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Article: Analysis of submicromolar concentrations of adenosine in plasma using reversed phase high-performance liquid chromatography
Title | Analysis of submicromolar concentrations of adenosine in plasma using reversed phase high-performance liquid chromatography |
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Authors | |
Keywords | Adenosine Reversed-Phase High-Performance Liquid Chromatography. Vasodilators |
Issue Date | 1986 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba |
Citation | Journal Of Pharmaceutical And Biomedical Analysis, 1986, v. 4 n. 2, p. 207-219 How to Cite? |
Abstract | A method is described for the determination of adenosine in small samples of plasma (< 1 ml) using reversed-phase high-performance liquid chromatography (HPLC) in either a simple isocratic or a gradient elution system which gives a clear separation of adenosine from other plasma constituents. Acetone is used to deproteinize plasma and chloroform to remove unwanted lipid soluble material prior to HPLC. 6-Methyladenosine is used as an internal standard for making corrections for changes in concentration during sample processing. Adenosine in plasma could be reliably detected at concentrations lower than its minimum effector concentration as a vasodilator (4 × 10-8 Mol l-1 using the isocratic system and 1.9 × 10-8 Mol l-1 with gradient elution). The recoveries of adenosine added to blood at concentrations ranging from 2 × 10-8 Mol l-1 to 1.4 × 10-6 Mol l-1 were from 101.4 ± 16.9% (n = 4) to 100.0 ± 3.6% (n = 5). The present method provides a simple, sensitive and selective assay for submicromolar concentrations of adenosine in plasma with good recovery. © 1986. |
Persistent Identifier | http://hdl.handle.net/10722/171761 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.584 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ballard, HJ | en_US |
dc.contributor.author | Cotterrell, D | en_US |
dc.contributor.author | Karim, F | en_US |
dc.date.accessioned | 2012-10-30T06:16:50Z | - |
dc.date.available | 2012-10-30T06:16:50Z | - |
dc.date.issued | 1986 | en_US |
dc.identifier.citation | Journal Of Pharmaceutical And Biomedical Analysis, 1986, v. 4 n. 2, p. 207-219 | en_US |
dc.identifier.issn | 0731-7085 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171761 | - |
dc.description.abstract | A method is described for the determination of adenosine in small samples of plasma (< 1 ml) using reversed-phase high-performance liquid chromatography (HPLC) in either a simple isocratic or a gradient elution system which gives a clear separation of adenosine from other plasma constituents. Acetone is used to deproteinize plasma and chloroform to remove unwanted lipid soluble material prior to HPLC. 6-Methyladenosine is used as an internal standard for making corrections for changes in concentration during sample processing. Adenosine in plasma could be reliably detected at concentrations lower than its minimum effector concentration as a vasodilator (4 × 10-8 Mol l-1 using the isocratic system and 1.9 × 10-8 Mol l-1 with gradient elution). The recoveries of adenosine added to blood at concentrations ranging from 2 × 10-8 Mol l-1 to 1.4 × 10-6 Mol l-1 were from 101.4 ± 16.9% (n = 4) to 100.0 ± 3.6% (n = 5). The present method provides a simple, sensitive and selective assay for submicromolar concentrations of adenosine in plasma with good recovery. © 1986. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba | en_US |
dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis | en_US |
dc.subject | Adenosine | en_US |
dc.subject | Reversed-Phase High-Performance Liquid Chromatography. | en_US |
dc.subject | Vasodilators | en_US |
dc.title | Analysis of submicromolar concentrations of adenosine in plasma using reversed phase high-performance liquid chromatography | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ballard, HJ:ballard@hkucc.hku.hk | en_US |
dc.identifier.authority | Ballard, HJ=rp00367 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.scopus | eid_2-s2.0-38249043986 | en_US |
dc.identifier.volume | 4 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 207 | en_US |
dc.identifier.epage | 219 | en_US |
dc.identifier.isi | WOS:A1986C951700009 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Ballard, HJ=7005286310 | en_US |
dc.identifier.scopusauthorid | Cotterrell, D=6602879005 | en_US |
dc.identifier.scopusauthorid | Karim, F=7005896790 | en_US |
dc.identifier.issnl | 0731-7085 | - |