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- Publisher Website: 10.1007/s00775-007-0331-x
- Scopus: eid_2-s2.0-40949135318
- PMID: 18157556
- WOS: WOS:000253994300009
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Article: The effect of nitric oxide on metal release from metallothionein-3: Gradual unfolding of the protein
Title | The effect of nitric oxide on metal release from metallothionein-3: Gradual unfolding of the protein |
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Authors | |
Keywords | Growth inhibitory factor Metallothionein-3 Nitric oxide NMR Protein unfolding |
Issue Date | 2008 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00775/index.htm |
Citation | Journal Of Biological Inorganic Chemistry, 2008, v. 13 n. 3, p. 411-419 How to Cite? |
Abstract | Metallothionein-3 (MT-3), or neuronal growth inhibitory factor, which exhibits growth inhibitory activity, is a brain-specific metallothionein. In this study, the effect of nitric oxide (NO) on metal release (using Cd 2+ as a probe) from MT-3 was examined by 113Cd and 2D [1H-15N] heteronuclear single-quantum coherence NMR spectroscopy. The exposure of human MT-3 to NO leads to a nonselective release of the three metals from the β-domain. In contrast to metallothionein-1 and metallothionein-2, two of the bound metals in the α-domain were also partially released, with the domain structure remaining almost unchanged. Further addition of NO resulted in the complete release of metals and concomitant unfolding of the protein. The preference of release of the two metals in the α-domain was attributed to the presence of two slightly different coordination environments for the four cadmium/zinc atoms. © 2007 SBIC. |
Persistent Identifier | http://hdl.handle.net/10722/168285 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.543 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Wang, H | en_US |
dc.contributor.author | Li, H | en_US |
dc.contributor.author | Cai, B | en_US |
dc.contributor.author | Huang, ZX | en_US |
dc.contributor.author | Sun, H | en_US |
dc.date.accessioned | 2012-10-08T03:17:03Z | - |
dc.date.available | 2012-10-08T03:17:03Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Journal Of Biological Inorganic Chemistry, 2008, v. 13 n. 3, p. 411-419 | en_US |
dc.identifier.issn | 0949-8257 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/168285 | - |
dc.description.abstract | Metallothionein-3 (MT-3), or neuronal growth inhibitory factor, which exhibits growth inhibitory activity, is a brain-specific metallothionein. In this study, the effect of nitric oxide (NO) on metal release (using Cd 2+ as a probe) from MT-3 was examined by 113Cd and 2D [1H-15N] heteronuclear single-quantum coherence NMR spectroscopy. The exposure of human MT-3 to NO leads to a nonselective release of the three metals from the β-domain. In contrast to metallothionein-1 and metallothionein-2, two of the bound metals in the α-domain were also partially released, with the domain structure remaining almost unchanged. Further addition of NO resulted in the complete release of metals and concomitant unfolding of the protein. The preference of release of the two metals in the α-domain was attributed to the presence of two slightly different coordination environments for the four cadmium/zinc atoms. © 2007 SBIC. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00775/index.htm | en_US |
dc.relation.ispartof | Journal of Biological Inorganic Chemistry | en_US |
dc.subject | Growth inhibitory factor | - |
dc.subject | Metallothionein-3 | - |
dc.subject | Nitric oxide | - |
dc.subject | NMR | - |
dc.subject | Protein unfolding | - |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Metallothionein - Chemistry | en_US |
dc.subject.mesh | Metals - Chemistry | en_US |
dc.subject.mesh | Models, Molecular | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Nitric Oxide - Chemistry | en_US |
dc.subject.mesh | Nuclear Magnetic Resonance, Biomolecular | en_US |
dc.subject.mesh | Protein Denaturation | en_US |
dc.subject.mesh | Sequence Homology, Amino Acid | en_US |
dc.title | The effect of nitric oxide on metal release from metallothionein-3: Gradual unfolding of the protein | en_US |
dc.type | Article | en_US |
dc.identifier.email | Sun, H:hsun@hkucc.hku.hk | en_US |
dc.identifier.authority | Sun, H=rp00777 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/s00775-007-0331-x | en_US |
dc.identifier.pmid | 18157556 | - |
dc.identifier.scopus | eid_2-s2.0-40949135318 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-40949135318&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 13 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 411 | en_US |
dc.identifier.epage | 419 | en_US |
dc.identifier.isi | WOS:000253994300009 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Wang, H=15052706700 | en_US |
dc.identifier.scopusauthorid | Li, H=14023043100 | en_US |
dc.identifier.scopusauthorid | Cai, B=36484162900 | en_US |
dc.identifier.scopusauthorid | Huang, ZX=7406221847 | en_US |
dc.identifier.scopusauthorid | Sun, H=7404827446 | en_US |
dc.identifier.citeulike | 2752403 | - |
dc.identifier.issnl | 0949-8257 | - |