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Article: Isoproterenol causes hyperpolarization through opening of ATP-sensitive potassium channels in vascular smooth muscle of the canine saphenous vein
Title | Isoproterenol causes hyperpolarization through opening of ATP-sensitive potassium channels in vascular smooth muscle of the canine saphenous vein |
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Authors | |
Issue Date | 1995 |
Publisher | American Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org |
Citation | Journal Of Pharmacology And Experimental Therapeutics, 1995, v. 272 n. 1, p. 379-384 How to Cite? |
Abstract | Experiments were designed to determine how isoproterenol affects the cell membrane potential of smooth muscle cells of the canine saphenous vein. Measurements of membrane potential were performed using glass microelectrodes. Isoproterenol (10-9 to 10-6 M) caused sustained, concentration-dependent membrane hyperpolarizations in tissues with and without endothelium. ICI 118,551 (a selective β2-adrenoceptor antagonist), but not atenolol (a selective β1-adrenoceptor antagonist), abolished the hyperpolarization to isoproterenol. Forskolin, an activator of adenylate cyclase, produced sustained hyperpolarizations, which were not mimicked by 1,9-dideoxyforskolin. Incubation with either ouabain or extracellular K+- free solution did not inhibit the electrical response to isoproterenol. Glibenclamide (a selective ATP-sensitive K+ channel antagonist) attenuated the hyperpolarizations induced by either isoproterenol or forskolin, whereas charybdotoxin (an inhibitor of large conductance Ca++-activated K+ channels) did not. These findings suggest that isoproterenol opens ATP- sensitive K+ channels indirectly through activation of adenylate cyclase in the smooth muscle of the canine saphenous vein. The adrenergic receptor involved belongs to the β2-adrenoceptor subtype. |
Persistent Identifier | http://hdl.handle.net/10722/171156 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.829 |
DC Field | Value | Language |
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dc.contributor.author | Nakashima, M | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-10-30T06:12:26Z | - |
dc.date.available | 2012-10-30T06:12:26Z | - |
dc.date.issued | 1995 | en_US |
dc.identifier.citation | Journal Of Pharmacology And Experimental Therapeutics, 1995, v. 272 n. 1, p. 379-384 | en_US |
dc.identifier.issn | 0022-3565 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171156 | - |
dc.description.abstract | Experiments were designed to determine how isoproterenol affects the cell membrane potential of smooth muscle cells of the canine saphenous vein. Measurements of membrane potential were performed using glass microelectrodes. Isoproterenol (10-9 to 10-6 M) caused sustained, concentration-dependent membrane hyperpolarizations in tissues with and without endothelium. ICI 118,551 (a selective β2-adrenoceptor antagonist), but not atenolol (a selective β1-adrenoceptor antagonist), abolished the hyperpolarization to isoproterenol. Forskolin, an activator of adenylate cyclase, produced sustained hyperpolarizations, which were not mimicked by 1,9-dideoxyforskolin. Incubation with either ouabain or extracellular K+- free solution did not inhibit the electrical response to isoproterenol. Glibenclamide (a selective ATP-sensitive K+ channel antagonist) attenuated the hyperpolarizations induced by either isoproterenol or forskolin, whereas charybdotoxin (an inhibitor of large conductance Ca++-activated K+ channels) did not. These findings suggest that isoproterenol opens ATP- sensitive K+ channels indirectly through activation of adenylate cyclase in the smooth muscle of the canine saphenous vein. The adrenergic receptor involved belongs to the β2-adrenoceptor subtype. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org | en_US |
dc.relation.ispartof | Journal of Pharmacology and Experimental Therapeutics | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Atenolol - Pharmacology | en_US |
dc.subject.mesh | Charybdotoxin | en_US |
dc.subject.mesh | Dogs | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Forskolin - Pharmacology | en_US |
dc.subject.mesh | Glyburide - Pharmacology | en_US |
dc.subject.mesh | Ion Channel Gating - Drug Effects | en_US |
dc.subject.mesh | Isoproterenol - Pharmacology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Potentials - Drug Effects | en_US |
dc.subject.mesh | Muscle, Smooth, Vascular - Drug Effects | en_US |
dc.subject.mesh | Ouabain - Pharmacology | en_US |
dc.subject.mesh | Potassium Channels - Drug Effects | en_US |
dc.subject.mesh | Propanolamines - Pharmacology | en_US |
dc.subject.mesh | Receptors, Adrenergic, Beta - Drug Effects | en_US |
dc.subject.mesh | Saphenous Vein | en_US |
dc.subject.mesh | Scorpion Venoms - Pharmacology | en_US |
dc.subject.mesh | Yohimbine - Pharmacology | en_US |
dc.title | Isoproterenol causes hyperpolarization through opening of ATP-sensitive potassium channels in vascular smooth muscle of the canine saphenous vein | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, PM:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 7529310 | - |
dc.identifier.scopus | eid_2-s2.0-0028893949 | en_US |
dc.identifier.volume | 272 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 379 | en_US |
dc.identifier.epage | 384 | en_US |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Nakashima, M=35599797500 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, PM=7202304247 | en_US |
dc.identifier.issnl | 0022-3565 | - |