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- Publisher Website: 10.1016/0024-3205(92)90427-Q
- Scopus: eid_2-s2.0-0026611419
- PMID: 1316983
- WOS: WOS:A1992HQ39700008
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Article: 2[125I]Iodomelatonin binding sites in spleens of guinea pigs
Title | 2[125I]Iodomelatonin binding sites in spleens of guinea pigs |
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Authors | |
Issue Date | 1992 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie |
Citation | Life Sciences, 1992, v. 50 n. 22, p. 1719-1726 How to Cite? |
Abstract | 2-[125I]Iodomelatonin was found to bind specifically to the membrane preparations of the spleens of guinea pigs with high affinity. The binding was rapid, stable, saturable and reversible. Scatchard analysis of the binding assays revealed an equilibrium dissociation constant (Kd) of 49.8 ± 4.12 pmol/l and binding site density (Bmax) of 0.69 ± 0.082 fmol/mg protein at mid-light (n = 10). There was no significant change in the Kd (41.8 ± 3.16 pmol/l) or the Bmax (0.58 ± 0.070 fmol/mg protein) at mid-dark (n = 10). Kinetic analysis showed a Kd of 23.13 ± 4.81 pmol/l (mean ± SE, n = 4), in agreement to that derived from the saturation studies. The 2-[125I]iodomelatonin binding sites have the following order of potency: 2-iodomelatonin > melatonin > 6-chloromelatonin >> N-acetylserotonin, 6-hydroxymelatonin > 5-methoxytryptamine, 5 methoxytryptophol > serotonin, 5-methoxyindole-3-acetic acid > 5-hydroxytryptophol, 3-acetylindole, 1-acetylindole-3-carboxyaldehyde, L-tryptophan > tryptamine, 5-hydroxyindole-3-acetic acid. Differential centrifugation studies showed that the binding sites are localized mainly in the nuclear fraction (65.5%), the rest are distributed in the microsomal fraction (17.4%), mitochondrial fraction (14.7%) and cytosolic fraction (0.3%). The demonstration of 2-[125I]iodomelatonin binding sites in the spleen suggests the presence of melatonin receptors and a direct mechanism of action of melatonin on the immune system. |
Persistent Identifier | http://hdl.handle.net/10722/171568 |
ISSN | 2023 Impact Factor: 5.2 2023 SCImago Journal Rankings: 1.257 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Poon, AMS | en_US |
dc.contributor.author | Pang, SF | en_US |
dc.date.accessioned | 2012-10-30T06:15:43Z | - |
dc.date.available | 2012-10-30T06:15:43Z | - |
dc.date.issued | 1992 | en_US |
dc.identifier.citation | Life Sciences, 1992, v. 50 n. 22, p. 1719-1726 | en_US |
dc.identifier.issn | 0024-3205 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171568 | - |
dc.description.abstract | 2-[125I]Iodomelatonin was found to bind specifically to the membrane preparations of the spleens of guinea pigs with high affinity. The binding was rapid, stable, saturable and reversible. Scatchard analysis of the binding assays revealed an equilibrium dissociation constant (Kd) of 49.8 ± 4.12 pmol/l and binding site density (Bmax) of 0.69 ± 0.082 fmol/mg protein at mid-light (n = 10). There was no significant change in the Kd (41.8 ± 3.16 pmol/l) or the Bmax (0.58 ± 0.070 fmol/mg protein) at mid-dark (n = 10). Kinetic analysis showed a Kd of 23.13 ± 4.81 pmol/l (mean ± SE, n = 4), in agreement to that derived from the saturation studies. The 2-[125I]iodomelatonin binding sites have the following order of potency: 2-iodomelatonin > melatonin > 6-chloromelatonin >> N-acetylserotonin, 6-hydroxymelatonin > 5-methoxytryptamine, 5 methoxytryptophol > serotonin, 5-methoxyindole-3-acetic acid > 5-hydroxytryptophol, 3-acetylindole, 1-acetylindole-3-carboxyaldehyde, L-tryptophan > tryptamine, 5-hydroxyindole-3-acetic acid. Differential centrifugation studies showed that the binding sites are localized mainly in the nuclear fraction (65.5%), the rest are distributed in the microsomal fraction (17.4%), mitochondrial fraction (14.7%) and cytosolic fraction (0.3%). The demonstration of 2-[125I]iodomelatonin binding sites in the spleen suggests the presence of melatonin receptors and a direct mechanism of action of melatonin on the immune system. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie | en_US |
dc.relation.ispartof | Life Sciences | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Binding Sites | en_US |
dc.subject.mesh | Cell Nucleus - Metabolism | en_US |
dc.subject.mesh | Circadian Rhythm | en_US |
dc.subject.mesh | Cytosol - Metabolism | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Guinea Pigs | en_US |
dc.subject.mesh | Iodine Radioisotopes | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Melatonin - Analogs & Derivatives - Metabolism | en_US |
dc.subject.mesh | Microsomes - Metabolism | en_US |
dc.subject.mesh | Mitochondria - Metabolism | en_US |
dc.subject.mesh | Receptors, Melatonin | en_US |
dc.subject.mesh | Receptors, Neurotransmitter - Metabolism | en_US |
dc.subject.mesh | Spleen - Metabolism | en_US |
dc.title | 2[125I]Iodomelatonin binding sites in spleens of guinea pigs | en_US |
dc.type | Article | en_US |
dc.identifier.email | Poon, AMS:amspoon@hkucc.hku.hk | en_US |
dc.identifier.authority | Poon, AMS=rp00354 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0024-3205(92)90427-Q | en_US |
dc.identifier.pmid | 1316983 | - |
dc.identifier.scopus | eid_2-s2.0-0026611419 | en_US |
dc.identifier.volume | 50 | en_US |
dc.identifier.issue | 22 | en_US |
dc.identifier.spage | 1719 | en_US |
dc.identifier.epage | 1726 | en_US |
dc.identifier.isi | WOS:A1992HQ39700008 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Poon, AMS=7103068868 | en_US |
dc.identifier.scopusauthorid | Pang, SF=7402528719 | en_US |
dc.identifier.issnl | 0024-3205 | - |