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- Publisher Website: 10.1016/0196-9781(90)90132-O
- Scopus: eid_2-s2.0-0025634263
- PMID: 2087431
- WOS: WOS:A1990EQ00800003
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Article: Expression of recombinant human glucose-dependent insulinotropic polypeptide in Escherichia coli by sequence-specific proteolysis of a protein A fusion protein
Title | Expression of recombinant human glucose-dependent insulinotropic polypeptide in Escherichia coli by sequence-specific proteolysis of a protein A fusion protein |
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Authors | |
Keywords | Expression in E. coli Human GIP Protein A fusion protein Thrombin cleavage |
Issue Date | 1990 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/peptides |
Citation | Peptides, 1990, v. 11 n. 6, p. 1069-1074 How to Cite? |
Abstract | Glucose-dependent insulinotropic polypeptide (GIP) is a forty-two amino acid hormone that stimulates the secretion of insulin from the pancreatic B-cells in the presence of elevated glucose concentrations. The human GIP gene with the human Aα-fibrinopeptide sequence was synthesized and linked to the Staphylococcus aureus protein A gene in the vector pRIT2T. This plasmid was expressed in Escherichia coli, and the resulting fusion protein consisted of three domains: protein A for ease of purification, fibrinopeptide sequence for thrombin cleavage and human GIP. The GIP was subsequently cleaved from the fusion protein with α-thrombin. The identity of the recombinant human GIP was confirmed by SDS-PAGE, ELISA, HPLC and amino-terminal amino acid sequence analysis. This recombinant product was shown to have comparable insulinotropic activity to porcine GIP in the isolated perfused pancreas. |
Persistent Identifier | http://hdl.handle.net/10722/178501 |
ISSN | 2023 Impact Factor: 2.8 2023 SCImago Journal Rankings: 0.826 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chow, BKC | en_US |
dc.contributor.author | Morrow, GW | en_US |
dc.contributor.author | Ho, M | en_US |
dc.contributor.author | Pederson, RA | en_US |
dc.contributor.author | Mcintosh, CHS | en_US |
dc.contributor.author | Brown, JC | en_US |
dc.contributor.author | Macgillivray, RTA | en_US |
dc.date.accessioned | 2012-12-19T09:48:03Z | - |
dc.date.available | 2012-12-19T09:48:03Z | - |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Peptides, 1990, v. 11 n. 6, p. 1069-1074 | en_US |
dc.identifier.issn | 0196-9781 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178501 | - |
dc.description.abstract | Glucose-dependent insulinotropic polypeptide (GIP) is a forty-two amino acid hormone that stimulates the secretion of insulin from the pancreatic B-cells in the presence of elevated glucose concentrations. The human GIP gene with the human Aα-fibrinopeptide sequence was synthesized and linked to the Staphylococcus aureus protein A gene in the vector pRIT2T. This plasmid was expressed in Escherichia coli, and the resulting fusion protein consisted of three domains: protein A for ease of purification, fibrinopeptide sequence for thrombin cleavage and human GIP. The GIP was subsequently cleaved from the fusion protein with α-thrombin. The identity of the recombinant human GIP was confirmed by SDS-PAGE, ELISA, HPLC and amino-terminal amino acid sequence analysis. This recombinant product was shown to have comparable insulinotropic activity to porcine GIP in the isolated perfused pancreas. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/peptides | en_US |
dc.relation.ispartof | Peptides | en_US |
dc.subject | Expression in E. coli | - |
dc.subject | Human GIP | - |
dc.subject | Protein A fusion protein | - |
dc.subject | Thrombin cleavage | - |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Biological Assay | en_US |
dc.subject.mesh | Escherichia Coli - Metabolism | en_US |
dc.subject.mesh | Gastric Inhibitory Polypeptide - Biosynthesis | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Pancreas - Chemistry | en_US |
dc.subject.mesh | Perfusion | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Inbred Strains | en_US |
dc.subject.mesh | Recombinant Fusion Proteins - Metabolism | en_US |
dc.subject.mesh | Recombinant Proteins - Biosynthesis | en_US |
dc.subject.mesh | Staphylococcal Protein A - Metabolism | en_US |
dc.subject.mesh | Substrate Specificity | en_US |
dc.subject.mesh | Thrombin | en_US |
dc.title | Expression of recombinant human glucose-dependent insulinotropic polypeptide in Escherichia coli by sequence-specific proteolysis of a protein A fusion protein | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chow, BKC: bkcc@hku.hk | en_US |
dc.identifier.authority | Chow, BKC=rp00681 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0196-9781(90)90132-O | en_US |
dc.identifier.pmid | 2087431 | - |
dc.identifier.scopus | eid_2-s2.0-0025634263 | en_US |
dc.identifier.volume | 11 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 1069 | en_US |
dc.identifier.epage | 1074 | en_US |
dc.identifier.isi | WOS:A1990EQ00800003 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Chow, BKC=7102826193 | en_US |
dc.identifier.scopusauthorid | Morrow, GW=7102277890 | en_US |
dc.identifier.scopusauthorid | Ho, M=7403080604 | en_US |
dc.identifier.scopusauthorid | Pederson, RA=7005815440 | en_US |
dc.identifier.scopusauthorid | McIntosh, CHS=7103076827 | en_US |
dc.identifier.scopusauthorid | Brown, JC=7409452569 | en_US |
dc.identifier.scopusauthorid | MacGillivray, RTA=7004286039 | en_US |
dc.identifier.issnl | 0196-9781 | - |