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- PMID: 1307932
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Article: Nonendothelial aortic source of nitric oxide in Wistar-Kyoto normotensive and spontaneous hypertensive rats.
Title | Nonendothelial aortic source of nitric oxide in Wistar-Kyoto normotensive and spontaneous hypertensive rats. |
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Authors | |
Issue Date | 1992 |
Publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/NSG |
Citation | Biological Signals, 1992, v. 1 n. 6, p. 322-330 How to Cite? |
Abstract | Using a recognized inhibitor of nitric oxide (NO) synthesis, Nw-nitro L-arginine methyl ester (L-NAME), we tested the hypothesis of the existence of a nonendothelial source of NO in vascular tissue using rings of rat thoracic aorta in which endothelial cells have been removed by mechanical abrasion and have totally lost their endothelium-dependent relaxation. Contractility of the muscle was tested by recording the concentration-dependent contraction of the preparations induced by an alpha-adrenergic agonist, phenylephrine. Contractility in aortas from Wistar-Kyoto normotensive rats (WKY) and spontaneous hypertensive rats (SHR) was not significantly affected by a 30-min to 2-hour incubation with L-NAME prior to agonist stimulation. However, preparations incubated for 30 min with 1 mM L-arginine (L-ARG) and then washed for 1 h in standard Krebs solution had a significantly reduced contraction to phenylephrine in both WKY and SHR. In these preparations pretreated with L-ARG, L-NAME significantly increased contractility in both WKY and SHR; this effect was prevented by L-ARG but not by D-arginine. Responses to phenylephrine were not inhibited by L-ARG when preparations were incubated from the beginning of the experiment with 1 mM cycloheximide, thus suggesting a dependence on protein synthesis of the attenuation of contraction seen with L-ARG. Intact aortic rings processed for NADPH diaphorase histochemistry, a putative marker for NO synthase, showed NADPH diaphorase reactivity only in the endothelial layer and in the adventitia.(ABSTRACT TRUNCATED AT 250 WORDS) |
Persistent Identifier | http://hdl.handle.net/10722/171571 |
ISSN |
DC Field | Value | Language |
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dc.contributor.author | Bourreau, JP | en_US |
dc.contributor.author | Kitchener, P | en_US |
dc.contributor.author | Kwan, CY | en_US |
dc.contributor.author | Daniel, EE | en_US |
dc.date.accessioned | 2012-10-30T06:15:44Z | - |
dc.date.available | 2012-10-30T06:15:44Z | - |
dc.date.issued | 1992 | en_US |
dc.identifier.citation | Biological Signals, 1992, v. 1 n. 6, p. 322-330 | en_US |
dc.identifier.issn | 1016-0922 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171571 | - |
dc.description.abstract | Using a recognized inhibitor of nitric oxide (NO) synthesis, Nw-nitro L-arginine methyl ester (L-NAME), we tested the hypothesis of the existence of a nonendothelial source of NO in vascular tissue using rings of rat thoracic aorta in which endothelial cells have been removed by mechanical abrasion and have totally lost their endothelium-dependent relaxation. Contractility of the muscle was tested by recording the concentration-dependent contraction of the preparations induced by an alpha-adrenergic agonist, phenylephrine. Contractility in aortas from Wistar-Kyoto normotensive rats (WKY) and spontaneous hypertensive rats (SHR) was not significantly affected by a 30-min to 2-hour incubation with L-NAME prior to agonist stimulation. However, preparations incubated for 30 min with 1 mM L-arginine (L-ARG) and then washed for 1 h in standard Krebs solution had a significantly reduced contraction to phenylephrine in both WKY and SHR. In these preparations pretreated with L-ARG, L-NAME significantly increased contractility in both WKY and SHR; this effect was prevented by L-ARG but not by D-arginine. Responses to phenylephrine were not inhibited by L-ARG when preparations were incubated from the beginning of the experiment with 1 mM cycloheximide, thus suggesting a dependence on protein synthesis of the attenuation of contraction seen with L-ARG. Intact aortic rings processed for NADPH diaphorase histochemistry, a putative marker for NO synthase, showed NADPH diaphorase reactivity only in the endothelial layer and in the adventitia.(ABSTRACT TRUNCATED AT 250 WORDS) | en_US |
dc.language | eng | en_US |
dc.publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/NSG | en_US |
dc.relation.ispartof | Biological signals | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Aorta, Thoracic - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Arginine - Analogs & Derivatives - Pharmacology | en_US |
dc.subject.mesh | Hypertension - Physiopathology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Muscle Contraction - Drug Effects - Physiology | en_US |
dc.subject.mesh | Muscle, Smooth, Vascular - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Nadph Dehydrogenase - Metabolism | en_US |
dc.subject.mesh | Ng-Nitroarginine Methyl Ester | en_US |
dc.subject.mesh | Nitric Oxide - Metabolism - Secretion | en_US |
dc.subject.mesh | Phenylephrine - Pharmacology | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Inbred Shr | en_US |
dc.subject.mesh | Rats, Inbred Wky | en_US |
dc.subject.mesh | Signal Transduction - Drug Effects - Physiology | en_US |
dc.title | Nonendothelial aortic source of nitric oxide in Wistar-Kyoto normotensive and spontaneous hypertensive rats. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Bourreau, JP:bourreau@hkucc.hku.hk | en_US |
dc.identifier.authority | Bourreau, JP=rp00389 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1159/000109337 | - |
dc.identifier.pmid | 1307932 | - |
dc.identifier.volume | 1 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 322 | en_US |
dc.identifier.epage | 330 | en_US |
dc.publisher.place | Switzerland | en_US |
dc.identifier.scopusauthorid | Bourreau, JP=7003927886 | en_US |
dc.identifier.scopusauthorid | Kitchener, P=35549211200 | en_US |
dc.identifier.scopusauthorid | Kwan, CY=7201421224 | en_US |
dc.identifier.scopusauthorid | Daniel, EE=35474017600 | en_US |
dc.identifier.issnl | 1016-0922 | - |