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- Publisher Website: 10.1161/01.CIR.80.3.643
- Scopus: eid_2-s2.0-0024447397
- PMID: 2766515
- WOS: WOS:A1989AP70600025
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Article: Endothelium-dependent inhibition of ergonovine-induced contraction is impaired in porcine coronary arteries with regenerated endothelium
Title | Endothelium-dependent inhibition of ergonovine-induced contraction is impaired in porcine coronary arteries with regenerated endothelium |
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Authors | |
Issue Date | 1989 |
Publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.org |
Citation | Circulation, 1989, v. 80 n. 3, p. 643-650 How to Cite? |
Abstract | The inhibitory effects of the endothelium against ergonovine-induced contraction were examined in isolated porcine coronary arteries under normal conditions and after endothelial regeneration. Endothelium-dependent responses were examined in vitro in normal Yorkshire pigs (n = 16) and in pigs that had undergone balloon endothelium removal of the left anterior descending coronary artery (LAD) 4 weeks before the study (n = 10). The presence of a complete endothelial lining was confirmed histologically. In rings from normal arteries contracted with prostaglandin F(2α) in the presence of indomethacin and ketanserin (a 5-HT2-serotonergic blocker), ergonovine caused endothelium-dependent relaxations. They were attenuated by rauwolscine (an α2-adrenergic blocker), inhibited by methiothepin (a combined 5-HT1- and 5-HT2-serotonergic blocker) or by pertussis toxin (an inhibitor of several G proteins) and abolished by oxyhemoglobin (a selective inactivator of endothelium-derived relaxing factor). In quiescent rings from normal arteries, ergonovine caused contractions that were inhibited by the presence of the endothelium; this endothelium-dependent inhibition was ablished by oxyhemoglobin. The direct contractions were not affected by prazosin (an α1-adrenergic blocker), rauwolscine, 6-hydroxydopamine (an agent causing chemical sympathectomy), or diphenhydramine (an H1-histaminergic blocker) but were inhibited by ketanserin. In rings with regenerated endothelium contracted with prostaglandin F(2α), the endothelium-dependent relaxations to ergonovine were reduced significantly and were not inhibited by pertussis toxin. In quiescent rings with regenerated endothelium, the endothelium-dependent inhibition of ergonovine-induced contraction was less. Oxyhemoglobin caused endothelium-dependent contractions in quiescent rings (an indirect index of basally released endothelium-derived relaxing factor) that were reduced significantly in quiescent rings with regenerated endothelium. These results indicate that 1) the endothelium exerts its inhibitory action against ergonovine-induced contractioins by the release of endothelium-derived relaxing factor under basal conditions and upon stimulation by ergonovine, 2) endothelium-dependent relaxations to ergonovine are mediated mainly by 5-HT1-serotonergic receptors, whereas the direct contractions are mediated by 5-HT2-serotonergic receptors with little contribution of α-adrenoceptors, 3) the inhibitory role of the endothelium is impaired significantly in the regenerated state because of the reduced ability to release the relaxing factor, and 4) endothelial pertussis toxin-sensitive G protein may be involved in the synthesis of the relaxing factor upon stimulation by ergonovine, and dysfunction of the G protein may account partly for the dysfunction of regenerated endothelium. |
Persistent Identifier | http://hdl.handle.net/10722/170940 |
ISSN | 2023 Impact Factor: 35.5 2023 SCImago Journal Rankings: 8.415 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Shimokawa, H | en_US |
dc.contributor.author | Flavahan, NA | en_US |
dc.contributor.author | Shepherd, JT | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:11:31Z | - |
dc.date.available | 2012-10-30T06:11:31Z | - |
dc.date.issued | 1989 | en_US |
dc.identifier.citation | Circulation, 1989, v. 80 n. 3, p. 643-650 | en_US |
dc.identifier.issn | 0009-7322 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/170940 | - |
dc.description.abstract | The inhibitory effects of the endothelium against ergonovine-induced contraction were examined in isolated porcine coronary arteries under normal conditions and after endothelial regeneration. Endothelium-dependent responses were examined in vitro in normal Yorkshire pigs (n = 16) and in pigs that had undergone balloon endothelium removal of the left anterior descending coronary artery (LAD) 4 weeks before the study (n = 10). The presence of a complete endothelial lining was confirmed histologically. In rings from normal arteries contracted with prostaglandin F(2α) in the presence of indomethacin and ketanserin (a 5-HT2-serotonergic blocker), ergonovine caused endothelium-dependent relaxations. They were attenuated by rauwolscine (an α2-adrenergic blocker), inhibited by methiothepin (a combined 5-HT1- and 5-HT2-serotonergic blocker) or by pertussis toxin (an inhibitor of several G proteins) and abolished by oxyhemoglobin (a selective inactivator of endothelium-derived relaxing factor). In quiescent rings from normal arteries, ergonovine caused contractions that were inhibited by the presence of the endothelium; this endothelium-dependent inhibition was ablished by oxyhemoglobin. The direct contractions were not affected by prazosin (an α1-adrenergic blocker), rauwolscine, 6-hydroxydopamine (an agent causing chemical sympathectomy), or diphenhydramine (an H1-histaminergic blocker) but were inhibited by ketanserin. In rings with regenerated endothelium contracted with prostaglandin F(2α), the endothelium-dependent relaxations to ergonovine were reduced significantly and were not inhibited by pertussis toxin. In quiescent rings with regenerated endothelium, the endothelium-dependent inhibition of ergonovine-induced contraction was less. Oxyhemoglobin caused endothelium-dependent contractions in quiescent rings (an indirect index of basally released endothelium-derived relaxing factor) that were reduced significantly in quiescent rings with regenerated endothelium. These results indicate that 1) the endothelium exerts its inhibitory action against ergonovine-induced contractioins by the release of endothelium-derived relaxing factor under basal conditions and upon stimulation by ergonovine, 2) endothelium-dependent relaxations to ergonovine are mediated mainly by 5-HT1-serotonergic receptors, whereas the direct contractions are mediated by 5-HT2-serotonergic receptors with little contribution of α-adrenoceptors, 3) the inhibitory role of the endothelium is impaired significantly in the regenerated state because of the reduced ability to release the relaxing factor, and 4) endothelial pertussis toxin-sensitive G protein may be involved in the synthesis of the relaxing factor upon stimulation by ergonovine, and dysfunction of the G protein may account partly for the dysfunction of regenerated endothelium. | en_US |
dc.language | eng | en_US |
dc.publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://circ.ahajournals.org | en_US |
dc.relation.ispartof | Circulation | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Coronary Vessels - Drug Effects - Physiology | en_US |
dc.subject.mesh | Dose-Response Relationship, Drug | en_US |
dc.subject.mesh | Drug Interactions | en_US |
dc.subject.mesh | Endothelium, Vascular - Drug Effects - Physiology | en_US |
dc.subject.mesh | Ergonovine - Pharmacology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Myocardial Contraction - Drug Effects | en_US |
dc.subject.mesh | Organ Culture Techniques | en_US |
dc.subject.mesh | Regeneration - Drug Effects | en_US |
dc.subject.mesh | Swine | en_US |
dc.title | Endothelium-dependent inhibition of ergonovine-induced contraction is impaired in porcine coronary arteries with regenerated endothelium | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1161/01.CIR.80.3.643 | - |
dc.identifier.pmid | 2766515 | - |
dc.identifier.scopus | eid_2-s2.0-0024447397 | en_US |
dc.identifier.volume | 80 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 643 | en_US |
dc.identifier.epage | 650 | en_US |
dc.identifier.isi | WOS:A1989AP70600025 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Shimokawa, H=16684837100 | en_US |
dc.identifier.scopusauthorid | Flavahan, NA=7006398882 | en_US |
dc.identifier.scopusauthorid | Shepherd, JT=7401742522 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0009-7322 | - |