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- Publisher Website: 10.1094/MPMI.1999.12.6.479
- Scopus: eid_2-s2.0-0033151721
- PMID: 10356799
- WOS: WOS:000080383500001
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Article: cDNA cloning of a sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum
Title | cDNA cloning of a sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum |
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Authors | |
Keywords | 3-deoxyanthocyanidin Anthracnose Bipolaris maydis Incompatible interactions |
Issue Date | 1999 |
Publisher | American Phytopathological Society. The Journal's web site is located at http://www.apsnet.org/mpmi |
Citation | Molecular Plant-Microbe Interactions, 1999, v. 12 n. 6, p. 479-489 How to Cite? |
Abstract | A sorghum cDNA clone was isolated by differential screening of a cDNA library prepared from mesocotyls (cultivar DK18) inoculated with fungal pathogens. The deduced translation product shows sequence similarity to a family of intracellular pathogenesis-related proteins (PR-10) with a potential ribonuclease function. We studied the accumulation of PR-10 and chalcone synthase (CHS) transcripts in mesocotyls following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum. CHS is involved in phytoalexin synthesis in sorghum. Coordinate expression of PR-10 and CHS genes was localized in the area of inoculation along with the accumulation of phytoalexins. C. heterostrophus is a nonpathogen of sorghum and cytological studies indicated that cultivar DK18 is resistant to C. sublineolum, a sorghum pathogen. We demonstrated that the two fungi triggered different time courses of plant defense reactions. Inoculation with C. heterostrophus resulted in rapid accumulation of PR-10 and CHS transcripts after appressoria had become mature. Accumulation of these transcripts was delayed in plants inoculated with C. sublineolum until penetration of host tissue had been completed and infection vesicles had formed. Results suggest that different recognition events are involved in the expression of resistance to the two fungi used or that C. sublineolum suppresses the nonspecific induction of defense responses. |
Persistent Identifier | http://hdl.handle.net/10722/178676 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 0.932 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Lo, SCC | en_US |
dc.contributor.author | Hipskind, JD | en_US |
dc.contributor.author | Nicholson, RL | en_US |
dc.date.accessioned | 2012-12-19T09:49:04Z | - |
dc.date.available | 2012-12-19T09:49:04Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Molecular Plant-Microbe Interactions, 1999, v. 12 n. 6, p. 479-489 | en_US |
dc.identifier.issn | 0894-0282 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178676 | - |
dc.description.abstract | A sorghum cDNA clone was isolated by differential screening of a cDNA library prepared from mesocotyls (cultivar DK18) inoculated with fungal pathogens. The deduced translation product shows sequence similarity to a family of intracellular pathogenesis-related proteins (PR-10) with a potential ribonuclease function. We studied the accumulation of PR-10 and chalcone synthase (CHS) transcripts in mesocotyls following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum. CHS is involved in phytoalexin synthesis in sorghum. Coordinate expression of PR-10 and CHS genes was localized in the area of inoculation along with the accumulation of phytoalexins. C. heterostrophus is a nonpathogen of sorghum and cytological studies indicated that cultivar DK18 is resistant to C. sublineolum, a sorghum pathogen. We demonstrated that the two fungi triggered different time courses of plant defense reactions. Inoculation with C. heterostrophus resulted in rapid accumulation of PR-10 and CHS transcripts after appressoria had become mature. Accumulation of these transcripts was delayed in plants inoculated with C. sublineolum until penetration of host tissue had been completed and infection vesicles had formed. Results suggest that different recognition events are involved in the expression of resistance to the two fungi used or that C. sublineolum suppresses the nonspecific induction of defense responses. | en_US |
dc.language | eng | en_US |
dc.publisher | American Phytopathological Society. The Journal's web site is located at http://www.apsnet.org/mpmi | en_US |
dc.relation.ispartof | Molecular Plant-Microbe Interactions | en_US |
dc.subject | 3-deoxyanthocyanidin | - |
dc.subject | Anthracnose | - |
dc.subject | Bipolaris maydis | - |
dc.subject | Incompatible interactions | - |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Ascomycota - Pathogenicity | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Cereals - Genetics - Microbiology | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Dna, Complementary | en_US |
dc.subject.mesh | Gene Expression Regulation, Plant | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Plant Proteins - Biosynthesis - Chemistry - Genetics | en_US |
dc.subject.mesh | Plants - Genetics | en_US |
dc.subject.mesh | Recombinant Proteins - Biosynthesis - Chemistry | en_US |
dc.subject.mesh | Sequence Alignment | en_US |
dc.subject.mesh | Sequence Homology, Amino Acid | en_US |
dc.title | cDNA cloning of a sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lo, SCC: clivelo@hkucc.hku.hk | en_US |
dc.identifier.authority | Lo, SCC=rp00751 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1094/MPMI.1999.12.6.479 | - |
dc.identifier.pmid | 10356799 | - |
dc.identifier.scopus | eid_2-s2.0-0033151721 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0033151721&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 12 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 479 | en_US |
dc.identifier.epage | 489 | en_US |
dc.identifier.isi | WOS:000080383500001 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Lo, SCC=15737175700 | en_US |
dc.identifier.scopusauthorid | Hipskind, JD=6603397831 | en_US |
dc.identifier.scopusauthorid | Nicholson, RL=7201624684 | en_US |
dc.identifier.issnl | 0894-0282 | - |