US |
dc.subject.mesh | Herpesvirus 4, Human - Genetics - Physiology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Open Reading Frames | en_US |
dc.subject.mesh | Restriction Mapping | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Sequence Alignment | en_US |
dc.subject.mesh | Sequence Homology, Nucleic Acid | en_US |
dc.subject.mesh | Transcription, Genetic | en_US |
dc.subject.mesh | Viral Matrix Proteins - Genetics | en_US |
dc.subject.mesh | Virus Latency - Genetics | en_US |
dc.title | Expression of Epstein-Barr virus BamHI-A rightward transcripts in latently infected B cells from peripheral blood | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, H:hlchen@hkucc.hku.hk | en_US |
dc.identifier.authority | Chen, H=rp00383 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1182/blood.V93.9.3026 | - |
dc.identifier.pmid | 10216099 | - |
dc.identifier.scopus | eid_2-s2.0-0033134768 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0033134768&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 93 | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.spage | 3026 | en_US |
dc.identifier.epage | 3032 | en_US |
dc.identifier.isi | WOS:000079984800032 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Chen, H=26643315400 | en_US |
dc.identifier.scopusauthorid | Smith, P=7406997818 | en_US |
dc.identifier.scopusauthorid | Ambinder, RF=7005598325 | en_US |
dc.identifier.scopusauthorid | Hayward, SD=7102776214 | en_US |
dc.identifier.issnl | 0006-4971 | - |