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Article: Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm
Title | Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm |
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Authors | |
Keywords | Cytokeratin Differentiation Ectoderm Endoderm Epithelial cell Gastrulation |
Issue Date | 2010 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/113463905 |
Citation | Anatomical Record, 2010, v. 293 n. 11, p. 1864-1872 How to Cite? |
Abstract | Although tridermic species have two junctional regions of ectoderm and endoderm between their epidermis and digestive tract, we actually know little about these particular boundaries. Cytokeratins are the major intermediate filaments of epithelial cells and show a high degree of tissue specificity. Therefore, to characterize the epithelial cells in the junctional region of ectoderm and endoderm, we immunohistochemically examined the localization of cytokeratins 5, 7/17, 14, 18, Sox17, and alpha-fetoprotein (AFP) in the oropharyngeal and anorectal regions during the mouse gastrulation process. At embryonic day (E) 9.5, cytokeratins 5, 7/17, 14, and 18 were detected in all epithelial cells of the oropharyngeal region. At E12.5, cytokeratin 5-positive cells were not observed in the middle area of the oral cavity; however, the immunoreactivity was strong in the anterior and posterior areas. The immunoreaction of cytokeratins 18 was seen only in the middle and posterior areas of the oral mucosa. Cytokeratins 7/17 and 14 were localized in all areas of the oropharyngeal region. Sox17 and AFP, which are endodermal markers, were detected in the middle and posterior areas of the oral mucosa, but not in the anterior area. Moreover, this same localization pattern of cytokeratins also existed in the anorectal region of the E12.5 embryo, suggesting that the localization of cytokeratins and endodermal markers might give an implication for the boundary between ectoderm and endoderm. These results also suggest that these cytokeratins are useful molecules for monitoring the epithelial cell differentiation in the junctional region of the germ layers. Anat Rec, 2010. © 2010 Wiley-Liss, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/169573 |
ISSN | 2023 Impact Factor: 1.8 2023 SCImago Journal Rankings: 0.615 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Hosoya, A | en_US |
dc.contributor.author | Kwak, S | en_US |
dc.contributor.author | Kim, EJ | en_US |
dc.contributor.author | Lunny, DP | en_US |
dc.contributor.author | Birgitte Lane, E | en_US |
dc.contributor.author | Cho, SW | en_US |
dc.contributor.author | Jung, HS | en_US |
dc.date.accessioned | 2012-10-25T04:53:01Z | - |
dc.date.available | 2012-10-25T04:53:01Z | - |
dc.date.issued | 2010 | en_US |
dc.identifier.citation | Anatomical Record, 2010, v. 293 n. 11, p. 1864-1872 | en_US |
dc.identifier.issn | 1932-8486 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/169573 | - |
dc.description.abstract | Although tridermic species have two junctional regions of ectoderm and endoderm between their epidermis and digestive tract, we actually know little about these particular boundaries. Cytokeratins are the major intermediate filaments of epithelial cells and show a high degree of tissue specificity. Therefore, to characterize the epithelial cells in the junctional region of ectoderm and endoderm, we immunohistochemically examined the localization of cytokeratins 5, 7/17, 14, 18, Sox17, and alpha-fetoprotein (AFP) in the oropharyngeal and anorectal regions during the mouse gastrulation process. At embryonic day (E) 9.5, cytokeratins 5, 7/17, 14, and 18 were detected in all epithelial cells of the oropharyngeal region. At E12.5, cytokeratin 5-positive cells were not observed in the middle area of the oral cavity; however, the immunoreactivity was strong in the anterior and posterior areas. The immunoreaction of cytokeratins 18 was seen only in the middle and posterior areas of the oral mucosa. Cytokeratins 7/17 and 14 were localized in all areas of the oropharyngeal region. Sox17 and AFP, which are endodermal markers, were detected in the middle and posterior areas of the oral mucosa, but not in the anterior area. Moreover, this same localization pattern of cytokeratins also existed in the anorectal region of the E12.5 embryo, suggesting that the localization of cytokeratins and endodermal markers might give an implication for the boundary between ectoderm and endoderm. These results also suggest that these cytokeratins are useful molecules for monitoring the epithelial cell differentiation in the junctional region of the germ layers. Anat Rec, 2010. © 2010 Wiley-Liss, Inc. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/113463905 | en_US |
dc.relation.ispartof | Anatomical Record | en_US |
dc.subject | Cytokeratin | - |
dc.subject | Differentiation | - |
dc.subject | Ectoderm | - |
dc.subject | Endoderm | - |
dc.subject | Epithelial cell | - |
dc.subject | Gastrulation | - |
dc.subject.mesh | Anal Canal - Cytology - Metabolism | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Biological Markers | en_US |
dc.subject.mesh | Cell Differentiation | en_US |
dc.subject.mesh | Ectoderm - Cytology - Metabolism | en_US |
dc.subject.mesh | Embryo, Mammalian - Cytology - Metabolism | en_US |
dc.subject.mesh | Endoderm - Cytology - Metabolism | en_US |
dc.subject.mesh | Epithelial Cells - Cytology - Metabolism | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Gastrulation | en_US |
dc.subject.mesh | Intercellular Junctions - Metabolism | en_US |
dc.subject.mesh | Keratin-14 - Metabolism | en_US |
dc.subject.mesh | Keratin-18 - Metabolism | en_US |
dc.subject.mesh | Keratin-5 - Metabolism | en_US |
dc.subject.mesh | Keratin-7 - Metabolism | en_US |
dc.subject.mesh | Keratins - Metabolism | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Oropharynx - Cytology - Metabolism | en_US |
dc.subject.mesh | Pregnancy | en_US |
dc.subject.mesh | Rectum - Cytology - Metabolism | en_US |
dc.title | Immunohistochemical Localization of Cytokeratins in the Junctional Region of Ectoderm and Endoderm | en_US |
dc.type | Article | en_US |
dc.identifier.email | Jung, HS: hsjung@yuhs.ac | en_US |
dc.identifier.authority | Jung, HS=rp01683 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/ar.21233 | en_US |
dc.identifier.pmid | 20818615 | - |
dc.identifier.scopus | eid_2-s2.0-78049482943 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-78049482943&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 293 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.spage | 1864 | en_US |
dc.identifier.epage | 1872 | en_US |
dc.identifier.isi | WOS:000283958300007 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Hosoya, A=8651007100 | en_US |
dc.identifier.scopusauthorid | Kwak, S=7202607781 | en_US |
dc.identifier.scopusauthorid | Kim, EJ=36064163200 | en_US |
dc.identifier.scopusauthorid | Lunny, DP=6603385849 | en_US |
dc.identifier.scopusauthorid | Birgitte Lane, E=24319822100 | en_US |
dc.identifier.scopusauthorid | Cho, SW=32967447200 | en_US |
dc.identifier.scopusauthorid | Jung, HS=7403030195 | en_US |
dc.identifier.issnl | 1932-8486 | - |