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- Publisher Website: 10.1095/biolreprod64.3.764
- Scopus: eid_2-s2.0-0035113314
- PMID: 11207189
- WOS: WOS:000167120400004
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Article: Na+ reabsorption in cultured rat epididymal epithelium via the Na+/nucleoside cotransporter
Title | Na+ reabsorption in cultured rat epididymal epithelium via the Na+/nucleoside cotransporter |
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Authors | |
Keywords | Epididymis |
Issue Date | 2001 |
Publisher | Society for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/ |
Citation | Biology Of Reproduction, 2001, v. 64 n. 3, p. 764-769 How to Cite? |
Abstract | The effect of nucleoside on Na+ reabsorption via Na+/nucleoside cotransporter in cultured rat epididymal epithelia was studied by short-circuit current (Isc) technique. Guanosine added apically stimulated Isc in a dose-dependent manner, with a median effective concentration (EC50) of 7 ± 2 μM (mean ± SEM). Removal of Na+ from the apical bathing solution or pretreatment with a nonspecific Na+/nucleoside cotransporter inhibitor, phloridzin, completely blocked the Isc response to guanosine. Moreover, the guanosine response was abolished by pretreatment of the tissue with ouabain, a Na+/K+-ATPase inhibitor, suggesting the involvement of Na+/nucleoside cotransporter on the apical side and Na+/K+-ATPase on the basolateral side in Na+ reabsorption. In contrast, the Isc response to guanosine was not affected after desensitization of purinoceptors by ATP. Addition of the Na+/K+/2Cl- symport inhibitor bumetanide to the basolateral side or the nonspecific Cl- channel blocker diphenylamine-2-carboxylate to the apical side showed no effect on the Isc response to guanosine, excluding stimulation of Cl- secretion by guanosine as the cause of the guanosine-induced Isc. The Isc response to purine nucleoside (guanosine and inosine) was much higher than that to pyrimidine nucleoside (thymidine and cytidine). Consistent with substrate specificity, results of reverse transcription-polymerase chain reaction revealed mRNA for concentrative nucleoside transporter (CNT2), which is a purine nucleoside-selective Na+/nucleoside cotransporter in the epididymis, but not for CNT1. It is suggested that the Na+/nucleoside cotransporter (i.e., CNT2) may be one of the elements involved in Na+ and fluid reabsorption in the epididymis, thereby providing an optimal microenvironment for the maturation and storage of spermatozoa. |
Persistent Identifier | http://hdl.handle.net/10722/171267 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 1.022 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Leung, GPH | en_US |
dc.contributor.author | Cheung, KH | en_US |
dc.contributor.author | Tse, CM | en_US |
dc.contributor.author | Wong, PYD | en_US |
dc.date.accessioned | 2012-10-30T06:13:03Z | - |
dc.date.available | 2012-10-30T06:13:03Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | Biology Of Reproduction, 2001, v. 64 n. 3, p. 764-769 | en_US |
dc.identifier.issn | 0006-3363 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171267 | - |
dc.description.abstract | The effect of nucleoside on Na+ reabsorption via Na+/nucleoside cotransporter in cultured rat epididymal epithelia was studied by short-circuit current (Isc) technique. Guanosine added apically stimulated Isc in a dose-dependent manner, with a median effective concentration (EC50) of 7 ± 2 μM (mean ± SEM). Removal of Na+ from the apical bathing solution or pretreatment with a nonspecific Na+/nucleoside cotransporter inhibitor, phloridzin, completely blocked the Isc response to guanosine. Moreover, the guanosine response was abolished by pretreatment of the tissue with ouabain, a Na+/K+-ATPase inhibitor, suggesting the involvement of Na+/nucleoside cotransporter on the apical side and Na+/K+-ATPase on the basolateral side in Na+ reabsorption. In contrast, the Isc response to guanosine was not affected after desensitization of purinoceptors by ATP. Addition of the Na+/K+/2Cl- symport inhibitor bumetanide to the basolateral side or the nonspecific Cl- channel blocker diphenylamine-2-carboxylate to the apical side showed no effect on the Isc response to guanosine, excluding stimulation of Cl- secretion by guanosine as the cause of the guanosine-induced Isc. The Isc response to purine nucleoside (guanosine and inosine) was much higher than that to pyrimidine nucleoside (thymidine and cytidine). Consistent with substrate specificity, results of reverse transcription-polymerase chain reaction revealed mRNA for concentrative nucleoside transporter (CNT2), which is a purine nucleoside-selective Na+/nucleoside cotransporter in the epididymis, but not for CNT1. It is suggested that the Na+/nucleoside cotransporter (i.e., CNT2) may be one of the elements involved in Na+ and fluid reabsorption in the epididymis, thereby providing an optimal microenvironment for the maturation and storage of spermatozoa. | en_US |
dc.language | eng | en_US |
dc.publisher | Society for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/ | en_US |
dc.relation.ispartof | Biology of Reproduction | en_US |
dc.subject | Epididymis | - |
dc.subject.mesh | Absorption | en_US |
dc.subject.mesh | Adenosine Triphosphate - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Anthranilic Acids - Pharmacology | en_US |
dc.subject.mesh | Bumetanide - Pharmacology | en_US |
dc.subject.mesh | Calcium Channel Blockers - Pharmacology | en_US |
dc.subject.mesh | Carrier Proteins - Biosynthesis - Genetics - Metabolism | en_US |
dc.subject.mesh | Chloride Channels - Antagonists & Inhibitors - Physiology | en_US |
dc.subject.mesh | Dna - Chemistry - Genetics - Metabolism | en_US |
dc.subject.mesh | Epididymis - Metabolism | en_US |
dc.subject.mesh | Epithelial Cells - Metabolism | en_US |
dc.subject.mesh | Gene Expression | en_US |
dc.subject.mesh | Guanosine - Pharmacology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Membrane Transport Proteins | en_US |
dc.subject.mesh | Patch-Clamp Techniques | en_US |
dc.subject.mesh | Phlorhizin - Pharmacology | en_US |
dc.subject.mesh | Rna, Messenger - Genetics - Metabolism | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Sodium - Metabolism | en_US |
dc.subject.mesh | Sodium-Potassium-Exchanging Atpase - Antagonists & Inhibitors - Metabolism | en_US |
dc.subject.mesh | Substrate Specificity | en_US |
dc.title | Na+ reabsorption in cultured rat epididymal epithelium via the Na+/nucleoside cotransporter | en_US |
dc.type | Article | en_US |
dc.identifier.email | Leung, GPH:gphleung@hkucc.hku.hk | en_US |
dc.identifier.email | Cheung, KH:kingho.cheung@hku.hk | en_US |
dc.identifier.authority | Leung, GPH=rp00234 | en_US |
dc.identifier.authority | Cheung, KH=rp01463 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1095/biolreprod64.3.764 | - |
dc.identifier.pmid | 11207189 | - |
dc.identifier.scopus | eid_2-s2.0-0035113314 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0035113314&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 64 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 764 | en_US |
dc.identifier.epage | 769 | en_US |
dc.identifier.isi | WOS:000167120400004 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Leung, GPH=35963668200 | en_US |
dc.identifier.scopusauthorid | Cheung, KH=14007487800 | en_US |
dc.identifier.scopusauthorid | Tse, CM=7103295076 | en_US |
dc.identifier.scopusauthorid | Wong, PYD=7403980262 | en_US |
dc.identifier.issnl | 0006-3363 | - |