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- Publisher Website: 10.1159/000125408
- Scopus: eid_2-s2.0-0025348520
- PMID: 2141920
- WOS: WOS:A1990DJ27600009
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Article: Differential actions of dopamine receptor subtypes on gonadotropin and growth hormone release in vitro in goldfish
Title | Differential actions of dopamine receptor subtypes on gonadotropin and growth hormone release in vitro in goldfish |
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Authors | |
Keywords | Dispersed pituitary cells Dopamine D1 and D2 receptors Goldfish Gonadotropin Gonadotropin-releasing hormone action Growth hormone Perifusion Static incubation |
Issue Date | 1990 |
Publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/NEN |
Citation | Neuroendocrinology, 1990, v. 51 n. 6, p. 664-674 How to Cite? |
Abstract | Incubation of cultured goldfish pituitary cells with 10 nM to 1 μM apomorphine (APO), a non-selective dopamine agonist, increased growth hormone (GH) release in a dose-dependent manner. GH release was also stimulated in a dose-dependent manner by 0.1 nM to 1 μM salmon gonadotropin (GTH)-releasing hormone (sGnRH), sGnRH analog, and chicken GnRH-II (cGnRH-II). The magnitude of GH responses to 1 μM GnRHs were less than that to 1 μM APO. GH responses to 10 nM to 1 μM APO were not significantly increased by the addition of GnRHs. Static incubations with 0.1 nM to 1 μM of the dopamine D1 agonist SKF38393 did not alter basal GTH release, or the GTH responses to 10 nM sGnRH and cGnRH-II. In contrast, the D1 agonist SKF38393 significantly increased basal GH secretion with maximal stimulation achieved at 100 nM concentration, and GH responses to 10 nM sGnRH and 10 nM cGnRH-II were enhanced by simultaneous applications of SKF38393. Incubation with 1 μM of the D2 agonist LY171555 decreased basal GTH release. Additions of 0.1 nM to 1 μM LY171555 caused dose-dependent decreases in the GTH secretion induced by 10 nM sGnRH and cGnR-II. In contrast, basal and GnRH-stimulated GH release were not affected by coincubations with LY171555. The D1 antagonist SKF83566 and the D2 antagonist domperidone, at 1 μM concentrations, specifically blocked the D1 agonist SKF38393-stimulated increase in GH release and the D2 agonist LY171555-induced depression of GTH secretion, respectively. In cell column perifusion studies, the D1 agonist SKF38393 at 0.1 nM to 1 μM had no effects on GTH release, but significantly elevated GH secretion rates when applied at 0.1-1 μM concentrations. The GH release induced by 1 μM SKF38393 was significantly reduced by simultaneous perifusion with 1 μM of the D1 antagonist SKF83566. Treatments with SKF38393 and/or SKF83566 did not affect net GTH and GH responses to sGnRH challenges. In contrast, perifusion with 0.1 and 1 μM of the D2 agonist LY171555 depressed basal as well as sGnRH-induced GTH responses. These effects of 1 μM LY171555 were completely blocked by simultaneous applications of 1 μM domperidone, a D2 antagonist. Treatments with these D2 selective drugs did not affect basal and sGnRH-stimulated GH release. These results indicate that in cultured goldfish pituitary cells, activation of dopamine D1- and D2-like receptors specifically stimulates GH release and inhibits both basal and stimulated GTH secretion, respectively. The presence of direct actions of sGnRH and cGnRH-II on goldfish GTH and GH release are also demonstrated. This is the first study do demonstrate stimulatory dopamine D1 actions in the vertebrate pituitary. |
Persistent Identifier | http://hdl.handle.net/10722/178494 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 1.009 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chang, JP | en_US |
dc.contributor.author | Yu, KL | en_US |
dc.contributor.author | Wong, AOL | en_US |
dc.contributor.author | Peter, RE | en_US |
dc.date.accessioned | 2012-12-19T09:48:01Z | - |
dc.date.available | 2012-12-19T09:48:01Z | - |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Neuroendocrinology, 1990, v. 51 n. 6, p. 664-674 | en_US |
dc.identifier.issn | 0028-3835 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178494 | - |
dc.description.abstract | Incubation of cultured goldfish pituitary cells with 10 nM to 1 μM apomorphine (APO), a non-selective dopamine agonist, increased growth hormone (GH) release in a dose-dependent manner. GH release was also stimulated in a dose-dependent manner by 0.1 nM to 1 μM salmon gonadotropin (GTH)-releasing hormone (sGnRH), sGnRH analog, and chicken GnRH-II (cGnRH-II). The magnitude of GH responses to 1 μM GnRHs were less than that to 1 μM APO. GH responses to 10 nM to 1 μM APO were not significantly increased by the addition of GnRHs. Static incubations with 0.1 nM to 1 μM of the dopamine D1 agonist SKF38393 did not alter basal GTH release, or the GTH responses to 10 nM sGnRH and cGnRH-II. In contrast, the D1 agonist SKF38393 significantly increased basal GH secretion with maximal stimulation achieved at 100 nM concentration, and GH responses to 10 nM sGnRH and 10 nM cGnRH-II were enhanced by simultaneous applications of SKF38393. Incubation with 1 μM of the D2 agonist LY171555 decreased basal GTH release. Additions of 0.1 nM to 1 μM LY171555 caused dose-dependent decreases in the GTH secretion induced by 10 nM sGnRH and cGnR-II. In contrast, basal and GnRH-stimulated GH release were not affected by coincubations with LY171555. The D1 antagonist SKF83566 and the D2 antagonist domperidone, at 1 μM concentrations, specifically blocked the D1 agonist SKF38393-stimulated increase in GH release and the D2 agonist LY171555-induced depression of GTH secretion, respectively. In cell column perifusion studies, the D1 agonist SKF38393 at 0.1 nM to 1 μM had no effects on GTH release, but significantly elevated GH secretion rates when applied at 0.1-1 μM concentrations. The GH release induced by 1 μM SKF38393 was significantly reduced by simultaneous perifusion with 1 μM of the D1 antagonist SKF83566. Treatments with SKF38393 and/or SKF83566 did not affect net GTH and GH responses to sGnRH challenges. In contrast, perifusion with 0.1 and 1 μM of the D2 agonist LY171555 depressed basal as well as sGnRH-induced GTH responses. These effects of 1 μM LY171555 were completely blocked by simultaneous applications of 1 μM domperidone, a D2 antagonist. Treatments with these D2 selective drugs did not affect basal and sGnRH-stimulated GH release. These results indicate that in cultured goldfish pituitary cells, activation of dopamine D1- and D2-like receptors specifically stimulates GH release and inhibits both basal and stimulated GTH secretion, respectively. The presence of direct actions of sGnRH and cGnRH-II on goldfish GTH and GH release are also demonstrated. This is the first study do demonstrate stimulatory dopamine D1 actions in the vertebrate pituitary. | en_US |
dc.language | eng | en_US |
dc.publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/NEN | en_US |
dc.relation.ispartof | Neuroendocrinology | en_US |
dc.subject | Dispersed pituitary cells | - |
dc.subject | Dopamine D1 and D2 receptors | - |
dc.subject | Goldfish | - |
dc.subject | Gonadotropin | - |
dc.subject | Gonadotropin-releasing hormone action | - |
dc.subject | Growth hormone | - |
dc.subject | Perifusion | - |
dc.subject | Static incubation | - |
dc.subject.mesh | 2,3,4,5-Tetrahydro-7,8-Dihydroxy-1-Phenyl-1H-3-Benzazepine - Analogs & Derivatives - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Apomorphine - Pharmacology | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Cyprinidae - Physiology | en_US |
dc.subject.mesh | Domperidone - Pharmacology | en_US |
dc.subject.mesh | Dopamine Antagonists | en_US |
dc.subject.mesh | Ergolines - Pharmacology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Goldfish - Physiology | en_US |
dc.subject.mesh | Gonadotropins, Pituitary - Secretion | en_US |
dc.subject.mesh | Growth Hormone - Secretion | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Perfusion | en_US |
dc.subject.mesh | Pituitary Gland - Drug Effects - Secretion | en_US |
dc.subject.mesh | Pituitary Hormone-Releasing Hormones - Pharmacology | en_US |
dc.subject.mesh | Quinpirole | en_US |
dc.subject.mesh | Receptors, Dopamine - Physiology | en_US |
dc.subject.mesh | Receptors, Dopamine D1 | en_US |
dc.subject.mesh | Receptors, Dopamine D2 | en_US |
dc.title | Differential actions of dopamine receptor subtypes on gonadotropin and growth hormone release in vitro in goldfish | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wong, AOL: olwong@hkucc.hku.hk | en_US |
dc.identifier.authority | Wong, AOL=rp00806 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1159/000125408 | - |
dc.identifier.pmid | 2141920 | - |
dc.identifier.scopus | eid_2-s2.0-0025348520 | en_US |
dc.identifier.volume | 51 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 664 | en_US |
dc.identifier.epage | 674 | en_US |
dc.identifier.isi | WOS:A1990DJ27600009 | - |
dc.publisher.place | Switzerland | en_US |
dc.identifier.scopusauthorid | Chang, JP=7601547649 | en_US |
dc.identifier.scopusauthorid | Yu, KL=7403385265 | en_US |
dc.identifier.scopusauthorid | Wong, AOL=7403147570 | en_US |
dc.identifier.scopusauthorid | Peter, RE=7202909690 | en_US |
dc.identifier.issnl | 0028-3835 | - |