Titration of a SHIV1157ipd3N4 Stock by Intravenous Inoculation of Chinese-Origin Rhesus Macaques

Abstract

目的確定SHIV1157ipd3N4靜脈途徑感染中國恒河猴的有效病毒濃度,明確SHIV1157ipd3N4感染實驗猴體內病毒復制和免疫損傷情況。方法 10只正常中國恒河猴分成6組,分別用10倍系列稀釋的病毒液1 mL靜脈感染,測定血漿病毒載量,CD4+/CD8+,CD4+T淋巴細胞絕對數,分析感染后恒河猴體內病毒復制和免疫損傷情況。結果 5TCID50/mL以上濃度的SHIV1157ipd3N4能通過靜脈途徑感染中國恒河猴。結論該實驗的成功進行為SHIV/中國恒河猴疾病及評價模型的建立奠定了良好的基礎,為今后使用此模型評價抗病毒藥物或疫苗提供了條件。

Objective To determine the viral concentration of SHIV1157ipd3N4 to infect Chinese-origin rhesus macaques intravenously， and to clarify the viral replication and immunological impairment in the monkeys after SHIV1157ipd3N4 inoculation. Methods 10 Chinese-origin rhesus macaques were used in this study. Firstly, all the monkeys were screened by serological test to ensure that they were free of SIV,SRV, and STLV. Then they were infected with 1 mL of 10-fold serial dilution of SHIV1157ipd3N4 separately. Finally to make blood routine examination and measure the viral loads of the monkeys by real-time RT-PCR. Results More than 5 TCID50 /mL of SHIV1157ipd3N4 can infect the Chinese-origin rhesus macaques i. v. successfully. Conclusions Our findings of this study provide a firm basis for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus macaques intravenously. This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.