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- Publisher Website: 10.1093/jac/dkf208
- Scopus: eid_2-s2.0-0036849235
- PMID: 12407130
- WOS: WOS:000179034900015
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Article: Characterization of a laboratory-generated variant of BPS β-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime
Title | Characterization of a laboratory-generated variant of BPS β-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime |
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Authors | |
Issue Date | 2002 |
Publisher | Oxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/ |
Citation | Journal Of Antimicrobial Chemotherapy, 2002, v. 50 n. 5, p. 723-726 How to Cite? |
Abstract | Burkholderia pseudomallei produces an Ambler class A β-lactamase, known as BPS-1. The β-lactamase gene from a laboratory-derived, ceftazidime-resistant strain of B. pseudomallei (LH-1-2) was cloned and expressed in Escherichia coli. The β-lactamase, named BPS-1m, had an identical isoelectric focusing point (pl 7.7) to that of BPS-1, but differed in having a stronger hydrolytic activity against ceftazidime. Susceptibility testing showed that BPS-1m when expressed in E. coli conferred resistance to ceftazidime (MIC ≥ 32 mg/L). The amino acid sequence of BPS-1m differed from that of BPS-1 by a Pro-to-Ser change at position 167 in the omega loop. |
Persistent Identifier | http://hdl.handle.net/10722/157344 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 1.271 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ho, PL | en_US |
dc.contributor.author | Cheung, TKM | en_US |
dc.contributor.author | Yam, WC | en_US |
dc.contributor.author | Yuen, KY | en_US |
dc.date.accessioned | 2012-08-08T08:49:04Z | - |
dc.date.available | 2012-08-08T08:49:04Z | - |
dc.date.issued | 2002 | en_US |
dc.identifier.citation | Journal Of Antimicrobial Chemotherapy, 2002, v. 50 n. 5, p. 723-726 | en_US |
dc.identifier.issn | 0305-7453 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/157344 | - |
dc.description.abstract | Burkholderia pseudomallei produces an Ambler class A β-lactamase, known as BPS-1. The β-lactamase gene from a laboratory-derived, ceftazidime-resistant strain of B. pseudomallei (LH-1-2) was cloned and expressed in Escherichia coli. The β-lactamase, named BPS-1m, had an identical isoelectric focusing point (pl 7.7) to that of BPS-1, but differed in having a stronger hydrolytic activity against ceftazidime. Susceptibility testing showed that BPS-1m when expressed in E. coli conferred resistance to ceftazidime (MIC ≥ 32 mg/L). The amino acid sequence of BPS-1m differed from that of BPS-1 by a Pro-to-Ser change at position 167 in the omega loop. | en_US |
dc.language | eng | en_US |
dc.publisher | Oxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/ | en_US |
dc.relation.ispartof | Journal of Antimicrobial Chemotherapy | en_US |
dc.rights | Journal of Antimicrobial Chemotherapy. Copyright © Oxford University Press. | - |
dc.subject.mesh | Amino Acid Substitution - Genetics | en_US |
dc.subject.mesh | Burkholderia Pseudomallei - Enzymology - Genetics - Isolation & Purification | en_US |
dc.subject.mesh | Ceftazidime - Metabolism | en_US |
dc.subject.mesh | Genetic Variation | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Hydrolysis | en_US |
dc.subject.mesh | Beta-Lactam Resistance - Genetics | en_US |
dc.subject.mesh | Beta-Lactamases - Genetics - Metabolism | en_US |
dc.title | Characterization of a laboratory-generated variant of BPS β-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ho, PL:plho@hkucc.hku.hk | en_US |
dc.identifier.email | Yam, WC:wcyam@hkucc.hku.hk | en_US |
dc.identifier.email | Yuen, KY:kyyuen@hkucc.hku.hk | en_US |
dc.identifier.authority | Ho, PL=rp00406 | en_US |
dc.identifier.authority | Yam, WC=rp00313 | en_US |
dc.identifier.authority | Yuen, KY=rp00366 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1093/jac/dkf208 | - |
dc.identifier.pmid | 12407130 | - |
dc.identifier.scopus | eid_2-s2.0-0036849235 | en_US |
dc.identifier.hkuros | 83455 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036849235&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 50 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 723 | en_US |
dc.identifier.epage | 726 | en_US |
dc.identifier.isi | WOS:000179034900015 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Ho, PL=7402211363 | en_US |
dc.identifier.scopusauthorid | Cheung, TKM=7103334170 | en_US |
dc.identifier.scopusauthorid | Yam, WC=7004281720 | en_US |
dc.identifier.scopusauthorid | Yuen, KY=36078079100 | en_US |
dc.identifier.issnl | 0305-7453 | - |