File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1023/A:1004583910874
- Scopus: eid_2-s2.0-0344417886
- WOS: WOS:000081300600004
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Gradient and sensitivity enhanced multiple-quantum coherence in heteronuclear multidimensional NMR experiments
Title | Gradient and sensitivity enhanced multiple-quantum coherence in heteronuclear multidimensional NMR experiments |
---|---|
Authors | |
Keywords | Hmqc Multidimensional Nmr Protein Sensitivity Enhancement |
Issue Date | 1999 |
Publisher | Springer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0925-2738 |
Citation | Journal Of Biomolecular Nmr, 1999, v. 14 n. 2, p. 133-140 How to Cite? |
Abstract | Recent studies have indicated that the relaxation rate of the 1H- 13C multiple-quantum coherence is much slower than that of the 1H- 13C single- quantum coherence for non-aromatic methine sites in 13C labeled proteins and in nucleic acids at the slow tumbling limit. Several heteronuclear experiments have been designed to use a multiple-quantum coherence transfer scheme instead of the single-quantum transfer method, thereby increasing the sensitivity and resolution of the spectra. Here, we report a constant time, gradient and sensitivity enhanced HMQC experiment (CT-g/s-HMQC) and demonstrate that it has a significant sensitivity enhancement over constant time HMQC and constant time gradient and sensitivity enhanced HSQC experiments (CT-g/s-HSQC) when applied to a 13C and 15N labeled calmodulin sample in D 2O. We also apply this approach to 3D NOESY-HMQC and doubly sensitivity enhanced TOCSY-HMQC experiments, and demonstrate that they are more sensitive than their HSQC counterparts. |
Persistent Identifier | http://hdl.handle.net/10722/157376 |
ISSN | 2023 Impact Factor: 2.4 2023 SCImago Journal Rankings: 0.817 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kong, XM | en_US |
dc.contributor.author | Sze, KH | en_US |
dc.contributor.author | Zhu, G | en_US |
dc.date.accessioned | 2012-08-08T08:49:26Z | - |
dc.date.available | 2012-08-08T08:49:26Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Journal Of Biomolecular Nmr, 1999, v. 14 n. 2, p. 133-140 | en_US |
dc.identifier.issn | 0925-2738 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/157376 | - |
dc.description.abstract | Recent studies have indicated that the relaxation rate of the 1H- 13C multiple-quantum coherence is much slower than that of the 1H- 13C single- quantum coherence for non-aromatic methine sites in 13C labeled proteins and in nucleic acids at the slow tumbling limit. Several heteronuclear experiments have been designed to use a multiple-quantum coherence transfer scheme instead of the single-quantum transfer method, thereby increasing the sensitivity and resolution of the spectra. Here, we report a constant time, gradient and sensitivity enhanced HMQC experiment (CT-g/s-HMQC) and demonstrate that it has a significant sensitivity enhancement over constant time HMQC and constant time gradient and sensitivity enhanced HSQC experiments (CT-g/s-HSQC) when applied to a 13C and 15N labeled calmodulin sample in D 2O. We also apply this approach to 3D NOESY-HMQC and doubly sensitivity enhanced TOCSY-HMQC experiments, and demonstrate that they are more sensitive than their HSQC counterparts. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0925-2738 | en_US |
dc.relation.ispartof | Journal of Biomolecular NMR | en_US |
dc.subject | Hmqc | en_US |
dc.subject | Multidimensional Nmr | en_US |
dc.subject | Protein | en_US |
dc.subject | Sensitivity Enhancement | en_US |
dc.title | Gradient and sensitivity enhanced multiple-quantum coherence in heteronuclear multidimensional NMR experiments | en_US |
dc.type | Article | en_US |
dc.identifier.email | Sze, KH:khsze@hku.hk | en_US |
dc.identifier.authority | Sze, KH=rp00785 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1023/A:1004583910874 | en_US |
dc.identifier.scopus | eid_2-s2.0-0344417886 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0344417886&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 14 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 133 | en_US |
dc.identifier.epage | 140 | en_US |
dc.identifier.isi | WOS:000081300600004 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Kong, XM=7202794462 | en_US |
dc.identifier.scopusauthorid | Sze, KH=7006735061 | en_US |
dc.identifier.scopusauthorid | Zhu, G=7402633110 | en_US |
dc.identifier.issnl | 0925-2738 | - |