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- Publisher Website: 10.1016/S0008-6363(01)00498-9
- Scopus: eid_2-s2.0-0037083820
- PMID: 11861033
- WOS: WOS:000174980700011
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Article: Isoproterenol amplifies 17β-estradiol-mediated vasorelaxation: Role of endothelium/nitric oxide and cyclic AMP
Title | Isoproterenol amplifies 17β-estradiol-mediated vasorelaxation: Role of endothelium/nitric oxide and cyclic AMP |
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Authors | |
Keywords | Adrenergic (ant)agonists Arteries Endothelial function Hormones Vasoconstriction/dilation |
Issue Date | 2002 |
Publisher | Oxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.org |
Citation | Cardiovascular Research, 2002, v. 53 n. 3, p. 627-633 How to Cite? |
Abstract | Objectives: Estrogen exerts cardiac protection via multiple cellular mechanisms. Estrogen modifies vasodilatation induced by certain relaxants such as β-adrenoceptor agonists. However, little is known whether low concentrations of β-adrenoceptor agonists would reciprocally influence the acute relaxant response to estrogen. The present study was designed to investigate the synergistic interaction between isoproterenol and 17β-estradiol, and the role of endothelium and cyclic AMP-dependent pathway in this interaction. Methods: Changes in vessel tone of the isolated rat mesenteric artery rings were measured using a force-displacement Grass transducer. Results: In 9,11-dideoxy-11α, 9α-epoxy-methanoprostaglandin F 2α-preconstricted endothelium-intact rings, 17β-estradiol induced relaxations with pD 2 of 5.06±0.06. Pretreatment of endothelium-intact rings with isoproterenol (1-3×10 -9 M, 1 h incubation time) significantly enhanced 17β-estradiol-induced relaxation. This effect was inhibited by Rp-cGMPS triethylamine (3×10 -6 M), and abolished in the presence of 3×10 -5 M N G-nitro-L-arginine methyl ester or in endothelium-denuded rings. The effect of isoproterenol was antagonized by propranolol (3×10 -6 M), ICI 118,551 (3×10 -6 M), but not by atenolol (10 -5 M). Rp-cAMPS triethylamine (3×10 -6 M) abolished the effect of isoproterenol. Besides, exposure to 3×10 -9 M forskolin for 1 h also potentiated the relaxant response to 17β-estradiol. Conclusion: In endothelium-intact rat mesenteric arteries pretreatment with low concentrations of isoproterenol enhanced the acute relaxant response to 17β-estradiol. This enhancement was dependent on the presence of endothelium and abolished by L-NAME via a β 2-adrenoceptor-mediated cyclic AMP-dependent mechanism. © 2002 Elsevier Science B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/171709 |
ISSN | 2023 Impact Factor: 10.2 2023 SCImago Journal Rankings: 2.809 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chan, HY | en_US |
dc.contributor.author | Yao, X | en_US |
dc.contributor.author | Tsang, SY | en_US |
dc.contributor.author | Bourreau, JP | en_US |
dc.contributor.author | Chan, FL | en_US |
dc.contributor.author | Huang, Y | en_US |
dc.date.accessioned | 2012-10-30T06:16:32Z | - |
dc.date.available | 2012-10-30T06:16:32Z | - |
dc.date.issued | 2002 | en_US |
dc.identifier.citation | Cardiovascular Research, 2002, v. 53 n. 3, p. 627-633 | en_US |
dc.identifier.issn | 0008-6363 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171709 | - |
dc.description.abstract | Objectives: Estrogen exerts cardiac protection via multiple cellular mechanisms. Estrogen modifies vasodilatation induced by certain relaxants such as β-adrenoceptor agonists. However, little is known whether low concentrations of β-adrenoceptor agonists would reciprocally influence the acute relaxant response to estrogen. The present study was designed to investigate the synergistic interaction between isoproterenol and 17β-estradiol, and the role of endothelium and cyclic AMP-dependent pathway in this interaction. Methods: Changes in vessel tone of the isolated rat mesenteric artery rings were measured using a force-displacement Grass transducer. Results: In 9,11-dideoxy-11α, 9α-epoxy-methanoprostaglandin F 2α-preconstricted endothelium-intact rings, 17β-estradiol induced relaxations with pD 2 of 5.06±0.06. Pretreatment of endothelium-intact rings with isoproterenol (1-3×10 -9 M, 1 h incubation time) significantly enhanced 17β-estradiol-induced relaxation. This effect was inhibited by Rp-cGMPS triethylamine (3×10 -6 M), and abolished in the presence of 3×10 -5 M N G-nitro-L-arginine methyl ester or in endothelium-denuded rings. The effect of isoproterenol was antagonized by propranolol (3×10 -6 M), ICI 118,551 (3×10 -6 M), but not by atenolol (10 -5 M). Rp-cAMPS triethylamine (3×10 -6 M) abolished the effect of isoproterenol. Besides, exposure to 3×10 -9 M forskolin for 1 h also potentiated the relaxant response to 17β-estradiol. Conclusion: In endothelium-intact rat mesenteric arteries pretreatment with low concentrations of isoproterenol enhanced the acute relaxant response to 17β-estradiol. This enhancement was dependent on the presence of endothelium and abolished by L-NAME via a β 2-adrenoceptor-mediated cyclic AMP-dependent mechanism. © 2002 Elsevier Science B.V. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Oxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.org | en_US |
dc.relation.ispartof | Cardiovascular Research | en_US |
dc.rights | Cardiovascular Research. Copyright © Elsevier BV. | - |
dc.subject | Adrenergic (ant)agonists | - |
dc.subject | Arteries | - |
dc.subject | Endothelial function | - |
dc.subject | Hormones | - |
dc.subject | Vasoconstriction/dilation | - |
dc.subject.mesh | Adrenergic Beta-Agonists - Pharmacology | en_US |
dc.subject.mesh | Adrenergic Beta-Antagonists - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Atenolol - Pharmacology | en_US |
dc.subject.mesh | Cyclic Amp - Metabolism | en_US |
dc.subject.mesh | Drug Synergism | en_US |
dc.subject.mesh | Endothelium, Vascular - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Enzyme Inhibitors - Pharmacology | en_US |
dc.subject.mesh | Estradiol - Pharmacology | en_US |
dc.subject.mesh | Isoproterenol - Pharmacology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Mesenteric Arteries | en_US |
dc.subject.mesh | Ng-Nitroarginine Methyl Ester - Pharmacology | en_US |
dc.subject.mesh | Nitric Oxide - Metabolism | en_US |
dc.subject.mesh | Nitric Oxide Synthase - Antagonists & Inhibitors | en_US |
dc.subject.mesh | Propanolamines - Pharmacology | en_US |
dc.subject.mesh | Propranolol - Pharmacology | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Vasodilation - Drug Effects | en_US |
dc.title | Isoproterenol amplifies 17β-estradiol-mediated vasorelaxation: Role of endothelium/nitric oxide and cyclic AMP | en_US |
dc.type | Article | en_US |
dc.identifier.email | Bourreau, JP:bourreau@hkucc.hku.hk | en_US |
dc.identifier.authority | Bourreau, JP=rp00389 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0008-6363(01)00498-9 | en_US |
dc.identifier.pmid | 11861033 | - |
dc.identifier.scopus | eid_2-s2.0-0037083820 | en_US |
dc.identifier.hkuros | 82112 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037083820&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 53 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 627 | en_US |
dc.identifier.epage | 633 | en_US |
dc.identifier.isi | WOS:000174980700011 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Chan, HY=7403402342 | en_US |
dc.identifier.scopusauthorid | Yao, X=7402529434 | en_US |
dc.identifier.scopusauthorid | Tsang, SY=7102255908 | en_US |
dc.identifier.scopusauthorid | Bourreau, JP=7003927886 | en_US |
dc.identifier.scopusauthorid | Chan, FL=15050111400 | en_US |
dc.identifier.scopusauthorid | Huang, Y=7501573013 | en_US |
dc.identifier.issnl | 0008-6363 | - |