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- Publisher Website: 10.1007/BF00556293
- Scopus: eid_2-s2.0-0026129638
- PMID: 1367376
- WOS: WOS:A1991FN53400006
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Article: High density cultivation of BSK cells on sintered alumina ceramic foam support
Title | High density cultivation of BSK cells on sintered alumina ceramic foam support |
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Authors | |
Keywords | BHK ceramic high cell density immobilization perfusion system |
Issue Date | 1991 |
Publisher | Springer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-9069 |
Citation | Cytotechnology, 1991, v. 5 n. 3, p. 233-241 How to Cite? |
Abstract | A perfusion system which utilizes a porous ceramic core has been tested for the cultivation of transformed BHK cells which produce human transferrin. A design is presented in which cells are immobilized within the porous ceramic particle and are fed by continuous perfusion of batch liquid medium. It was found that more than 5×109 BHK cells could be supported within the 40 mL ceramic matrix, a ten-fold increase in cell density per unit surface area over the standard roller bottle cultures or a five-fold increase in volumetric cell density over suspension cultures. The cell specific productivity of human transferrin was similar to that observed in suspension culture. The system offers the advantages of significant reduction in serum requirements and the potential for scale-up. © 1991 Kluwer Academic Publishers. |
Persistent Identifier | http://hdl.handle.net/10722/178515 |
ISSN | 2023 Impact Factor: 2.0 2023 SCImago Journal Rankings: 0.485 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Lee, DW | en_US |
dc.contributor.author | Grace, JR | en_US |
dc.contributor.author | Chow, BKC | en_US |
dc.contributor.author | Macgillivray, RTA | en_US |
dc.contributor.author | Kilburn, DG | en_US |
dc.date.accessioned | 2012-12-19T09:48:09Z | - |
dc.date.available | 2012-12-19T09:48:09Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Cytotechnology, 1991, v. 5 n. 3, p. 233-241 | en_US |
dc.identifier.issn | 0920-9069 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178515 | - |
dc.description.abstract | A perfusion system which utilizes a porous ceramic core has been tested for the cultivation of transformed BHK cells which produce human transferrin. A design is presented in which cells are immobilized within the porous ceramic particle and are fed by continuous perfusion of batch liquid medium. It was found that more than 5×109 BHK cells could be supported within the 40 mL ceramic matrix, a ten-fold increase in cell density per unit surface area over the standard roller bottle cultures or a five-fold increase in volumetric cell density over suspension cultures. The cell specific productivity of human transferrin was similar to that observed in suspension culture. The system offers the advantages of significant reduction in serum requirements and the potential for scale-up. © 1991 Kluwer Academic Publishers. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-9069 | en_US |
dc.relation.ispartof | Cytotechnology | en_US |
dc.subject | BHK | - |
dc.subject | ceramic | - |
dc.subject | high cell density | - |
dc.subject | immobilization | - |
dc.subject | perfusion system | - |
dc.subject.mesh | Aluminum Oxide | en_US |
dc.subject.mesh | Cell Count | en_US |
dc.subject.mesh | Cell Division | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Ceramics | en_US |
dc.subject.mesh | Cloning, Molecular - Methods | en_US |
dc.subject.mesh | Glucose - Metabolism | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Lactates - Biosynthesis | en_US |
dc.subject.mesh | Oxygen Consumption | en_US |
dc.subject.mesh | Perfusion | en_US |
dc.subject.mesh | Porosity | en_US |
dc.subject.mesh | Transferrin - Biosynthesis | en_US |
dc.title | High density cultivation of BSK cells on sintered alumina ceramic foam support | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chow, BKC: bkcc@hku.hk | en_US |
dc.identifier.authority | Chow, BKC=rp00681 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/BF00556293 | en_US |
dc.identifier.pmid | 1367376 | en_US |
dc.identifier.scopus | eid_2-s2.0-0026129638 | en_US |
dc.identifier.volume | 5 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 233 | en_US |
dc.identifier.epage | 241 | en_US |
dc.identifier.isi | WOS:A1991FN53400006 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Lee, DW=16151945000 | en_US |
dc.identifier.scopusauthorid | Grace, JR=7202870990 | en_US |
dc.identifier.scopusauthorid | Chow, BKC=7102826193 | en_US |
dc.identifier.scopusauthorid | MacGillivray, RTA=7004286039 | en_US |
dc.identifier.scopusauthorid | Kilburn, DG=35500772400 | en_US |
dc.identifier.issnl | 0920-9069 | - |