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- Publisher Website: 10.1167/iovs.11-7639
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- PMID: 21743019
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Article: Evaluation of proteoglycan gene polymorphisms as risk factors in the genetic susceptibility to high myopia
Title | Evaluation of proteoglycan gene polymorphisms as risk factors in the genetic susceptibility to high myopia |
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Authors | |
Issue Date | 2011 |
Publisher | Association for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org |
Citation | Investigative Ophthalmology And Visual Science, 2011, v. 52 n. 9, p. 6396-6403 How to Cite? |
Abstract | Purpose. To investigate the relationship between high myopia and single nucleotide polymorphisms (SNPs) in six proteoglycan genes: aggrecan (ACAN), fibromodulin (FMOD), decorin (DCN), lumican (LUM), keratocan (KERA), and epiphycan (EPYC). These genes were selected for study because they are involved in induced myopia in animals and/or are within the human MYP3 locus identified by linkage analysis of families with high myopia. Methods. Two groups of Chinese subjects were studied: group 1 (300 cases and 300 controls) and group 2 (356 cases and 354 controls). Cases were high myopes with spherical equivalent (SE) ≤ -8.00 D, and controls had SE between +1.0 and -1.0 D. From these candidate genes, 60 tagging SNPs were selected. First, 12 DNA pools were each constructed from 50 samples of the same phenotype from group 1 subjects and were tested for association with the SNPs. Second, putatively positive SNPs were confirmed by individual genotyping of group 1 subjects. Finally, positive results were replicated in group 2 subjects. Results. Of the 58 SNPs successfully screened by DNA pooling, 8 ACAN SNPs passed the threshold of P ≤ 0.10 (nested ANOVA) and were then genotyped in the individual samples. Haplotypes rs3784757 and rs1516794 showed significant association with high myopia. However, the positive result could not be replicated in the second subject group. Conclusions. These six proteoglycan genes were not associated with high myopia in these Chinese subjects and hence are unlikely to be important in the genetic predisposition to high myopia. © 2011 The Association for Research in Vision and Ophthalmology, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/175987 |
ISSN | 2023 Impact Factor: 5.0 2023 SCImago Journal Rankings: 1.422 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yip, SP | en_US |
dc.contributor.author | Leung, KH | en_US |
dc.contributor.author | Ng, PW | en_US |
dc.contributor.author | Fung, WY | en_US |
dc.contributor.author | Sham, PC | en_US |
dc.contributor.author | Yap, MKH | en_US |
dc.date.accessioned | 2012-11-26T09:03:19Z | - |
dc.date.available | 2012-11-26T09:03:19Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | Investigative Ophthalmology And Visual Science, 2011, v. 52 n. 9, p. 6396-6403 | en_US |
dc.identifier.issn | 0146-0404 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/175987 | - |
dc.description.abstract | Purpose. To investigate the relationship between high myopia and single nucleotide polymorphisms (SNPs) in six proteoglycan genes: aggrecan (ACAN), fibromodulin (FMOD), decorin (DCN), lumican (LUM), keratocan (KERA), and epiphycan (EPYC). These genes were selected for study because they are involved in induced myopia in animals and/or are within the human MYP3 locus identified by linkage analysis of families with high myopia. Methods. Two groups of Chinese subjects were studied: group 1 (300 cases and 300 controls) and group 2 (356 cases and 354 controls). Cases were high myopes with spherical equivalent (SE) ≤ -8.00 D, and controls had SE between +1.0 and -1.0 D. From these candidate genes, 60 tagging SNPs were selected. First, 12 DNA pools were each constructed from 50 samples of the same phenotype from group 1 subjects and were tested for association with the SNPs. Second, putatively positive SNPs were confirmed by individual genotyping of group 1 subjects. Finally, positive results were replicated in group 2 subjects. Results. Of the 58 SNPs successfully screened by DNA pooling, 8 ACAN SNPs passed the threshold of P ≤ 0.10 (nested ANOVA) and were then genotyped in the individual samples. Haplotypes rs3784757 and rs1516794 showed significant association with high myopia. However, the positive result could not be replicated in the second subject group. Conclusions. These six proteoglycan genes were not associated with high myopia in these Chinese subjects and hence are unlikely to be important in the genetic predisposition to high myopia. © 2011 The Association for Research in Vision and Ophthalmology, Inc. | en_US |
dc.language | eng | en_US |
dc.publisher | Association for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org | en_US |
dc.relation.ispartof | Investigative Ophthalmology and Visual Science | en_US |
dc.title | Evaluation of proteoglycan gene polymorphisms as risk factors in the genetic susceptibility to high myopia | en_US |
dc.type | Article | en_US |
dc.identifier.email | Sham, PC: pcsham@hku.hk | en_US |
dc.identifier.authority | Sham, PC=rp00459 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1167/iovs.11-7639 | en_US |
dc.identifier.pmid | 21743019 | - |
dc.identifier.scopus | eid_2-s2.0-80054025649 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-80054025649&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 52 | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.spage | 6396 | en_US |
dc.identifier.epage | 6403 | en_US |
dc.identifier.isi | WOS:000294548300006 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Yip, SP=7102133673 | en_US |
dc.identifier.scopusauthorid | Leung, KH=36946027000 | en_US |
dc.identifier.scopusauthorid | Ng, PW=16199988300 | en_US |
dc.identifier.scopusauthorid | Fung, WY=25958608900 | en_US |
dc.identifier.scopusauthorid | Sham, PC=34573429300 | en_US |
dc.identifier.scopusauthorid | Yap, MKH=7006673734 | en_US |
dc.identifier.issnl | 0146-0404 | - |